检索结果-南通市图书馆

Journal of Environmental Sciences 2012年第10期24卷 1823-1831页

作者： Chuan Tian Xianglong Liu Jing Tan Shengqin Lin Daotang Li Hong Yang State Key Laboratory of Microbial metabolism School of Life Science and BiotechnologyShanghai Jiao Tong University800 Dongchuan Rd.Shanghai 200240China

In an effort to identify a bio-agent capable of controlling cyanobacterial blooms, we isolated a bacterial strain, A27, which exhibited strong algicidal activity against the dominant bloom-forming species of Microcystis aeruginosa in Lake Taihu. Based on 16S rRNA gene sequence analysis, this strain belongs to the genus Exiguobacterium. This is the first report of an algicidal bacterial strain belonging to the genus Exiguobacterium. Strain A27 exhibited algicidal activity against a broad range of cyanobacteria, but elicited little or no algicidal activity against the two green algal strains tested. The algicidal activity of strain A27 was shown to be dependent on the density of the bacteria and to have a threshold density of 1.5×106 CFU/mL. Our data also showed that the algicidal activity of strain A27 depended on different growth stages ofMicrocystis aeruginosa （exponential ≈ lag phase 〉 early stationary） rather than that of the bacterium itself. Our results also suggested the algicidal activity of strain A27 occurred via the production of extracellular algicidal compounds. Investigation of the algicidal compounds revealed that there were at least two different algicidal compounds produced by strain A27. These results indicated that strain A27 has great potential for use in the control of outbreaks of cyanobacterial blooms in Lake Taihu.

关键词： algicidal bacteria freshwater cyanobacteriat bloom Microcystis aeruginosa Exiguobacterium sp. A27

Isolation and algae-lysing characteristics of the algicidal bacterium B5

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2007年第11期19卷 1336-1340页

作者： MU Rui-min FAN Zheng-qiu PEI Hai-yan YUAN Xue-liang Liu Si-xiu WANG Xiang-rong Department of Environmental Science and Engineering Fudan University Shanghai 200433 China College of Environmental Science and Engineering Ocean University of China Qingdao 266003 China Environmental Research Institute Shandong University Jinan 250100 China

Water blooms have become a worldwide environmental problem. Recently, algicidal bacteria have attracted wide attention as possible agents for inhibiting algal water blooms. In this study, one strain of algicidal bacterium B5 was isolated from activated sludge. On the basis of analysis of its physiological characteristics and 16S rDNA gene sequence, it was identified as Bacillusfusiformis. Its algaelysing characteristics on Microcystis aeruginosa, Chlorella and Scenedesmus were tested. The results showed that：（1） the algicidal bacterium B5 is a Gram-negative bacterium. The 16S rDNA nucleotide sequence homology of strain B5 with 2 strains of B. fusiformis reached 99.86%, so B5 was identified as B. fusiformis; （2） the algal-lysing effects of the algicidal bacterium B5 on M. aeruginosa, Chlorella and Scenedesrnus were pronounced. The initial bacterial and algal cell densities strongly influence the removal rates of chlorophyll-a. The greater the initial bacterial cell density, the faster the degradation of chlorophyll-a. The greater the initial algal cell density, the slower the degradation of chlorophyll-a. When the bacterial cell density was 3.6 × 10^7 cells/ml, nearly 90% of chlorophyll-a was removed. When the chlorophyll-a concentration was less than 550 μg/L, about 70% was removed; （3） the strain B5 lysed algae by secreting metabolites and these metabolites could bear heat treatment.

关键词： algicidal bacteria water blooms algae-lysing characteristic 16S rDNA Bacillusfusiformis

Microbial community coexisting with harmful alga Karenia mikimotoi and microbial control of algal bloom in laboratory

