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A pilot survey on antimicrobial activity and diversity of soil-derived actinobacteria from different depths in Gudzhirganskoe saline lake in Eastern Siberia

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Journal of Oceanology and Limnology 2023年第4期41卷 1307-1320页

作者： Qin YANG Puyu GUO Elena Y.ABIDUEVA Elena V.LAVRENTYEVA Irina M.LISEVICH Ilya A.OSTERMAN Petr V.SERGIEV Shaowei LIU Chunmei XUE Chenghang SUN Department of Microbial Chemistry Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China Beijing Key Laboratory of Antimicrobial Agents Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China College of Life Sciences Jiamusi UniversityJiamusi 154000China Institute of General and Experimental Biology Siberian BranchRussian Academy of SciencesUlan-Ude 670047Russia Center of Life Sciences Skolkovo Institute of Science and TechnologyMoscow 101135Russia Department of Chemistry A.N.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscow 101135Russia

To explore pharmaceutical actinobacteria from salty environments in Siberia,8 soil samples were collected from different depths of Gudzhirganskoe saline lake,the Republic of Buryatia,***,635 actinobacterial strains affiliated with 21 genera in 12 families of 7 orders were obtained by culture-dependent *** predominant genus was Streptomyces(74.5%),followed by Microbacterium(4.9%),Agromyces(3.5%),Nocardiopsis(3.0%),and Kitasatospora(2.5%).Seven strains showed relatively low 16 S rRNA similarities(actinobacterial diversity of soil samples collected in the highly alkaline and saline lake Gudzhirganskoe by culture-dependent approach and their capacity to produce antibiotics for the first time,which revealed that it deserves to make more efforts to discover new actinobacterial species and potential new antibiotics from the saline lake in Eastern Siberia.

关键词： Gudzhirganskoe saline lake actinobacteria diversity antimicrobial activities phenazine antibiotic

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Exploration of mangrove‑associated actinobacteria from South Andaman Islands,India

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Systems Microbiology and Biomanufacturing 2023年第4期3卷 702-718页

作者： Balakrishnan Meena Lawrance Anburajan Munir Ahamed Johnthini Nambali Valsalan Vinithkumar Gopal Dharani Atal Centre for Ocean Science and Technology for Islands National Institute of Ocean TechnologyMinistry of Earth SciencesGovernment of IndiaDollygunjPort Blair 744103Andaman and Nicobar IslandsIndia Marine Biotechnology Division Ocean Science and Technology for Islands GroupNational Institute of Ocean TechnologyMinistry of Earth SciencesGovernment of IndiaChennai 600100Tamil NaduIndia Department of Marine Science Bharathidasan UniversityTiruchirappalli 620024Tamil NaduIndia

In this study,a total of 1125 actinobacteria were isolated from the selected mangrove species:Avicennia marina,Rhizopora mucronata and Ceriops tagal from three study stations viz.,Minnie Bay,Carbyn’s Cove and *** these three stations,the highest number of actinobacteria was recorded in Carbyn’s Cove(64.97%),followed by(25.51%)at Burmanallah and the minimum of(9.51%)was recorded in Minnie *** number of actinobacteria was recorded from Ceriops tagal(40.44%)than the other selected mangrove species Avicennia marina(34.13%)and Rhizopora mucronata(25.42%).Among the 1,125 mangrove-associated actinobacteria,103 morphologically different isolates from the Minnie Bay station was selected for the further characterization *** antibacterial assay,30.11%of the isolates revealed inhibitory activ-ity against all tested clinical pathogens and 65%isolates displayed inhibitory activity against minimum of 04 tested clinical *** survival studies of the actinobacterial isolates also accomplished to withstand in varied NaCl and pH *** 103 isolates,all were found to synthesize gelatinase enzyme,73 isolates demonstrated amylolytic activity,38 isolates exhibited proteolytic and 63 isolates displayed urease ***,56 isolates exhibited excellent DNase activity and 71 isolates revealed positive for l-asparaginase *** our recognition,11 isolates exhibited constructive results in the production of 06 extracellular enzymes of industrial *** 103 isolates,48 isolates were confirmed by molecular level *** on the phylogenetic analysis,the isolates were categorized under the genera:Strep-tomyces,Nocardiopsis,Salinispora and Actinomadura.

