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Protein & Cell 2024年第6期15卷 403-418页

作者： Song Xue Yuru Han Fan Wu Qiao Wang Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS) Shanghai Institute of Infectious Disease and BiosecurityShanghai Frontiers Science Center of Pathogenic Microorganisms and InfectionSchool of Basic Medical SciencesShanghai Medical CollegeFudan UniversityShanghai 200032China

Intensive selection pressure constrains the evolutionary trajectory of SARS-CoV-2 genomes and results in various novel variants with distinct mutation *** mutations,particularly those within the receptor binding domain(RBD)of SARS-CoV-2 spike(S)protein,lead to the functional alteration in both receptor engagement and monoclonal antibody(mAb)***,we review the data of the RBD point mutations possessed by major SARS-CoV-2 variants and discuss their individual effects on ACE2 affinity and immune *** single amino acid substitutions within RBD epitopes crucial for the antibody evasion capacity may conversely weaken ACE2 binding ***,this weakened effect could be largely compensated by specific epistatic mutations,such as N501Y,thus maintaining the overall ACE2 affinity for the spike protein of all major *** predominant direction of SARS-CoV-2 evolution lies neither in promoting ACE2 affinity nor evading mAb neutralization but in maintaining a delicate balance between these two ***,this review interprets how RBD mutations efficiently resist antibody neutralization and meanwhile how the affinity between ACE2 and spike protein is maintained,emphasizing the significance of comprehensive assessment of spike mutations.

关键词： ACE2 affinity maintained

多体非完全经典态的基于affinity的关联测度

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太原科技大学学报 2024年第1期45卷 101-103,108页

作者：闫栋华王银珠太原科技大学应用科学学院太原030024

在量子信息理论中,量子关联是一种极其重要的物理资源。文章首先定义了多体非完全经典态基于affinity的一种量子关联测度,它可以量化多体非完全经典态的关联程度;接下来证明了该关联测度满足量子关联度的一些必要物理性质,包括非负性、... 详细信息

在量子信息理论中,量子关联是一种极其重要的物理资源。文章首先定义了多体非完全经典态基于affinity的一种量子关联测度,它可以量化多体非完全经典态的关联程度;接下来证明了该关联测度满足量子关联度的一些必要物理性质,包括非负性、局部酉操作下的不变性、局部量子操作下的单调性、凸性以及连续性。

关键词：多体复合系统完全经典态投影测量量子关联度 affinity

Universal chimeric Fcγreceptor T cells with appropriate affinity for IgG1 antibody exhibit optimal antitumor efficacy

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Acta Pharmaceutica Sinica B 2023年第5期13卷 2071-2085页

作者： Wen Zhu Yang Wang Liangyin Lv Hui Wang Wenqiang Shi Zexin Liu Mingzhe Zhou Jianwei Zhu Huili Lu Engineering Research Center of Cell and Therapeutic Antibody Ministry of EducationSchool of PharmacyShanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Genetic Engineering School of Life ScienceFudan UniversityShanghai 200433China

Developing universal CARs with improved flexible targeting and controllable activities is urgently *** several studies have suggested the potential of CD16a in tandem with monoclonal antibodies to construct universal CAR-T cells,the weak affinity between them is one of the limiting factors for ***,we systematically investigated the impact of Fcγreceptor(FcγR)affinity on CAR-T cells properties by constructing universal CARs using Fcγreceptors with different affinities for IgG1 antibodies,namely CD16a,CD32a,and *** demonstrated that the activities of these universal CAR-T cells on tumor cells could be redirected and regulated by IgG1 *** xenografted mice,64CAR chimeric Jurkat cells with the highest affinity showed significant antitumor effects in combination with herceptin in the HER2 low expression U251 MG ***,in the CD20 high expression Raji model,64CAR caused excessive activation of CAR-T cells,which resulted in cytokine release syndrome(CRS)and the decline of antitumor activity,and 32CAR with a moderate affinity brought the best *** work extended the knowledge about FcγR-based universal CAR-T cells and suggested that only the FcγRCAR with an appropriate affinity can offer the optimal antitumor advantages of CAR-T cells.

关键词： Universal CAR-T cells Fcγreceptor CD16a CD32a CD64 affinity IgG1 antibody CRS

Characterization of folic acid,5-methyltetrahydrofolate and synthetic folinic acid in the high-affinity folate transporters:impact on pregnancy and development

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Reproductive and Developmental Medicine 2023年第2期7卷 102-107页

作者： Ana M.Palacios Rachel A.Feiner Robert M.Cabrera Department of Nutritional Sciences Dell Pediatric Research InstituteUniversity of Texas at AustinAustinTexas Department of Health Policy and Community Health Jiann Ping Hsu College of Public HealthGeorgia Southern UniversitySavannahGeorgia Department of Behavioral Neuroscience College of ScienceNortheastern UniversityBostonMassachusetts Center for Precision Environmental Health Baylor College of MedicineHoustonTexas