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2022年第3期40卷 1027-1038页

作者： Li SUN Peike GAO Yu LI Chao WANG Ning DING Junfeng CHEN Yuhao SONG Chunchen LIU Lun SONG Renjun WANG College of Life Sciences Qufu Normal UniversityQufu 273165China Key Laboratory of Marine Biological Resources and Ecology Liaoning Ocean and Fisheries Science Research InstituteDalian 116023China

algicidal bacteria have been frequently isolated from algal blooming ***,knowledge regarding the microbial communities coexisting with microalgae and their potential application in preventing harmful algal blooms(HABs)is *** this study,we investigated the composition of the microbial community coexisting with harmful alga Karenia mikimotoi and its responses to algal control via nutrient stimulation or by adding algicidal strain in *** microorganisms inhabiting the *** culture consisted of 24 identifi ed phyla,including dominant Proteobacteria(relative abundance 76.24%±7.28%)and Bacteroidetes(22.67%±8.32%).Rhodobacteraceae,Phaeodactylibacter,and Maritimibacter predominated during the algal *** the added nutrient and fermentation broth of algicidal strain Pseudoalteromonas QF1 caused a massive death of *** and substantial changes in the coexisting microbial community,in which Rhodobacteraceae and Phaeodactylibacter signifi cantly decreased,while Halomonas and Alteromonas *** operational taxonomic units(OTUs)analysis indicated that 13 OTUs belonging to Rhodobacteraceae,Maritimibacter,Marivita,Nisaea,Phaeodactylibacter,Citreicella,Halomonas,Alteromonas,Marinobacter,Muricauda,and Pseudoalteromonas dominated the changes of the microbial communities observed in the *** culture with or without ***,this study indicated that microbial community inhabiting *** culture includes potential algicidal bacteria,and improves our knowledge about microbial community succession during biocontrol of *** via nutrient stimulation or by adding isolated algicidal strains.

关键词： Karenia mikimotoi microbial community nutrient stimulation algicidal bacteria Pseudoalteromonas

Optimization of Culture Conditions and Medium Composition for the Marine algicidal Bacterium Alteromonas sp. DH46 by Uniform Design

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2013年第3期12卷 385-391页

作者： LIN Jing ZHENG Wei TIAN Yun WANG Guizhong ZHENG Tianling State Key Laboratory of Marine Environmental Science and Key Laboratory of the Ministry of Education for Coast and Wetland Ecosystems School of Life SciencesXiamen University

Harmful algal blooms(HABs) have led to extensive ecological and environmental issues and huge economic *** HAB control techniques have been developed,and biological methods have been paid more *** bacteria is a general designation for bacteria which inhibit algal growth in a direct or indirect manner,and kill or damage the algal cells.A metabolite which is strongly toxic to the dinoflagellate Alexandrium tamarense was produced by strain DH46 of the alga-lysing bacterium Alteromonas *** culture conditions were optimized using a single-factor test *** including carbon source,nitrogen source,temperature,initial pH value,rotational speed and salinity were *** results showed that the cultivation of the bacteria at 28℃ and 180 r min-1with initial pH 7 and 30 salt contcentration favored both the cell growth and the lysing effect of strain *** optimal medium composition for strain DH46 was determined by means of uniform design experimentation,and the most important components influencing the cell density were tryptone,yeast extract,soluble starch,NaNO3 and *** the following culture medium was used(tryptone 14.0g,yeast extract 1.63g,soluble starch 5.0 g,NaNO3 1.6 g,MgSO4 2.3 g in 1L),the largest bacterial dry weight(7.36 g L-1) was obtained,which was an enhancement of 107% compared to the initial medium;and the algal lysis rate was as high as 98.4% which increased nearly 10% after optimization.

关键词： algicidal bacteria optimization culture conditions medium composition uniform design

algicidal effect of bacterial isolates of Pedobacter sp. against cyanobacterium Microcystis aeruginosa

在线全文

学校读者我要写书评

暂无评论

Water Science and Engineering 2012年第4期5卷 375-382页

作者： Li YANG Hiroto MAEDA Takeshi YOSHIKAWA Gui-qin ZHOU College of Environment Hohai University College of Environment Nanjing University of Technology Laboratory of Microbiology Faculty of FisheriesKagoshima University

