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Characterization of *** chromosome addition lines DA2H and MA5H

Journal of Genetics and Genomics 2008年第11期35卷 673-678页

作者： Fang Kong Haiyan Wang Aizhong Cao Bi Qin Jianhui Ji Suling Wang Xiu-E Wang State Key Laboratory of Crop Genetics and Germplasm Enhancement Cytogenetics Institute Nanjing Agricultural University Nanjing 210095 China

In order to transfer useful genes of Hordeum californicum into common wheat （Triticum aestivum L.）, the T. aestivum c.v. Chinese Spring （CS）-H. californicum amphiploid was crossed to CS, and its backcrossing and self-fertilized progenies were analyzed by morpho- logical observation, cytological, biochemical and molecular marker techniques. alien addition lines with two H. californicum chromo- somes were identified and their genetic constitution was characterized. STS-PCR analysis using chromosome 2B specific markers indi- cated that chromosome H3 of H. californicum belongs to homoeologous group 2, and was thus designated 2H. SDS-PAGE showed that chromosome H2 of H. californicum belongs to homoeologous group 5, and was designated 5H. The CS-H. californicum amphiploid and the chromosome addition lines （DA2H and MA5H） identified were evaluated for powdery mildew （Erysiphe graminis f. sp. triticii） resis- tance in field. The preliminary results indicated that the amphiploid showed higher powdery mildew resistance than CS. However, chro- mosome addition lines DA2H and MA5H were highly susceptible to powdery mildew, indicating that major powdery mildew resistant genes of H. californicum should be located on chromosomes other than 2H and 5H.

关键词： Hordeum californicum alien addition lines powdery mildew sequential C-banding/FISH sequence-tag-sites (STS)

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Molecular Cytogenetic Characterization of Wheat-Thinopyrum elongatum addition,Substitution and Translocation lines with a Novel Source of Resistance to Wheat Fusarium Head Blight

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Journal of Genetics and Genomics 2012年第2期39卷 103-110页

作者： Shulan Fu Zhenling Lv Bao Qi Xiang Guo Jun Li Bao Liu Fangpu Han State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics&Cytology Northeast Normal UniversityChangchun 130024China

Thinopyrum elongatum （2n = 2x = 14, EE）, a wild relative of wheat, has been suggested as a potentially novel source of resistance to several major wheat diseases including Fusarium Head Blight （FHB）. In this study, a series of wheat （cv. Chinese Spring, CS） substitution and ditelosomic lines, including Th. elongatum additions, were assessed for Type II resistance to FHB. Results indicated that the lines containing chromosome 7E of Th. elongatum gave a high level of resistance to FHB, wherein the infection did not spread beyond the inoculated floret. Furthermore, it was determined that the novel resistance gene（s） of 7E was located on the short-ann （7ES） based on sharp difference in FHB resistance between the two 7E ditelosomic lines for each arm. On the other hand, Th. elongatum chromosomes 5E and 6E likely contain gene（s） for susceptibility to FHB because the disease spreads rapidly within the inoculated spikes of these lines. Genomic in situ hybridization （GISH） analysis revealed that the alien chromosomes in the addition and substitution lines were intact, and the lines did not contain discernible genomic aberrations. GISH and multicolor-GISH analyses were further performed on three trans- location lines that also showed high levels of resistance to FHB. lines TA3499 and TA3695 were shown to contain one pair of wheat-Th. elongatum translocated chromosomes involving fragments of 7D plus a segment of the 7E, while line TA3493 was found to contain one pair of wheat-Th, elongatum translocated chromosomes involving the D- and A-genome chromosomes of wheat. Thus, this study has established that the short-arm of chromosome 7E of Th. elongatum harbors gene（s） highly resistant to the spreading of FHB, and chromatin of 7E introgressed into wheat chromosomes largely retained the resistance, implicating the feasibility of using these lines as novel material for breeding FHB-resistant wheat cultivars.

关键词： Thinopyrum elongatum alien addition lines Substitution lines Fusarium Head Blight resistance (FHB) Genomic in situ hybridization (GISH)

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Monosomic addition lines of Flowering Chinese Cabbage(*** *** *** ***)-Chinese Kale(*** *** ***)

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Agricultural Sciences in China 2008年第6期7卷 656-663页

作者： WANG Xin-e ZHANG Cheng-he XUAN Shu-xin MAN Hong LIU Hai-he SHEN Shu-xing College of Horticulture Agricultural University of Hebei Baoding 071001 P.R.China

Interspecific alien addition lines have played significant roles in gene mapping, intergenomic gene transfer and chromosomal homoeological identification between closely related species. Selection of alien addition lines was conducted by karyotype analysis and morphological observation with the reference of parents. Triploid interspecies hybrid （AAC, 2n = 3x = 29） was obtained from Brassica campestris ssp. chinensis var. parachinensis Qinglu 9601 （tetraploid, AAAA, 2n = 4x = 40） x B. oleracea var. alboglabra Baihua 9705 （diploid, CC, 2n = 2x = 18） by immature hybrid embryo culture in vitro. Five different alien monosomic addition lines （AA ＋ C2, AA ＋ C3, AA ＋ C4, AA ＋ C6, AA ＋ C7） were obtained from the backcross progenies of AAC x AA. Each alien monosomic addition line has some specific morphological characters. It is feasible to obtain alien addition lines from the progenies of AAC × AA by karyotype analysis and morphological observation based on the reference of parents

关键词： flowering Chinese cabbage Chinese kale allotriploid alien addition lines

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