关键词： Mangrove actinobacteria Avicennia marina Rhizopora mucronata Ceriops tagal

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Endophytic actinobacteria of medicinal plant Aloe vera: Isolation, antimicrobial, antioxidant, cytotoxicity assays and taxonomic study

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Asian Pacific Journal of Tropical Biomedicine 2018年第10期8卷 513-518页

作者： Ahmed Nafis Ayoub Kasrati Asma Azmani Yedir Ouhdouch Lahcen Hassani Laboratory of Biology and Biotechnology of Microorganisms Faculty of Sciences SemlaliaCadi Ayyad University Department of Biology Faculty of Sciences SemlaliaCadi Ayyad University

Objective: To explore the new sources of novel bioactive compounds having pharmaceutical and agricultural interest and to search the endophytic actinobacteria from medicinal plants. Methods: NAF-1 an endophyte actinobacteria was isolated from leaves of medicinal plant Aloe vera collected in Marrakesh, Morocco using Bennett agar as selective medium. NAF-1 was tested for its antimicrobial activity against five pathogenic bacteria such as Staphylococcus aureus PIC 53156, Micrococcus luteus ATCC381, Bacillus subtilis ATCC 14579, Pseudomonas aeruginosa DSM 50090 and Escherichia coli ATCC 8739 and four human clinic fungi belonging to the Candida, Aspergillus and Microsporum genera. Several antioxidant activities were studied such as DPPH free radical scavenging, β-carotene and linoleic acid and reducing power assays. The total of phenol and flavonoid was also calculated. Using Artemia salina shrimp assay, the cytotoxicity of NAF-1 crude extract was determined. Results: The results revealed that the actinobacteria showed a high activity(≥20 mm) against only Gram positive bacteria but it had a moderate activity(between 13 and 15 mm) against Human clinic fungi. The isolate also exhibited a LD50 of 14.20 μg/mL in the cytotoxicity assay. The result showed that the crude extract presented an interesting free radical-scavenging activity with IC50 value of(5.58 ± 0.26) μg/mL and a high value of phenolic and flavonoid compounds with(15.41 ± 0.18) μg GAE/mg extract and(11.41± 0.06) μg QE/mg extract respectively. Moreover, the taxonomic position of our endophyte actinobacteria using the morphological and physiological criteria and using 16 S r RNA gene sequence(polyphasic approach) showed that the NAF-1 isolate was similar to Streptomyces hydrogenans which was never described as an endophyte actinobacteria. Conclusions: This isolated strain appears promising resources of bioactive agents and can be exploited to produce therapeutic agents active against pathogenic disease.

关键词： actinobacteria Medicinal plant Aloe vera Antimicrobial activity Cytotoxicity Antioxidant assays Molecular identification

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Isolation and characterization of tyrosinase produced by marine actinobacteria and its application in the removal of phenol from aqueous environment

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Frontiers in Biology 2014年第4期9卷 306-316页

作者： Suki ROY Ishita DAS Minki MUNJAL Loganathan KARTHIK Gaurav KUMAR Sathish KUMAR Kokati Venkata Bhaskara RAO Molecular and Microbiology Research Laboratory School of Bio Sciences and Technology VIT University Vellore-632014 Tamil Nadu India Department of Biotechnology Shri JJT University Jhunjhunu Raiasthan India

The present study was focused on screening and characterization of tyrosinase enzyme produced by marine actinobacteria and its application in phenolic compounds removal from aqueous solution. A total of 20 strains were isolated from marine sediment sample and screened for tyrosinase production by using skimmed milk agar medium. Among 20 isolates, two isolates LK-4 and LK-20 showed zone of hydrolysis and these were taken for secondary screening by using tyrosiue agar medium. Based on the result of secondary screening LK-4 was selected for further analysis, such as tyrosinase assay, protein content and specific activity of the enzyme. The tyrosinase enzyme was produced in a SS medium and was partially purified by ammonium sulfate precipitation, dialysis and SDS PAGE. The isolate （LK-4） was identified as Streptomyces espinosus using 16S rRNA gene sequencing and named as ＂Streptomyces espinosus strain LK4 （KF806735）＂. The tyrosinase enzyme was immobilized in sodium alginate which was applied to remove phenolic compounds from water. The enzyme efficiently removed the phenolic compounds from aqueous solution within few hours which indicated that tyrosinasc enzyme produced by Streptomyces espinosus strain LK-4 can be potently used for the removal of phenol and phenolic compounds from wastewater in industries.