Objectives:Folates are B vitamins that are essential for several molecular,cellular,and biological processes,including nucleotide synthesis,methylation,and methionine *** physiological impacts of these processes on health also extend to cell proliferation,folate deficiency anemia,and reduction of the risk of birth defects during *** primary objective of this study was to characterize the binding affinities of different folate forms,folic acid(FA),5-methyltetrahydrofolate(5MTHF),and folinic acid,to the folate receptorsαandβ,and to the bovine milk folate binding *** three dietary forms of folate are found in enriched grains(FA),various fruits and leafy vegetables(folinic acid),and red blood cells(5MTHF).Methods:The half maximal inhibitory concentration values and binding curves of each of these folates for each receptor were ***:Our results indicated that FA had the highest affinity for all folate receptors,followed by 5MTHF,and lastly,by folinic acid,examined by several orders of ***:These data are expected to provide new insights into the therapeutic applications of the different forms of folate in a variety of diseases.

关键词： 5-methyltetrahydrofolate folic acid folinic acid folate receptorα folate receptorβ affinity

affinity and fluorescent detection of surfactants/ssDNA and single-walled carbon nanotube

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Transactions of Nonferrous Metals Society of China 2013年第2期23卷 456-461页

作者：周姣李娟萍聂钰洪李继山杨金凤湖南大学化学化工学院化学生物传感与计量学国家重点实验室长沙410082 中南大学湘雅医学院附属肿瘤医院麻醉科长沙410013

A new biosensor platform was explored for detection of surfactant based on fluorescence changes from single strand DNA （ssDNA） and single-walled carbon nanotubes （SWNTs）. Thermodynamics assay was performed to value the stability of probe. The affinities of SWNT to five common surfactants （SDS, DBS, Triton X-100, Tween-20 and Tween-80） were investigated by real-time fluorescence method. The effects of Mg^2＋ and pH on the fluorescence intensity of self-assembled quenched sensor were performed. The fluorescent emission spectra were used to measure the responses of self-assembled quenched fluorescent of ssDNA/SWNTs to different concentration surfactant（Triton X-100）. The FAM-DNA wrapped SWNTs probe was stable in a wide temperature range （5 ℃ to 80℃）. The binding strength of surfactants and single-stranded DNA （ssDNA） on SWNTs surfaces was shown as follows： Triton X-100〉DBS〉Tween-20〉Tween-80〉ssDNA〉SDS, and the optimized reaction conditions included pH 7.4 and 10 mmol/L Mg2＋. The fluorescence of FAM-ssDNA wrapped SWNTs was proportionally recovered as a result of adding different concentrations of Triton X- 100, which realizes the quantitative detection of Triton X- 100.

关键词： single-stranded DNA single-walled carbon nanotubes surfactant fluorescent sensor affinity

affinity CHROMATOGRAPHY PURIFICATION OF UROKINASE WITH EPICHLOROHYDRIN ACTIVATED AGAROSE MATRIX

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Chinese Journal of Chemical Engineering 1997年第1期5卷 73-77页

作者：曹学君朱家文王大为戴干策邬行彦华东理工大学化学工程研究中心上海华东理工大学生物化学工程系上海

1 INTRODUCTIONIn literature,most matrices of affinity chromatography for urokinase(EC 3.***.99.26)purification were prepared by cyanogen bromide ***,theseadsorbents usually suffered from the drawback of leakage of the ligand,particularly inalkaline medium,because of the instability of the isourea linkage between the ligandand the spacer or ***,the positively charged imido group of theN-substituted isourea derivative and the hydrophobicity of the spacers might promotenonspecific *** the contrary,the adsorbents prepared by the method

关键词： epichlorohydrin agarose p-aminobenzamidine urokinase affinity chromatography

affinity ultrafiltration of DNA topoisomerases-targeted compounds determined with HPLC／ESI-MS for drug candidate screening

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Journal of Zhejiang University Science 2004年第8期5卷 900-905页

作者：张虹潘远江 DepartmentofChemistry ZhejiangUniversityHangzhou310027China

A method of screening assay is demonstrated. The approach is based on the affinity of antitumor candidates for topoisomerases. In this method, antitumor candidates are fished out using topoisomerases as targets. Traditional analysis of complex compounds typically encounters signal suppression due to the relatively low concentrations, but enzyme-affinity screening for the active compounds can effectively concentrate the desired analysts into a small volume of high concen-tration. Active compounds are separated from non-affinity compounds by ultrafiltration. The molecules-enzymes complexes that are retained on the filter are subsequently separated by acidification to obtain the topoisomerases-affinity compounds for analysis on High Performance Liquid Chromatography coupled with electrospray ionization mass spectrometric detec-tion (ESI-MS). This enzyme-affinity based screening assay provides a highly specific and efficient method that can directly screen, identify, and acquire drug candidates thus improving the accuracy and speed of high-throughput screening activities.

