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N-Methylberbamine exert its antiarrythmic effect by extending the action potential through inhibiting both calcium and potassium currents

中国神经科学学会第十届全国学术会议

作者： Chen ZHOU Wei-Wei ZHANG Chao-Fei BAO Su-E HAN Li-Yao ZHANG Zhen CHAI State Key Laboratory of Biomembrane and Membrane Biotechnology College of Life SciencesPeking University College of Life Sciences Hebei University

Interleukin-1β(IL-1β) is a key proinflammatory cytokine in many injuries and diseases of central nervous system(CNS).Increasing evidences had showed the effects of IL-1β on ion channels of distinct cell types,including the majority of voltage-gated channels in excitable *** large-conductance Ca-activated K(BK) channel is widely distributed in various tissues and involved in many physiological *** chronic effects of IL-1β on BK channel and corresponding properties of action potential(AP) in cortical neurons are not clear *** we investigated the characteristics of BK channel in whole-cell and unitary channel levels and analyzed the properties of AP on cultured cortical neurons with or without IL-1β *** data determined that IL-1β don’t change the unitary BK channel characteristics and explained that the mechanism of inhibition on whole-cell BK current is resulted from reduction of Ca channels by IL-1β.The current-clamp experiments also confirmed that IL-1β decreased the amplitude of fast afterhyperpolarization(fAHP) through the inhibition on BK current In contrast,the refractory period was longer in IL-1βtreated *** data showed the chronic influences of IL-1β on BK channel and AP and suggested the implication of the effects on neuronal electrophysiological characteristics in biological functions of IL-1β.

关键词： IL-1β BK channels action potential

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N-Methylberbamine exert its antiarrythmic effect by extendin...

The Effect of Lactate on the Contractile Force of Ventricular Papillary Muscle and Cellular action potential

2019年中国生理学会心血管生理学术研讨会

作者： Huiyuan Hu Shi Zhou Xiaodong Sun Yingchun Xue Xuanxuan Sun Ming Lei Jinming Li Xiaorong Zeng Living Hao Department of Pharmaceutical Toxicology School of PharmacyChina Medical University Key Laboratory of Medical Electrophysiology of Ministry of Education Collaborative Innovation Center for Prevention and Treatment of Cardiovascular DiseaseInstitute of ardiovascular ResearchSouthwest Medical University

Objective N-methylberbamine(N-MB) is a berberine derivative,whose structure is similar to *** has been reported that berbamine hydrochloride has remarkable antiarrhythmic and ischemia protective ***,the cardiovascular protective effects of N-MB are still *** this study,the binding of N-MB to Cav1.2 calcium channel and Kv11.1 potassium channel was evaluated and the effects of N-MB on action potential and ionic currents in ventricular myocytes of rabbits and Chinese hamster ovary(CHO) cells stably transfected with hERG(human Ether-a-go-go Related Gene) were *** Molecular docking was performed to evaluate the binding of N-MB to Cav1.2 calcium channel and Kv11.1 potassium *** whole-cell patch clamp was utilized to elucidate the effects of N-MB on action potential and ionic currents in ventricular myocytes of rabbits and *** The results showed that N-MB was predicted to bind to the domain IV of Cav1.2 channel and Kv11.1 channel with higher binding affinity than their corresponding inhibitors,*** a5 min perfusion with N-MB,the durations of action potentials(APD,APD and APD) were dramatically extended from 170±45 ms,278±48 ms and 306±43 ms to395±64 ms,606±50 ms and 638±31 ms,respectively,while the amplitude of action potential(APA) was slightly reduced from 125±1.2 mV of control to 111±2.1 mV.N-MB also decreased the peak amplitude of L-type calcium current,I,without altering the reversal potential(about+60 mV).Meanwhile,neither V(-18.5±0.11 mV in control;-19.5±0.43 mV in 1 μM N-MB) nor K value(5.08±0.10 mV in control;4.80±0.43 mV in 1 μM N-MB) was ***,1 N-MB can dramatically reduce the amplitude of the outward tail currents,I to Zero level at different membrane potentials from-20 mV to+50 *** this inhibitory effect of 1μM N-MB on I was reversible after 5-10 min washing with normal Tyrode *** results which studied the effect of 1 μM N-MB on hERG channel current,IhERG