关键词： actinobacteria phenolic compounds immobilization tyrosinase

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actinobacteria分离株的多相分类学研究

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中国抗生素杂志 2005年第11期30卷 641-646,652页

作者：余利岩刘红宇黄明玉陈睦朱海燕张月琴中国医学科学院中国协和医科大学医药生物技术研究所 100050

A ctinobacteria class nov.一般包括具有超过50%G+C的DNA碱基组成的微生物,其中一些菌种在生物技术和医药方面具有重要的意义。为了分离能够产生有用化合物的微生物菌种,我们将分离对象从已进行过深入研究的S trep tomy ces及其以外的... 详细信息

A ctinobacteria class nov.一般包括具有超过50%G+C的DNA碱基组成的微生物,其中一些菌种在生物技术和医药方面具有重要的意义。为了分离能够产生有用化合物的微生物菌种,我们将分离对象从已进行过深入研究的S trep tomy ces及其以外的放线菌扩大到所有的A ctinobacteria,其中包括那些具有普通细菌形态的高G+C含量革兰阳性细菌。为了对A ctinobacteria这一较新的研究领域有详细的了解,我们又对两株有弱生物活性的IM B 02B-165和IM B 02B-172进行了多相分类学研究。化学分类和系统分类的结果表明IM B 02B-165、IM B 02B-172菌株均属于A rthrobacter属中A rthrobacter g lobif orm is/A rthrobacter citreus组。

关键词： actinobacteria 化学分类系统分类多相分类

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In-vitro antimicrobial activity of marine actinobacteria against multidrug resistance Staphylococcus aureus

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Asian Pacific Journal of Tropical Biomedicine 2012年第10期2卷 787-792页

作者： Sathish Kumar SR Kokati Venkata Bhaskara Rao Molecular and Microbiology Research Laboratory Environmental Biotechnology DivisionSchool of Bio Sciences and TechnologyVIT University

Objective:To investigate the antibacterial aclivily of marine actinobacteria against multidrug resistance Staphylococcus aureus(MDRSA).Methods:Fifty one actinobacterial strains were isolated from salt pans soil,costal area in Kothapattanam,Ongole,Andhra *** screening was done using cross-streak method against *** bioaclive compounds are extracted from efficient actinobacteria using solvent *** antimicrobial activity of crude and solvent extracts was perfomied using Kirby-Bauer *** for ethyl acetate extract was determined by modified agar well diffusion *** potent actinobacteria are identified using Nonomura key,Shirling and Gottlieb 1966 with Bergey's manual of determinative ***:Among the fifty one isolates screened for antibacterial activity,SRB25were found efficient against *** ethyl acetate extracts showed high inhibition against test *** test was performed with the ethyl acetate extract against MDRSA and found to be 1 000μg/*** isolaled actinobacteria are identified as Streptomyces sp with the help of Nonomura ***:The current investigation reveals that the marine actinobacteria from salt pan environment can be able to produce new drug molecules against drug resistant microorganisms.

关键词： Marine actinobacteria Salt pan Multi drug resistance Staphylococcus aureus Cross streak method Kirby-Bauer method MIC Nonomura key

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actinobacteria的分离与鉴别

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微生物学通报 2001年第5期28卷 36-41页

作者：余利岩姚天爵中国协和医科大学中国医学科学院医药生物技术研究所北京100050

actinobacteriaclassisnov .一般包括具有超过 5 0 %G +C的DNA碱基组成的微生物。实验中在分土培养基中 ,添加了Nalidixicacid及Aztreonam和Benlate ,从 4份土壤样品中 ,共分离得到 64株细菌 ,革兰氏阳性细菌共计 5 6株 ,占所分离菌株的... 详细信息

actinobacteriaclassisnov .一般包括具有超过 5 0 %G +C的DNA碱基组成的微生物。实验中在分土培养基中 ,添加了Nalidixicacid及Aztreonam和Benlate ,从 4份土壤样品中 ,共分离得到 64株细菌 ,革兰氏阳性细菌共计 5 6株 ,占所分离菌株的 87 5 %。任意挑选其中的革兰氏阳性细菌 ,选用以高G +C含量革兰氏阳性细菌为靶点的PHGC探针及PNHGC对照探针 ,并联合使用我们自行设计的适用于Actonobacteria的PA 1和PA 2探针进行初筛菌株的鉴别。综合 4种探针的FISH的结果 ,我们可以判定在 31株分离株中 ,有 2 2株Acti nobacteria,6株低G +C含量革兰氏阳性细菌 ,其它 3株则不易判定。DNAG +C含量的测定结果表明 ,所建立的FISH方法可作为鉴别actinobacteria的一种手段 ,它具有完整。

关键词： actinobacteria 革兰氏阳性高G+C含量细菌荧光原位杂交 FISH 放线菌

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Diversity and Bioactivity of Cultivable Animal Fecal actinobacteria

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Advances in Microbiology 2013年第1期3卷 1-13页

作者： Yi Jiang Li Han Xiu Chen Min Yin Dan Zheng Yong Wang Shumei Qiu Xueshi Huang Lab of Metabolic Disease Research and Drug Development China Medical University Shenyang China Sanqi Research Institute of Wenshan Wenshan China Yunnan Institute of Microbiology Yunnan University Kunming China Yunnan Wild Animal Park Kunming China

