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Melatonin reduces acute lung injury in endotoxemic rats

Chinese Medical Journal 2009年第12期122卷 1388-1393页

作者： SHANG You XU San-peng WU Yan JIANG Yuan-xu WU Zhou-yang YUAN Shi-ying YAO Shang-long Department of A-naesthesiology and Intensive Care Medicine Union nospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430022 China Department of Neurology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430022 China Department of Pathology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Hubei 430030 China

Background Treatment with melatonin significantly reduces lung injury induced by bleomycin, paraquat and ischemia reperfusion. In the present study, we investigated the possible protective roles of melatonin in pulmonary inflammation and lung injury during acute endotoxemia. Methods Thirty-two male Sprague-Dawley rats were randomly assigned to four groups： vehicle ＋ saline group, melatonin ＋ saline group, vehicle ＋ lipopolysaccharide group, melatonin ＋ lipopolysaccharide group. The rats were treated with melatonin （10 mg/kg, intraperitoneal injection （i.p.）） or vehicle （1% ethanol saline）, 30 minutes prior to lipopolysaccharide administration （6 mg/kg, intravenous injection）. Four hours after lipopolysaccharide injection, samples of pulmonary tissue were collected. Blood gas analysis was carried out. Optical microscopy was performed to examine pathological changes in lungs and lung injury score was assessed. Wet/dry ratios （W/D）, myeloperoxidase activity, malondialdehyde concentrations and tumor necrosis factor-alpha （TNF-α） and interleukin-10 （IL-10） levels in lungs were measured. The pulmonary expression of nuclear factor-kappa B （NF-κB） p65 was evaluated by Western blotting. Results PaO2 in the vehicle ＋ lipopolysaccharide group decreased compared with that in the vehicle ＋ saline group. This decrease was significantly reduced in the melatonin ＋ lipopolysaccharide group. The lung tissues from the saline ＋ lipopolysaccharide group were significantly damaged, which were less pronounced in the melatonin ＋ lipopolysaccharide group. The W/D ratio increased significantly in the vehicle ＋ lipopolysaccharide group （6.1±0.18） as compared with that in the vehicle ＋ saline group （3.61±0.3）（P 〈0.01）, which was significantly reduced in the melatonin ＋ lipopolysaccharide group （4.8±0.25）（P 〈0.01）. Myeloperoxidase activity and malondialdehyde levels increased significantly in the vehicle ＋ lipopolysaccharide group compared with that in the vehicle ＋ saline group, which was re

关键词： endotoxemia acute lung injuly melationin

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