关键词： N-methylberbamine arrhythmia action potential calcium current potassium current

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中国组织工程研究 2001年第7期5卷 155-156页

作者：刘昭强朱寄天史清钊刘英杰 College of Physical Education Jimei University，Xiamen 361021China Chengdu Physical Education College Beijing Teachers'College of Physical Education

Objective To study the effect of lactate of different concentration on the action potential of ventricular papillary muscle cell (n=4) and contractile force (n=10).Method Using intracellular microelectrode technique and microtension depicting *** The result showed the effect of lactated solution of a certain comcentration became obvious. After perfused with lactate solution of 4mmol/L,the vilocity maximum decreased significantly (p<0.005) showed the 4mmol/L lactate solution decreased the rate of depolarization: decreased the efficency of Na＋ channel; lactate solution decreased the contractile force of cardiac *** The possible mechanism was:Ca＋ influx and Na＋－ H＋ exchange were inhibited, resulted in the elevation of intracellular H＋ concentratioon;through Na＋－ Ca2＋ exchange ,Ca2＋ efflux decreased resulted in the intracellular Ca2＋ decreased resulted in the intracellular Ca2＋ overload.

关键词： intracellular microelectrode in vitro lactic acid contractile force action potential papillary muscle

Axonal Conduction Velocity: A Computer Study

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Journal of Applied Mathematics and Physics 2024年第1期12卷 60-71页

作者： Arthur D. Snider Aman Chawla Salvatore D. Morgera Department of Electrical Engineering University of South Florida Tampa USA Department of Computer Science and Engineering Indian Institute of Technology New Delhi India

This paper derives rigorous statements concerning the propagation velocity of action potentials in axons. The authors use the Green’s function approach to approximate the action potential and find a relation between ... 详细信息

关键词： Neuron Axon action potential Conduction Velocity Internode

INTRINSIC FLUORESCENCE CHANGES IN NERVE TERMINALS,EVOKED BY action potentialS,ARE MODULATED BY KREBS CYCLE SUBSTRATES

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Journal of Innovative Optical Health Sciences 2011年第2期4卷 209-214页

作者： PAUL KOSTERIN ANA LIA OBAID BRIAN M.SALZBERG Department of Neuroscience University of Pennsylvania School of Medicine Philadelphia PA 19104USA Department of Physiology University of Pennsylvania School of Medicine Philadelphia PA 19104USA

Electrical stimulation of the mammalian neurohypophysial infundibular stalk evokes the entry of Nat and Ca^(2+) into the neurosecretory terminals during the action *** events,in turn,increase intracellular Ca^(2+) and activate NaK-and Ca-ATPases,prompting the mitochondria to increase oxidative phosphorylation which can be monitored by recording the changes in FAD and *** paper reflects our efforts to determine whether or not modulating the capacity of mitochondria to produce ATP,by changing the concentrations of two important substrates of the Krebs cycle of the nerve terminal mitochondria,pyruvate and glucose,has an effect on the intrinsicfluorescence changes triggered by action potential stimulation.

