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Response surface methodology for optimizing the fermentation medium of alpha-galactosidase in solid-state fermentation

Response surface methodology for optimizing the fermentation...

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中国食品科学技术学会第五届年会暨第四届东西方食品业高层论坛

作者： C.Q.Liu Q.H.Chen B.Tang H.Ruan G.Q.He (Department of Food Science and Nutrition,Zhejiang University,Hangzhou 310029,China)

Aims:alpha-galactosidase is applied in food and feed industries for hydrolysing raffinose series oligosaccharides(RO)that are the factors primarily responsible for flatulence upon ingestion of soybean-derived *** objective of the current work was to develop an optimal culture medium for the production of alpha-galactosidase in solid-state fermentation(SSF) by a mutant strain Aspergillus foetidus.,Methods and Results:Response surface methodology (RSM)was applied to evaluate the effects of variables,namely the concentrations of wheat bran, soybean meal,KHPO,MnSO·HO and CuSO·5HO on alpha-galactosidase production in the solid substrate.A fractional factorial design(FFD)was firstly used to isolate the main factors that affected the production of alpha-galactosidase and the central composite experimental design (CCD)was then adopted to derive a statistical model for optimizing the composition of the fermentation *** experimental results showed that the optimum fermentation medium for alpha-galactosidase production by Aspergillus foetidus ZU-Gl was composed of 8.2137 g wheat bran,1.7843 g soybean meal,0.001 g MnSO·HO and 0.001 g CuSO·5HO in 10g dry matter fermentation medium.,Conclusions:After incubating 96 h in the optimum fermentation medium,alpha-galactosidase activity was predicted to be 2210·76 U gdry matter in 250 ml shake *** the present study,alpha-galactosidase activity reached 2207·19 U gdry matter. Significance and Impact of the Study:Optimization of the solid substrate was a very important measure to increase enzyme activity and realize industrial production of *** process of alpha-galactosidase production in laboratory scale may have the potential to scale-up.

关键词： alpha-galactosidase Aspergillus foetidus medium optimization response surface methodology solid-state fermentation.

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Optimization of the Fermentation Medium for alpha-galactosidase Production from Aspergillus foetidus ZU-G1 Using Response Surface Methodology

Optimization of the Fermentation Medium for Alpha-Galactosid...

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饲用酶制剂开发与应用技术交流研讨会

作者： Caiquin LIU Dept.of Food Science and Nutrition Zhejiang Univ.

The optimization of fermentation medium for alpha-galactosidase production by Aspergillus foetidus ZU-G1 was investigated in shaker flask fermentation.A one-factor-at-a-time experiment was used to screen the preferable nutriment(carbon sources,nitrogen sources,and essential elements) for alpha-galactosidase production. A fractional factorial design was used to screen the main 5 factors,soybean meal,wheat bran,KHPO,FeSO·7HO, and the medium initial pH,that affected the production of *** central composite experimental design was further adopted to derive a statistical model for optimizing the composition of the fermentation medium. The experimental results showed that the optimum fermentation medium for alpha-galactosidase production by Aspergillus foetidus ZU-G1 was composed of 3.2%soybean meal(w/v),2%wheat bran(w/v),0.1%KHPO(w/v), and 0.05%FeSO·7HO(w/v);initial medium pH was *** results further predicted that alpha-galactosidase activity reached 64.75 U/mL after 96-h incubation in this medium,which was approximately 7 times higher than that incubated in the nonoptimized *** time course of alpha-galactosidase production in the optimized medium composition was also carried out to validate themodel.

关键词： alpha-galactosidase Aspergillus foetidus ZU- G1 crude sources medium optimization response surface methodology

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Optimization of the Fermentation Medium for alpha-galactosidase Production from Aspergiilus foetidus ZU-G1 Using Response Surface Methodology

Optimization of the Fermentation Medium for Alpha-Galactosid...

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中国食品科学技术学会第五届年会暨第四届东西方食品业高层论坛

作者： Caiquin LIU Hui RUAN Huafeng SHEN Qihe CHEN Bing ZHOU Yi LI AND Guoqing HE (Authors Liu,Ruan,Shen,Chen,Zhou,and He are with Dept.of Food Science Nutrition,Zhejiang Univ.,Hangzhou,310029,China.Author Li is with Dept.of PlantSciences,NorthDakota StateUniv.,Fargo,ND58105,U.S.A.)

The optimization of fermentation medium for alpha-galactosidase production by Aspergillus foetidus ZU-Gl was investigated in shaker flask fermentation.A one-factor-at-a-time experiment was used to screen the preferable nutriment(carbon sources, nitrogensources,andessential elements)for alpha-galactosidase production.A fractional factorial design was used to screen the main 5 factors,soybean meal,wheat bran,KH2PO4,FeSO4·7H2O, and the medium initial pH,that affected the production of *** central composite experimental design was further adopted to derive a statistical model for optimizing the composition of the fermentation *** experimental results showed that the optimum fermentation medium for alpha-galactosidase production by Aspergillus foetidus ZU-Gl was composed of 3.2% soybean meal(w/v),2% wheat bran(w/v),0.1% KH2PO4(w/v),and 0.05% FeSO4.7H20(w/v);initial medium pH was *** results further predicted that alpha-galactosidase activity reached 64.75 U/mL after 95-h incubation in this medium,which was approximately 7 times higher than that incubated in the nonoptimized *** time course of alpha-galactosidase production in the optimized medium composition was also carried out to validate the model.

关键词： alpha-galactosidase Aspergillus foetidus ZU-G1 crude sources medium optimization response surface Methodology

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Response surface methodology for optimizing the fermentation medium of alpha-galactosidase in solid-state fermentation

Response surface methodology for optimizing the fermentation...

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饲用酶制剂开发与应用技术交流研讨会

作者： C.Q.Liu,Q.H.Chen,B.Tang,H.Ruan and G.Q.He Department of Food Science and Nutrition,Zhejiang University,Hangzhou 310029,China

Aims:alpha-galactosidase is applied in food and feed industries for hydrolysing raffinose series oligosaccharides(RO) that are the factors primarily responsible for flatulence upon ingestion of soybean-derived *** objective of the current work was to develop an optimal culture medium for the production of alpha-galactosidase in solid-state fermentation(SSF) by a mutant strain Aspergillus foetidus. Methods and Results:Response surface methodology(RSM) was applied to evaluate the effects of variables,namely the concentrations of wheat bran,soybean meal,KHPO,MnSO·HO and CuSO·5HO on alpha-galactosidase production in the solid substrate.A fractional factorial design(FFD) was firstly used to isolate the main factors that affected the production of alpha-galactosidase and the central composite experimental design(CCD) was then adopted to derive a statistical model for optimizing the composition of the fermentation *** experimental results showed that the optimum fermentation medium for alpha-galactosidase production by Aspergillus foetidus ZU-G1 was composed of 8-2137 g wheat bran,1·7843 g soybean meal,0·001 g MnSO·HO and 0·001 g CuSO·5HO in 10 g dry matter fermentation medium. Conclusions:After incubating 96 h in the optimum fermentation medium, alpha-galactosidase activity was predicted to be 2210·76 U g dry matter in 250 ml shake *** the present study,alpha-galactosidase activity reached 2207·19 U g dry matter. Significance and Impact of the Study:Optimization of the solid substrate was a very important measure to increase enzyme activity and realize industrial production of *** process of alpha-galactosidase production in laboratory scale may have the potential to scale-up.

关键词： alpha-galactosidase Aspergillus foetidus medium optimization response surface methodology solid-state fermentation

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