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Association between delayed cardioprotection of aged rat myocytes and activation of mitogenactivated protein kinase

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Chinese Medical Journal 2000年第1期113卷 5-9页

作者：刘秀华王士雯武旭东唐朝枢 Institute of Geriatric Cardiology Chinese PLA General Hospital Beijing 100853 China.

Objective To study the cardioprotective effects of hypoxic preconditioning (HPC) on aged rat ventricular myocytes and the cellular mechanism of protection Methods In the model of hypoxia/reoxygenation (H/R) of isolated ventricular myocytes of aged rat, the effects of HPC on aged rat ventricular myocytes against lethal H/R stimulated ischemia/reperfusion (I/R) injury 24 hours later and the changes of mitogen activated protein kinase (MAPK) system were observed in the present study Results HPC attenuated the lactate dehydrogenase (LDH) release and ATP depletion in myocytes and increased the viability of myocytes It was also found that MAPK and its down stream kinase—S6 kinase were also activated after HPC Conclusion There is delayed cardioprotection in cardiac myocytes of aged rat and the cellular mechanism underlying might involve the activation of MAPK system

关键词： hypoxic preconditioning delayed cardioprotection mitogen activated protein kinase aged rat

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Effects of Herba Epimedii and Fructus Ligustri lucidi on the Transcription Factors in Hypothalamus of aged rats

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Chinese Journal of Integrative Medicine 2011年第10期17卷 758-763页

作者：蔡晶郑腾张磊田允杨民和杜建 Fujian Institute of Integrative Chinese and Western Medicine Fujian University of Traditional Chinese Medicine Fujian Entry-Exit Inspection and Quarantine Bureau of P.R.China Fujian Normal University

Objective： To comparatively analyze the difference in the expression of 54 transcription factors in the hypothalamus using protein chips following the medication of Chinese drugs for Shen （肾）-tonification, Herba Epimedii, and Fructus Ligustri lucidi （FL） to aged rats. Methods： Wistar rats, aged 15 months of SPF grade, were randomized into three groups, three males and three females in each group. They were medicated with Herba Epimedii decoction （HED, 0.14 g/kg）, Fructus Ligustri lucidi decoction （FLD, 0.12 g/kg）, and distilled water, respectively, twice a day for 15 days. The rats were sacrificed at the morning of the 16th day 1 h after medication, and their hypothalamus was taken and made into homogenate under an ice-bath for detecting the expression of transcription factors with chip technique. Results： The expressions of signal transduction and transcription activation factor-6 （Stat-6） and androgen receptor （AR） were up-regulated, and those of pre-B transcription factor1 （Pbx-1）, stat-1 and AP-2 were down-regulated in both HED and FLD treated groups, but these changes occurred mainly in female rats in the former while mainly in males in the latter. Conclusions： Chinese drugs for Shen-tonification could impact the expression of transcription factors in the hypothalamus of aged rats, dominantly on the neuro-endocrine factors responsible for the growth and development. The effects of drugs for tonifying Shen-yang and for tonifying Shen-yin are different, which is probably one of the pharmacological mechanisms of the Shen-tonifying drugs.

关键词： Herba Epimedii Fructus Ligustri lucidi transcription factor aged rat hypothalamus

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Influence of ginsenoside on expression of brain-derived neurotrophic factor and receptor tyrosine kinase B in the medial septum of aged rats

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Neural Regeneration Research 2008年第5期3卷 490-493页

作者： Liang Zeng Haihua Zhao Yongli Lu Wenbo Dai Department of Anatomy Shenyang Medical College Shenyang 110034 Liaoning Province China Department of Anatomy China Medical University Shenyang 110001 Liaoning Province China Department of Pathology First People＇s Hospital Shenyang 110041 Liaoning Province China