关键词： Fluorescence FAD NADH action potential metabolism

Dendritic Morphology Affects the Velocity and Amplitude of Back-propagating action potentials

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Neuroscience Bulletin 2022年第11期38卷 1330-1346页

作者： Wu Tian Luxin Peng Mengdi Zhao Louis Tao Peng Zou Yan Zhang State Key Laboratory of Membrane Biology College of Life SciencesPeking UniversityBeijing 100871China College of Chemistry and Molecular Engineering Synthetic and Functional Biomolecules CenterBeijing National Laboratory for Molecular SciencesKey Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationPeking UniversityBeijing 100871China Center for Quantitative Biology Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100871China PKU-IDG/McGovern Institute for Brain Research Peking UniversityBeijing 100871China Center for Bioinformatics National Laboratory of Protein Engineering and Plant Genetic EngineeringSchool of Life SciencesPeking UniversityBeijing 100871China Peking-Tsinghua Center for Life Sciences Peking UniversityBeijing 100871China Chinese Institute for Brain Research(CIBR) Beijing 102206China Beijing Academy of Artifcial Intelligence Beijing 100084China

The back-propagating action potential(bpAP)is crucial for neuronal signal integration and synaptic plasticity in dendritic *** properties(velocity and amplitude)can be affected by dendritic *** to limited spatial resolution,it has been difficult to explore the specific propagation process of bpAPs along dendrites and examine the influence of dendritic morphology,such as the dendrite diameter and branching pattern,using patch-clamp *** taking advantage of Optopatch,an all-optical electrophysiological method,we made detailed recordings of the real-time propagation of bpAPs in dendritic *** found that the velocity of bpAPs was not uniform in a single dendrite,and the bpAP velocity differed among distinct dendrites of the same *** velocity of a bpAP was positively correlated with the diameter of the dendrite on which it *** addition,when bpAPs passed through a dendritic branch point,their velocity decreased *** to velocity,the amplitude of bpAPs was also positively correlated with dendritic diameter,and the attenuation patterns of bpAPs differed among different *** results from neuron models with different dendritic morphology corresponded well with the experimental *** findings indicate that the dendritic diameter and branching pattern significantly influence the properties of *** diversity among the bpAPs recorded in different neurons was mainly due to differences in dendritic *** results may inspire the construction of neuronal models to predict the propagation of bpAPs in dendrites with enormous variation in morphology,to further illuminate the role of bpAPs in neuronal communication.

关键词： Dendrite action potential Backpropagation Synaptic integration

Effects of benactyzine on action potentials and contractile force of guinea pig papillary muscles

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郑州大学学报(医学版) 2000年第2期35卷 124-127页

作者： DING Zhongren 1)△ , ZHANG Yan 2) , ZHANG Guiqing 2) , KE Jun 2) , HU Xiangjie 2) 1)Department of Hematology,Peking Union Medical Hospital,Chinese Academy of Medical Sciences,Beijing 100730 2)Department of Pharmacology, Henan Medica Department of Hematology Peking Union Medical HospitalChinese Academy of Medical SciencesBeijing 100730 Department of Pharmacology Henan Medical UniversityZhengzhou 450052

Aim: To explore the effects of benactyzine (BEN) on the action potential and contractile force in guinea pig papillary muscles. Methods: Conventional microelectrode technique was used to record the fast action potentials (FAP) and slow action potentials (SAP) of guinea pig papillary muscles. Results: Benactyzine 5, 10, 50 μmol·L -1 suppressed the maximal upstroke velocity ( v max ) of FAP and contractile force (Fc) concentration dependently while prolonged the action potential duration at 50%, 90% repolarization (APD 50 , APD 90 ) and effective refractory period (ERP) of FAP. The suppression on the v max was frequency dependent. Benactyzine 5, 10,50 μmol·L -1 lengthened the APD50, APD90 of SAP induced by isoprenaline or histamine when perfused with KCl 22 mmol·L -1 Tyrode’s solution. The v max of the SAP was not decreased by benactyzine 5, 10 μmol·L -1 but by 50 μmol·L -1 . The effects on the SAP were antagonized by elevation of the extracellular calcium from 2.0 to 5.6 mmol·L -1 . The effects of benactyzine on SAP elicited by tetrodotoxin resembled that by isoprenaline or histamine except the more pronounced suppression on v max and action potential amplitude (APA). The persistent rapid spontaneous activity and triggered tachyarrhythmia induced by ouabain were also abolished immediately by benactyzine 5 μmol·L -1 . Conclusion: Benactyzine can inhibit Na +, K +, Ca 2+ transmembrane movement and intracellular Ca 2+ mobilization in the myocardium, and this may be the electrophysiological basis of its effects against experimental arrhythmias.