Microbial symbionts play important roles in food digestion and absorption, immunity, pathogens resistance, and health maintaining of their hosts by coevolution. To provide new sources for discovering new leader compounds of drugs, the diversity and bioactivities of cultivable actinobacteria from animal feces have been studied. 31 species of animal fecal samples were collected fromYunnanWildAnimalPark. The purified cultures of actinobacteria were isolated from these samples by using 5 media. The 16S rRNA gene sequences of 528 selected strains were determined, the phylogenetic analysis was carried out, and anti-microbial and anti-tumor activities were determined. 35 genera (including a new genus, Enteractinococcus) of actinobacteria from the 31 species of animal feces were identified. Some strains had high anti-tumor and antimicrobial activities. More than 50 secondary metabolites were isolated and identified, a novel bioactive macrolactam polyketide glycoside, Sannastatin, was found. Nine fecal streptomycete strains were fermented respectively, blended to the microbial manure, and used to prophylaxis and treatment of soil-borne disease of notoginseng in field. The incidence rate of the disease was lower 81% than agricultural chemicals. Fecal actinobacteria, a possibility as a new source for discovering drug leader, agricultural chemicals and other industry products, will be discussed.

关键词： actinobacteria Diversity Bioactivity Animal Feces

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PCR快速鉴定actinobacteria三种模板制备方法的比较

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中国抗生素杂志 2003年第7期28卷 388-390,396页

作者：徐平李文均徐丽华姜成林教育部微生物资源开放研究重点实验室云南大学省微生物研究所

目的　本研究旨在建立准确、简便、快速的放线细菌鉴定技术 ,为普通和极端环境放线细菌资源的调查和开发利用创造条件。方法　从放线细菌固体培养基上挑取少量菌体 ,用微波炉法快速制备基因组DNA作为PCR模板 ,与液体培养法得到的菌体以... 详细信息

目的　本研究旨在建立准确、简便、快速的放线细菌鉴定技术 ,为普通和极端环境放线细菌资源的调查和开发利用创造条件。方法　从放线细菌固体培养基上挑取少量菌体 ,用微波炉法快速制备基因组DNA作为PCR模板 ,与液体培养法得到的菌体以超声波法或冻融法制备的模板进行了PCR扩增效果的比较研究。结果　PCR检测结果表明微波炉法制备的模板可进行有效的体外扩增 ,目的条带特异 ,而超声波法或冻融法并不对所有菌株有效 ,并有非特异扩增产物产生。结论　组合微波炉法快速制备放线细菌基因组DNA技术和 2 3SrRNA特异插入序列PCR扩增技术建立了准确、简便、快速的actinobacteria鉴别体系。

关键词： actinobacteria 微波炉法超声波法冻融法基因组DNA提取放线细菌鉴定技术

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两种actinobacteria鉴定方法的比较

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中国抗生素杂志 2001年第1期26卷 10-14页

作者：余利岩李秋萍姚天爵中国协和医科大学中国医学科学院医药生物技术研究所北京100050

actinobacteria classis nov.一般包括具有超过 5 0 % G+ C的 DNA碱基组成的微生物。其中的一些菌种在生物技术和医药方面具有重要的意义。为了分离能产生有用物质的新型微生物 ,我们扩大了分离对象 ,从以前的 Streptomyces和 Streptomy... 详细信息

actinobacteria classis nov.一般包括具有超过 5 0 % G+ C的 DNA碱基组成的微生物。其中的一些菌种在生物技术和医药方面具有重要的意义。为了分离能产生有用物质的新型微生物 ,我们扩大了分离对象 ,从以前的 Streptomyces和 Streptomyces以外的放线菌扩大到所有的 actinobacteria。本论文实验中的actinobacteria将特指那些具有普通细菌形态的高 G+ C含量革兰氏阳性细菌。为了能够将 actinobacteria和其它革兰氏阳性低 G+ C含量的细菌加以区分 ,我们建立了两种鉴定方法。一种是荧光原位杂交 (FISH)方法 ,它具有准确和直观的优点。另外一种是 PCR方法 ,因在 actinobacteria2 3S r RNA基因的 domain (螺旋区 5 4a)有 1个大约 10 0个碱基的稳定插入片段 ,通过体外 PCR扩增 2 3S r DNA片段 ,并用琼脂糖电泳分析 ,可以简便地鉴定 actinobacteria。

关键词： actinobacteria PCR 荧光原位杂交插入片段微生物来源抗生素

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