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Preliminary studies of constructing a tissue-engineered lamellar corneal graft by culturing mesenchymal stem cells onto decellularized corneal matrix

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International Journal of Ophthalmology(English edition) 2021年第1期14卷 10-18页

作者： Yu-Jie Cen De-Bo You Wei Wang Yun Feng Department of Ophthalmology Peking University Third HospitalBeijing 100191China Beijing Key Laboratory of Restoration of Damaged Ocular Nerve Peking University Third HospitalBeijing 100191China

AIM:To construct a competent corneal lamellar substitute in order to alleviate the shortage of human corneal ***:Rabbit mesenchymal stem cells(MSCs)were isolated from bone marrow and identified by flow cytometric,osteogenic and adipogenic *** decellularized corneal matrix(XDCM)was generated from dog *** were seeded and cultured on XDCM to construct the tissueengineered ***-transplantation biocompatibility of engineered corneal graft were tested by animal *** were divided into two groups then underwent lamellar keratoplasty(LK)with different corneal grafts:1)XDCM group(n=5):XDCM;2)XDCM-MSCs groups(n=4):tissue-engineered cornea made up with XDCM and *** ocular surface recovery procedure was observed while corneal transparency,neovascularization and epithelium defection were measured and *** vivo on focal exam was performed 3 mo ***:Rabbit MSCs were isolated and *** cytometry demonstrated isolated cells were CD90 positive and CD34,CD45 *** and adipogenic induction verified their multipotent ***-XDCM grafts were constructed and *** vivo transplantation showed the neovascularization in XDCMMSC group was much less than that in XDCM group ***-transplant 3-month confocal test showed less nerve regeneration and bigger cell-absent area in XDCM-MSC ***:This study present a novel corneal tissue-engineered graft that could reduce post-operatively neovascularization and remain transparency,meanwhile shows that co-transplantation of MSCs may help increase corneal transplantation successful rate and enlarge the source range of corneal substitute to overcome cornea donor shortage.

关键词： mesenchymal stem cells xenogenic decellularized corneal matrix acellular corneal matrix neovascularization tissue-engineered cornea

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