The aim of this study was to isolate algicidal bacteria so as to control harmful cyanobacterium Microcystis aeruginosa （M. aeruginosa） blooms using biological methods. Nine bacterial strains were isolated to inhibit the growth ofM. aeruginosa, among which the Mail 1-5 bacterial strain exhibited remarkable algicidal activity against M. aeruginosa cells during the test. Based on the 16S rDNA analysis, the isolated Mail 1-5 was identified as Pedobacter sp. through morphology and homology research. The results of cocultivation of the cyanobacteria with MaI 11-5 algicidal isolates showed obvious algicidal activity against cyanobacterial cells. The algicidal effect of Mail 1-5 exceeded 50% after two days, exceeded 70% after four days, and reached 80% after seven days. The observation results with a scanning electron microscope showed that the cyanobacterial cells aggregated and produced mucous-like substances when cocultivated with the algicidal bacteria. The results indicated that the Mail 1-5 bacterial strain may possess a novel function for controlling harmful blooms and further studies will provide new insights into its role in water environment.

关键词： algicidal bacteria Pedobacter sp. algicidal effect Microcystis aeruginosa

Isolation and Characterization of an algicidal Bacterium Indigenous to Lake Taihu with a Red Pigment able to Lyse Microcystis aeruginosa

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2013年第2期26卷 148-154页

作者： YANG Fei WEI Hai Yan LI Xiao Qin LI Yun Hui LI Xiao Bo YIN Li Hong PU Yue Pu Key Laboratory of Environmental Medicine Engineering Ministry of EducationSchool of Public HealthSoutheast University

Objective To isolate and characterize indigenous algicidal bacteria and their algae-lysing compounds active against Microcystis aeruginosa, strains TH1, TH2, and FACHB 905. Methods The bacteria were identified using the Biolog automated microbial identification system and 16S rDNA sequence analysis. The algae-lysing compounds were isolated and purified by silica gel column chromatography and reverse-phase high performance liquid chromatography. Their structures were confirmed by Nuclear Magnetic Resonance （NMR） and Fourier Transform infrared （FT-IR） spectroscopy. Algae-lysing activity was observed using microscopy. Results The algae-lysing bacterium LTH-2 isolated from Lake Taihu was identified as Serratia marcescens. Strain LTH-2 secreted a red pigment identified as prodigiosin （C20H25N30）, which showed strong lytic activity with algal strains M. aeruginoso TH1, TH2, and FACHB 905 in a concentration-dependent manner. The 50% inhibitory concentration （ICs0） of prodigiosin with the algal strains was 4.8 （±0.4）×10^-2 μg/mL, 8.9 （±1.1）×10^-2μg/mL, and 1.7 （±0.1）×10^-1 μg/mL in 24 h, respectively. Conclusion The bacterium LTH-2 and its pigment related to damage of cell membranes. The bacterium for regulating blooms of harmful M. aeruginosa. had strong Microcystis-lysing activity probably LTH-2 and its red pigment are potentially useful

关键词： algicidal bacteria Microcystis aeruginosa Harmful algal blooms (HABs) Serratiamarcescens Algae-lysing

Dynamic Analysis of an Algae-bacteria Ecological Model

在线全文

学校读者我要写书评

暂无评论

Journal of Applied Mathematics and Physics 2024年第1期12卷 362-382页

作者： Gaopeng Sun Hengguo Yu School of Mathematics and Physics Wenzhou University Wenzhou China

In the paper, under the framework of exploring the interaction between algae and bacteria, an algae-bacteria ecological model was established to analyze the interaction mechanism and growth coexistence mode between algicidal bacteria and algae. Firstly, mathematical work mainly provided some threshold conditions to ensure the occurrence of transcritical bifurcation and saddle-node bifurcation, which could provide certain theoretical support for selecting key ecological environmental factors and numerical simulations. Secondly, the numerical simulation work dynamically displayed the evolution process of the bifurcation dynamic behavior of the model (2.1) and the growth coexistence mode of algae and algicidal bacteria. Finally, it was worth summarizing that intrinsic growth rate and combined capture effort of algae population had a strong influence on the dynamic behavior of the model (2.1). Furthermore, it must also be noted that transcritical bifurcation and saddle-node bifurcation were the inherent driving forces behind the formation of steady-state growth coexistence mode between algicidal bacteria and algae. In summary, it was hoped that the results of this study would contribute to accelerating the study of the interaction mechanism between algicidal bacteria and algae.

关键词： Algae algicidal bacteria Transcritical Bifurcation Saddle-Node Bifurcation Coexistence Mode