关键词： benactyzine papillary muscle action potential myocardial contraction tetrodotoxin

Memristive structure of Nb/HfOx/Pd with controllable switching mechanisms to perform featured actions in neuromorphic networks

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Nano Research 2022年第9期15卷 8410-8418页

作者： Junwei Yu Fei Zeng Qin Wan Yiming Sun Leilei Qiao Tongjin Chen Huaqiang Wu Zhen Zhao Jiangli Cao Feng Pan Key Laboratory of Advanced Materials(MOE) School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China Center for Brain Inspired Computing Research(CBICR) Tsinghua UniversityBeijing 100084China Microelectronics Institute Tsinghua UniversityBeijing 100084China School of Materials Science and Engineering University of Science and Technology BeijingBeijing 100083China

All memristor neuromorphic networks have great potential and advantage in both technology and computational protocols for artificial *** is crucial to find suitable elementary units for both performing featured neuromorphic functions and fabrication in large *** a simple memristive structure,Nb/HfOx/Pd,is proposed for this *** two resistive switching mechanisms,Mott transition of NbO2 and oxygen vacancy(Vo)migration,can be controlled by modulating external bias *** bias activates reversible phase transition and restrains Vo filament formation to allow the memristor to mimic the firing action *** bias activates Vo filament formation and restrains the other to allow the memristor to mimic synaptic plasticity and learning *** system can respond adaptively to naturally generated action potentials and modified synaptic signals from the same memristive *** addition,some special features related to signal encoding and recognition are discovered when the system is settled according to chaos circuit *** study provides a novel approach for designing elementary units for neuromorphic computations.

关键词： memristive system phase transition oxygen vacancy action potential synaptic plasticity

Electrophysiological actions of cyclosporin A and tacrolimus on rat hippocampal CA1 pyramidal neurons

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Acta Pharmacologica Sinica 2007年第12期28卷 1891-1897页

作者： Yong YU~2,Xue-qin CHEN~3,Yao-yuan CUI~(2,4),Guo-yuan HU~(3,4)~2 Department of Neurosurgery,Zhongshan Hospital,Fudan University,Shanghai 200032,China ~3 State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China Department of Neurosurgery Zhongshan Hospital Fudan University Shanghai China State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai China

Aim:The aim of the present study was to investigate the electrophysiologicalactions of cyclosporin A （CsA） and tacrolimus （FK506） on neurons in the brain,and to elucidate the relevant ***:Whole-cell current-clamprecording was made in CA1 pyramidal neurons in rat hippocampal slices;whole-cell voltage-clamp recording was made in dissociated hippocampal CA1 pyrami-dal neurons of ***:CsA （100 μmol/L） and FK506 （50 μmol/L） did notsignificantly alter the passive electrical properties of hippocampal CA 1 pyramidalneurons,but slowed down the repolarizing phase of the action ***（10-100 μmol/L） selectively inhibited the delayed rectifier K+current （IK） in aconcentration-dependent *** did not affect the kinetic properties of *** dialysis of CsA （100 μmol/L） had no effect on *** inhibition of IKby CsA （100 μmol/L） persisted under the low Ca2+conditions that blocked thebasal activity of ***:CsA exerted calcineurin-independentinhibition on the IKin rat hippocampal pyramidal *** together withour previous finding with FK506,it is conceivable that the spike broadening causedby the immunosuppressant drugs is due to direct inhibition on the IK.

关键词： action potential calcineurin cyclosporin A delayed rectifier K~+ channel hippocampus tacrolimus