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An extract from the earthworm Eisenia fetida non-specifically inhibits the activity of influenza and adenoviruses

Journal of Traditional Chinese Medicine 2012年第4期32卷 657-663页

作者： Zhizhen Liu Jianhua Wang Jianlin Zhang Baofeng Yu Bo Niu Biochemistry and Molecular Biology Shanxi Medical University Department of Biology technology Capital Institute of Pediatrics

OBJECTIVE： To test the in vitro antiviral activity of a crude tissue extract （CTE/from the earthworm Eisenia fetida, determine any effective components in the CTE, andelucidate possiblemechanismsofaction. METHODS： A CTE was made by homogenizing earthworms, followed by treatment with ammoni- um sulfate, then thermal denaturation. Inhibition of virus-induced cytopathic effect （CPE） was used to assess antiviral activity. Chromatographic analy- sis was used to identify effective components in the CTE. RESULTS： The CTE inhibited viral CPE at non-cyto- toxic concentrations. Chromatography indicated that antiviral components corresponded to three active peaks indicative of proteases, nucleases and lysozymes. For adenoviruses, reduction in viral ac- tivity occurred for 100 lag/mL CTE. The reduction in adenoviral activity for four fractions was 100%, 91.8%, 86.9%, and 94.7%. For influenza viruses, re- duction in viral activity of 100%, 86.6%, 69.1% and 88.3% was observed for 37 pg/mL CTE. In addition, three active fractions mixture had stronger antiviral activity （98.7% and 96.7%） than three fractions *** electrophoresis results indicated that nu- cleases from E. fetida could degrade the genome of influenza viruses and adenoviruses. CONCLUSION： The earthworm CTE displayed non-specific antiviral properties, possibly mediated by a combination of proteases, nucleases and lyso- zymes. Nucleases likely participate in the antiviral process, and degrade the genome of the virus thereby preventing further replication.

关键词： Oligochaeta Complex mixtures Influ-enza a virus, HI NI subtype adenoviruses

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Ephrin A2 receptor targeting does not increase adenoviral pancreatic cancer transduction in vivo

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World Journal of Gastroenterology 2009年第22期15卷 2754-2762页

作者： Michael A van Geer Conny T Bakker Naoya Koizumi Hiroyuki Mizuguchi John G Wesseling Ronald PJ Oude Elferink Piter J Bosma Liver Center of the Academic Medical Center of the University of Amsterdam Laboratory of Gene Transfer and Regulation National Institute of Biomedical Innovation

AIM:To generate an adenoviral vector specifically targeting the EphA2 receptor(EphA2R) highly expressed on pancreatic cancer cells in ***:YSA,a small peptide ligand that binds the EphA2R with high affinity,was inserted into the HI loop of the adenovirus serotype 5 fiber *** further increase the specificity of this vector,binding sites for native adenoviral receptors,the coxsackie and adenovirus receptor(CAR) and integrin,were ablated from the viral *** ablated retargeted adenoviral vector was produced on 293T *** c targeting of this novel adenoviral vector to pancreatic cancer was investigated on established human pancreatic cancer cell *** demonstrating specifi c in vitro targeting,in vivo targeting to subcutaneous growing human pancreatic cancer was tested by intravenous and intraperitoneal administration of the ablated adenoviral ***:Ablation of native cellular binding sites reduced adenoviral transduction at least *** of the YSA peptide in the HI loop restored adenoviral transduction of EphA2R-expressing cells but not of cells lacking this ***-mediated transduction was inhibited by addition of synthetic YSA *** transduction specificity of the ablated retargeted vector towards human pancreatic cancer cells was enhanced almost 10-fold in *** a subsequent in vivo study in a nude(nu/nu) mouse model however,no increased adenoviral targeting to subcutaneously growing human pancreas cancer nodules was seen upon injection into the tail vein,nor upon injection into the ***:Targeting the EphA2 receptor increases specificity of adenoviral transduction of human pancreatic cancer cells in vitro but fails to enhance pancreatic cancer transduction in vivo.

关键词： Pancreatic cancer adenoviruses Ephrin A receptor Targeting Genetic transduction

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Molecular Characterization of Human Respiratory Adenovirus Infection in Children from November 2016 to October 2017 in Xining City, China

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Biomedical and Environmental Sciences 2019年第1期32卷 38-41页

作者： YU Juan LI Hong LU Nan Nan LEI You Ju JIANG Shuang Ying ZHAO Sheng Cang RAO Hua Xiang Center of Hygiene Inspection Qinghai Center for Disease Control and Prevention Institute for Communicable Disease Control and Prevention Qinghai Center for Disease Control and Prevention

Human adenoviruses (HAdVs) belong to the genus Mastadenovirus, family Adenoviridae, and can cause respiratory tract and gastrointestinal tract infections, as well as ocular infections in humans[1]. Till date, a total of 84 unique genotypes of AdVs have been identified and classified as human Mastadenovirus species A to G, and specific types are often associated with certain clinical symptoms, epidemiological settings, and demographic risk groups[2]. Among these species, members of the species HAdV-B (HAdV-3, HAdV-7, HAdV-11, HAdV-16, HAdV-21, HAdV-34, HAdV-35, HAdV-50, etc), HAdV-C (HAdV-1, HAdV-2, HAdV-5, and HAdV-6), and HAdV-E (HAdV-4) have been generally associated with respiratory infections[3]. HAdV infections are mild and self-limited in healthy individuals, whereas they can result in high mortality rates in immunocompetent and immunocompromized patients[4].

关键词： Human adenoviruses (HAdVs) mammivirus Eye infection

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GROWTH INHIBITION OF HUMAN LARYNGEAL CANCER CELL WITH THE ADENOVIRUS-MEDIATED p53 GENE

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Chinese Journal of Cancer Research 1999年第3期11卷 157-160页

作者：王琪韩德民王文革吴祖泽张伟 Department of Otolaryngology-Head and Neck Surgery Institute of Otolaryngology Beijing Tongren Hospital Capital University of Medical Sciences Beijing China

Objective: In most laryngeal cancers, the function of p53 gene is down regulated. To explore the potential use of p53 in gene therapy of laryngeal cancer, by introducing wild-type p53 into laryngeal cancer cell line via a recombinant adenoviral vector, Ad5CMV-p53 and analyzing its effects on cell and tumor growth. Methods: A human laryngeal cancer, cell line Hep-2 was used. Recombinant cytomegalovirus-promoted adenoviruses containing human wild-type p53 cDNA was transiently introduced into Hep-2 line. The growth suppression of the Hep-2 cells and established s.c. squamous, carcinoma model was examined. The p53 protein expression was detected using immunohistochemical analysis. Results: The transduction efficiencies of Hep-2 cell line were 100% at a multiplicity of 100 or greater. The p53 protein expression peaked on day 2 after infection and lasted far 5 days. In vitro growth assays revealed cell death following Ad5CMV-p53 infected. In vivo studies, Ad5CMV-p53 inhibited the tumorigenicity of Hep-2 cell, and in nude mice with established s.c. squamous, carcinoma, nodules showed that tumor volumes were significantly reduced in mice that received peritumoral infiltration of Ad5CMV-p53. Conclusion: Adenovirus-mediated antitumor therapy carrying the p53 gene is an efficient method to inhibit laryngeal cancer growth. Transfection of laryngeal cancer cells with the wild-type p53 gene via Ad5CMV-p53 is a potential novel approach to the therapy of laryngeal cancer.

关键词： Gene therapy Laryngeal cancer p53 gene adenoviruses

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