关键词： affinity High-throughput screening LC-MS Topoisomerase Ultrafiltration

affinity between Palaeozoic Blocks of Xinjiang and Their Suturing Ages

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Acta Geologica Sinica(English Edition) 2000年第1期74卷 1-6页

作者： GUO Fuxiang Research Centre of Prediction of Hidden Ore Deposits Guilin Institute of Technology Guilin 541004

Abstract: The Kazakhstanian plate was near the Tarim, Sino-Korean and South China-Southeast Asian plates in the Middle-Late Cambrian and Late Ordovician, and approached the Siberian plate in the other periods of the Early Palaeozoic. The Ili and Toksun-Yamansu terranes had been split from the Tarim plate before the Middle Devonian and then went close to Angaraland in the late Early Permian. The Beishan area on the northeastern margin of the Tarim plate came close to Angaraland first in the early Early Permian. The suturing age between the Tarim and Kazakhstanian-Siberian plates is generally the same as that between the Turkey-Central Iran-Gangdise and South China-Southeast Asian plates. The suturing event took place in the early Early Permian, while the corresponding tectogeny occurred between the Early and Late Permian.

关键词： plate terrane affinity suturing age Xinjiang

Accurate calculation of electron affinity for S_3

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Chinese Physics B 2019年第1期28卷 348-352页

作者： Xue Yang Haifeng Xu Bing Yan College of Science Jilin Institute of Chemical Technology Jilin Provincial Key Laboratory of Applied Atomic and Molecular Spectroscopy Institute of Atomic and Molecular PhysicsJilin University

The accurate equilibrium structures of S_3 and S_3^- are determined by the coupled-cluster method with single, double excitation and perturbative triple excitation(CCSD(T)) with basis sets of aug-cc-pV(n+d)Z(n = T, Q, 5, or 6), complete basis set extrapolation functions with two-parameters and three-parameters, together with considering the contributions due to the core-valence electron correlation, scalar relativistic effects, spin–orbit coupling, and zero-point vibrational corrections. Our calculations show that both the neutral S_3 and anion S_3^- have open forms with C_(2r) vsymmetry. On the basis of the stable geometries, the adiabatic electron affinity of S_3 is determined to be 19041(11) cm^(-1), which is in excellent agreement with the experimental data(19059(7) cm^(-1)). The dependence of geometries and electron affinity on the computation level and physical corrections is discussed. The present computational results are helpful to the experimental molecular spectroscopy and bonding of S_3.

关键词： S3 molecular geometry adiabatic electron affinity coupled-cluster method with single double excitation and perturbative triple excitation(CCSD(T))

Nano CuO/g-C_3N_4 sheets-based ultrafiltration membrane with enhanced interfacial affinity,antifouling and protein separation performances for water treatment application

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Journal of Environmental Sciences 2019年第8期31卷 57-69页

作者： Thanigaivelan Arumugham Reshika Gnanamoorthi Amimodu Noel Jacob Kaleekkal Dipak Rana Department of Chemistry School of Advanced Sciences Vellore Institute of Technology (VIT) Department of Integrative Biology School of Bioscience and Technology Vellore Institute of Technology (VIT) Department of Chemical Engineering National Institute of Technology Calicut Department of Chemical and Biological Engineering Industrial Membrane Research Institute University of Ottawa

To improve the interfacial affinity and antifouling properties of polyphenylsulfone(PPSU)membrane, nano CuO/g-C3 N4(g-CN) sheets were synthesized via facile calcination route as one pot synthesis method. The uniformly assembled nanohybrid fillers, CuO on g-CN sheets were confirmed by using XRD, TEM, EDX and FTIR analysis. The non-solvent induced phase inversion technique was used to fabricate the nanohybrid ultrafiltration(UF)membranes by doping different concentration(0.5–1 wt.%) of nano CuO/g-C_3 N_4(g-CN)sheets within the PPSU matrix. The results of contact angle, atomic force microscopy,energy-dispersive X-ray spectroscopy reveal that surface structure and physico-chemical properties of nanohybrid membrane plays lead role in solute interaction and rejection compared to bare membrane, M0. Furthermore, the interfacial affinity of membrane was explored in detail via surface free energy, spreading coefficient, wetting tension and reversible work of adhesion analysis. Nanohybrid UF membrane, with 0.5% of the filler(M1)displayed remarkable permeation flux of 202, 131 L/m2/hr for pure water and protein solution, respectively while maintaining a high protein rejection(96%). Moreover, the exceptional dispersion of the nanosheets in the polymer matrix enhanced FRR(79%) and decreased the overall resistance of M1 compared to the pristine membrane(M0). Overall results suggest that the incorporation of nano sheets is a facile modification technique which improves the comprehensive membrane performance and holds a great potential to be further explored for water treatment.

关键词： Ultrafiltration membrane Copper oxide Graphitic carbon nitride Interfacial affinity Antifouling Protein removal