BACKGROUND： It has beenshown that ginsenoside, the effective component of ginseng, can enhance expression of choline acetyl transferase, as well as brain-derived neurotrophic factor （BDNF） and its receptor tyrosine kinase B （TrkB）, in cholinergic neurons of the basal forebrain. OBJECTIVE： To qualitatively and quantitatively verify the influence of ginsenoside on expression of BDNF and its receptor, TrkB, in the medial septum of aged rats, and to provide a molecular basis for clinical application. DESIGN~ TIME AND SETTING： A contrast study, which was performed in the Department of Anatomy, China Medical University, and the Department of Anatomy, Shenyang Medical College between December 2005 and May 2007. MATERIALS： Thirty-five, healthy, female, Sprague Dawley rats were selected for this study. Ginsenoside （81% purity） was provided by Jilin Ji＇an Wantai Chinese Medicine Factory; anti-BDNF antibody, anti-TrkB antibody, and their kits were provided by Wuhan Boster Company. METHODS： A total of 35 rats were divided into three groups： young （four months old）, aging （26 months old）, and ginsenoside. rats in the ginsenoside group were administered ginsenoside （25 mg/kg/d） between 17 months and 26 months. MAIN OUTCOME MEASURES： Immunohistochemistry and in situ hybridization were used to measure expression of BDNF and TrkB in the medial septum of aged rats, and the detected results were expressed as gray values. RESULTS：（1） Qualitative detection： using microscopy, degenerative neurons were visible in the medial septum in the aging group. However, neuronal morphology in the ginsenoside group was similar to neurons in the young group. （2） Quantitative detection： the mean gray value of BDNF-positive and TrkB-positive products in the aging group were significantly higher than in the young group （t = 3.346, 4.169, P 〈 0.01）; however, the mean gray value in the ginsenoside group was significantly lower than in the aging group （t = 2.432, 2.651, P 〈 0.01）. CONCLUSION： Ginsenoside c

关键词： BDNF TrkB ginsenoside medial septum aged rat

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The Changes of MicroRNAs-Fkbps-GR Regulatory Circuits in Hippocampus of aged rats and the Adjustment Effect of Kidney-Tonifying and Yi Qi Recipe

The Changes of MicroRNAs-Fkbps-GR Regulatory Circuits in Hip...

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中国生物化学与分子生物学会2019年全国学术会议暨学会成立四十周年

作者： Wang Lu Xiang-Ping Kang Xue-Li Zhang Cui-Ying Gu Guo-Qin Jin Department of Biochemistry School of Basic Medicine Shanghai University of Traditional Chinese Medicine

The 5 months male SD rats as the young control group, 23 months homologous strain rat are randomly divided into aged control group、aged Tonifying Kidney(Zuogui Pill) group, aged Yi Qi(Yiqi Smart Decoction)group, aged mixture(TK+YQ) group and aged antagonists(RU486) group. The main purpose of the project is to observe microRNAs-Fkbps-GR regulatory circuits affect GR nuclear translocation in aged rats and its effect on learning and memory related genes expression and the adjustment effect of Tonifying Kidney and Yi Qi recipe. The spatial memory ability of rats was observed by Morris water maze method. MicroRNAs-Fkbps-GR Regulatory Circuits(GC, Fkbp5/4, Dynein IC2, GR, miR-124 a/511) and learning memory related signal transduction molecules(Nptx2, Synapsin-1, Homer1) expression by biochemistry and molecular biology technology. The results showed that: Compared with the young control group, the spatial learning and memory ability of the aged rats was significantly decreased, and the serum corticosterone content was significantly increased. The expression of total GR protein and nuclear GR protein in hippocampus was significantly decreased, the expression in cytoplasmic GR protein was significantly increased, and the expression of GR mRNA was significantly decreased. The expression of Dynein IC2 was significantly decreased, the expression of Fkbp5 and Fkbp4 protein was significantly increased, and the expression of Fkbp5 mRNA was significantly increased. miR-511 expression was significantly decreased,miR-124 a expression was significantly increased. The expression of learning and memory related genes Nptx2 and Synapsin-1 gene were significantly decreased, and the expression of Homer1 gene was not significantly changed. The medication groups(TK,YQ,TK+YQ and RU486)have different corrective effects on the abnormal changes of the above-mentioned indexes in the aged rats. Thus it can be seen, Tonifying Kidney(Zuogui Pill) and Yiqi Smart Decoction mainly reduce the serum GC co

关键词： aged rat glucocorticoid learning and memory Tonifying Kidney and Yiqi Recipe

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