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The biotechnological potential of using mono-and co-cultures of Aspergillus niger van Tieghem and Trichoderma viride Pers ex *** enhance the protein content of cassava(Manihot esculenta Crantz)peels by solid substrate fermentation

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Studies in Fungi 2019年第1期4卷 111-122页

作者： Yafetto L Odamtten GT Adu SK Ekloh E Department of Molecular Biology and Biotechnology School of Biological SciencesCollege of Agriculture and Natural SciencesUniversity of Cape CoastCape CoastGhana Department of Plant and Environmental Biology School of Biological SciencesCollege of Basic and Applied SciencesUniversity of GhanaLegon-AccraGhana Department of Animal Science School of Agricultural SciencesCollege of Agriculture and Natural SciencesUniversity of Cape CoastCape CoastGhana

The application of fungal biotechnology for nutrient value addition and improvement of agro-industrial wastes is critical in the development of protein-rich feed for *** study evaluated conventional methods for protein enrichment of cassava peels by solid substrate fermentation using mono-and co-cultures of Aspergillus niger and Trichoderma *** and non-sterile cassava peels inoculated with mono-cultures of *** or *** and co-cultures of *** and *** were spontaneously fermented for 21 days at 24℃.Fermented substrates were harvested at 0,7,14,and 21 days intervals,then oven-dried at 60℃,milled in a blender and further assayed for ash,carbohydrate,crude fibre,fat,and protein *** showed that percentage increase in protein contents after 21 days incubation of sterile cassava peels were 71.02%(for mono-culture of ***)and 71.64%(for mono-culture of ***);co-culture of *** and *** yielded a 129.00%increase in *** percentage increase in protein content of the non-sterile cassava peels was 126.80%(for mono-culture of ***)and 168.34%(for mono-culture of ***)in contrast with 63.47%obtained for the co-culture of *** and *** in non-sterile cassava peels,which was attributable to possible antibiosis due to microbial *** content significantly(p≤0.05)increased for 21 days while carbohydrate,crude fibre and fat contents in both sterile and non-sterile cassava peels decreased over the same *** findings underscore the fact that protein content of cassava peels can be significantly(p≤0.05)enriched through fungal biotechnology to produce value-added feed supplement for *** practical implications of these findings are discussed.

关键词： agro-industrial wastes environmental mycoremediation fungal biotechnology nutrient-enriched feed

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Evaluation of sources and methods of pectin extraction from fruit and Vegetable wastes:A Systematic Literature Review(SLR)

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Food Bioscience 2023年第1期51卷 1370-1379页

作者： Carolina Sofía Salazar Ripoll Gustavo Adolfo Hincapie-Llanos Universidad Pontificia Bolivariana MedellínAntioquiaColombia

The potentially commercial sources of pectin are *** have studied different extraction methods using a wide variety of fruits and vegetables to obtain pectins with better *** objective of this work was to identify the methods used in the extraction of pectin,the most used sources and the operation parameters that enable the achievement of higher yields and the final characteristics of the obtained *** are based on bibliometric methods for exploring the structure of knowledge in this field for the last twelve *** this end,a Systematic Literature Review(SLR)using the SCOPUS database was carried out,which allowed identifying keywords and formulating search equations to find scientific articles on the topic in *** selecting the articles,a detailed reading of these works was carried out,and the used extraction methods and the evaluated variables,yields and characteristics were *** article compares and discusses the effects of conventional and non-conventional pectin extraction techniques,which may provide valuable information on sources and alternatives for pectin extraction to ease decision-making in future *** acid hydrolysis method remains the most widely used pectin extraction technique since it provides higher *** is followed by microwave-assisted extraction,ultrasonic extraction,and the enzymatic method,which have a greater capacity to deliver pectins with high methoxyl ***,non-conventional methods such as radiofrequency,ohmic heating,and aqueous two-phase extraction are presented as promising techniques that offer pectins with a high degree of esterification in addition to good yields.

关键词： agro-industrial wastes Acid hydrolysis Microwave Ultrasound Degree of esterification Galacturonic acid

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Protein enrichment of cassava pulp by solid-state fermentation using Aspergillus niger

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Studies in Fungi 2018年第1期3卷 7-18页

作者： Yafetto L Department of Molecular Biology and Biotechnology School of Biological SciencesCollege of Agriculture and Natural SciencesUniversity of Cape CoastCape CoastGhana

This study aimed to assess protein enrichment of sterile and non-sterile cassava pulp using four strains of Aspergillus niger-AN1,AN2,AN3,***,studies were conducted to evaluate nutritional requirements of *** strains and their suitability for protein enrichment of cassava ***,sterile and non-sterile cassava pulps were inoculated with spores of *** strains and incubated under conditions of solid-state fermentation for 8 days using standard *** contents of sterile and non-sterile cassava pulps were determined by the Kjeldahl *** nutritional requirements studies showed that *** strains grew at rates between 2.0 cm and 8.5 cm over 10 days on potato dextrose agar medium of different *** dry weight of mycelia of *** strains varied in liquid media separately supplemented with different concentrations of thiamine,ammonium nitrate and sodium *** strains also showed different levels of sporulation when cultured in growth media supplemented with different concentrations of sodium *** in protein contents of sterile cassava pulp by AN1,AN2,AN3 and AN4 were 15.65%,22.61%,18.30%and 19.13%,*** non-sterile cassava pulp,the increases in protein content by AN1,AN2,AN3 and AN4 were 15.40%,21.54%,10.80%and 3.85%,*** strain AN2 was the most suitable strain for the enrichment of protein content of cassava because it sparsely produced spores,it had the highest mycelial growth and resulted in the greatest increase in protein content of both sterile and non-sterile cassava pulp.

关键词： agro-industrial wastes livestock feed nutrient improvement solid-substrate fermentation

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