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Knockdown of the atypical protein kinase genes GhABC1K2-A05 and GhABC1K12-A07 make cotton more sensitive to salt and PEG stress

引用

Journal of Integrative Agriculture 2024年第10期23卷 3370-3386页

作者： Caixiang Wang Meili Li Dingguo Zhang Xueli Zhang Juanjuan Liu Junji Su State Key Laboratory of Aridland Crop Science/College of Life Science and Technology Gansu Agricultural UniversityLanzhou730070China Western Agricultural Research Center Chinese Academy of Agricultural SciencesChangji 831100China College of Biology and Geographical Sciences Yili Normal UniversityYining 835000China

Activity of bc1 complex kinase(ABC1K)is an atypical protein kinase(aPK)that plays a crucial role in plant mitochondrial and plastid stress responses,but little is known about the responses of ABC1Ks to stress in cotton(Gossypium spp.).Here,we identified 40 ABC1Ks in upland cotton(Gossypium hirsutum L.)and found that the Gh ABC1Ks were unevenly distributed across 17 *** GhABC1K family members included 35 paralogous gene pairs and were expanded by segmental *** GhABC1K promoter sequences contained diverse cis-acting regulatory elements relevant to hormone or stress *** qRT-PCR results revealed that most Gh ABC1Ks were upregulated by exposure to different *** ABC1K2-A05 and Gh ABC1K12-A07 expression levels were upregulated by at least three stress *** genes were further functionally characterized by virus-induced gene silencing(VIGS).Compared with the controls,the Gh ABC1K2-A05-and Gh ABC1K12-A07-silenced cotton lines exhibited higher malondialdehyde(MDA)contents,lower catalase(CAT),peroxidase(POD)and superoxide dismutase(SOD)activities and reduced chlorophyll and soluble sugar contents under NaCl and PEG *** addition,the expression levels of six stress marker genes(Gh DREB2A,Gh SOS1,Gh CIPK6,Gh SOS2,Gh WRKY33,and Gh RD29A)were significantly downregulated after stress in the Gh ABC1K2-A05-and Gh ABC1K12-A07-silenced *** results indicate that knockdown of Gh ABC1K2-A05 and Gh ABC1K12-A07 make cotton more sensitive to salt and PEG *** findings can provide valuable information for intensive studies of Gh ABC1Ks in the responses and resistance of cotton to abiotic stresses.

关键词： cotton ABC1K abiotic stress responses expression patterns virus-induced gene silencing(VIGS)

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