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Journal of Ocean University of China 2023年第4期22卷 1049-1058页

作者： REN Qiuying WANG Yujia SUN Sha ZHANG Lingjing SUN Lechang WENG Ling LIU Guangming CAO Minjie College of Marine Food and Biological Engineering Jimei UniversityXiamen 361021China Collaborative Innovation Center for Marine Food Deep Processing Dalian Polytechnic University Dalian 116034China

abalones reveal unique taste after processing,mainly because of their abundant free amino acids(FAAs)and *** are nutrition components that can contribute to the unique ***,which factor(s)is responsible for the accumulation of FAAs still need further *** analyze the production of FAAs,we studied the variation of FAAs during 7 days of storage at 4℃.The content of taste-active amino acids,including Asp,Glu,Ser,and Gly increased by 1.7-fold,2.0-fold,3.0-fold,and 8.4-fold,*** relative activity of cathepsin L and aminopeptidase(AP)increased significantly during the cold storage *** identify AP in abalone and its function in mediating the production of FAAs,an aminopeptidase with wide substrate specificity was then extracted and purified from abalone muscle to *** AP with a molecular mass of 100 kDa exhibited its maximum activity at 30℃,pH 7.5,and was further confirmed by *** specifically inhibited the activity of AP,and metalloproteinase inhibitors EDTA,EGTA and 1,10-phenanthroline also suppressed its activity to different *** on its highest activity to substrate Leu-MCA and its peptide sequences,the purified enzyme was identified as leucine aminopeptidase(LAP).Our present study indicated the essential role of AP for FAAs accumulation during cold storage of abalone.

关键词： abalone aminopeptidase purification characterization taste endogenous protease texture

Acute toxicity of live and decomposing green alga Ulva (Enteromorpha) prolifera to abalone Haliotis discus hannai

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Chinese Journal of Oceanology and Limnology 2011年第3期29卷 541-546页

作者：王超于仁成周名江 Key Laboratory of Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences Graduate School of Tsinghua University

From 2007 to 2009, large-scale blooms of green algae (the so-called "green tides") occurred every summer in the Yellow Sea, China. In June 2008, huge amounts of floating green algae accumulated along the coast of Qingdao and led to mass mortality of cultured abalone and sea cucumber. However, the mechanism for the mass mortality of cultured animals remains undetermined. This study examined the toxic effects of Ulva (Enteromorpha) prolifera, the causative species of green tides in the Yellow Sea during the last three years. The acute toxicity of fresh culture medium and decomposing algal effluent of U. prolifera to the cultured abalone Haliotis discus hannai were tested. It was found that both fresh culture medium and decomposing algal effluent had toxic effects to abalone, and decomposing algal effluent was more toxic than fresh culture medium. The acute toxicity of decomposing algal effluent could be attributed to the ammonia and sulfide presented in the effluent, as well as the hypoxia caused by the decomposition process.

关键词： green tide macroalgal bloom Ulva (Enteromorpha)prolifera acute toxicity abalone Haliotis discus hannai

Effects of vitamin C deficiency or excess on growth performance, anti-oxidative response and fatty acid composition of juvenile abalone Haliotis discus hannai Ino

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Journal of Oceanology and Limnology 2020年第6期38卷 1936-1944页

作者： LI Xinxin FENG Xiuni LUO Kai MA Shuoli DENG Junming ZHANG Wenbing MAI Kangsen Key Laboratory of Mariculture(Ministry of Education) Key Laboratory of Aquaculture Nutrition and Feeds(Ministry of Agriculture)Fisheries CollegeOcean University of ChinaQingdao 266003China Jiaozhou Fisheries Technology Promotion Station Qingdao 266003China College of Animal Science and Technology Yunnan Agricultural UniversityKunming 650201China

A 240-day feeding trial was conducted to investigate the effects of dietary vitamin C on growth performance,anti-oxidative response,and fatty acid composition of juvenile abalone Haliotis discus hannai Ino(initial body weight:0.93±0.00 g).Three semi-purified experimental diets were formulated containing 0.00,94.52,and 9649.58 mg/kg of vitamin C supplied as *** results show that there was no significant difference in weight gain ratio,daily increment in shell length,and survival rate among the three *** dietary vitamin C(9649.58 mg/kg)significantly increased the activities of superoxide dismutase(SOD),glutathine peroxidase(GPX),glitathione-S-transferase(GST),glutathione reductase(GR),alkaline phosphatase(AKP),and lysozyme in viscera(Pabalone(Pabalone.

关键词： abalone ascorbic acid anti-oxidative capability fatty acid

The co-effect of microstructures and mucus on the adhesion of abalone from a mechanical perspective

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Biosurface and Biotribology 2021年第4期7卷 180-186页

作者： Jing Li Chuandong Ma Jun Liu Xiangwei Dong Jianlin Liu College of Mechanical and Electrical Engineering China University of Petroleum(East China)QingdaoChina College of Pipeline and Civil Engineering China University of Petroleum(East China)QingdaoChina

Reliable and reversible adhesion underwater is challenging due to the water molecules and weak layers of contaminants at the contact interface,which requires to deepen the un-derstanding of wet adhesion of biological ***,the co-effect of microstruc-tures and mucus of abalone foot on wet adhesion is investigated from both experimental and theoretical *** morphologies,adhesion force and coefficient of friction indicate that the mucus in adhesion zone is crucial for successful attachment of abalone based on capillary forces and viscous forces,and the mucus in non-adhesion zone with lower adhesion force and friction coefficient may behave as a lubricant for the *** theoretical calculation manifests that the microstructures may help abalone to form multiple liquid bridges with the secreted mucus,and significantly increase the wet adhesion force of *** findings will bring profound views into the underlying mechanisms of biological surface adhesion.

关键词： abalone capillary force microstructure mucus wet adhesion

Effects of Waterborne Cu and Cd on Anti-oxidative Response, Lipid Peroxidation and Heavy Metals Accumulation in abalone Haliotis discus hannai Ino

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Journal of Ocean University of China 2015年第3期14卷 511-521页

作者： LEI Yanju ZHANG Wenbing XU Wei ZHANG Yanjiao ZHOU Huihui MAI Kangsen The Key Laboratory of Aquaculture Nutrition and Feed of Ministry of Agriculture The Key Laboratory of Mariculture of Ministry of Education Fisheries College Ocean University of China

The aim of this study was to compare the effects of waterbome copper （Cu） and cadmium （Cd） on survival, anti-oxida- tive response, lipid peroxidation and metal accumulation in abalone Haliotis discus hannai. Experimental animals （initial weight： 7.49 g±0.01 g） were exposed to graded concentrations of waterborne Cu （0.02, 0.04, 0.06, 0.08 mg L-1） or Cd （0.025, 0.05, 0.25, 0.5 mgL-1） for 28 days, respectively. Activities of the anti-oxidative enzymes （catalase, CAT; superoxide dismutase, SOD; glutathione peroxidases, GPx; glutathione S-transferase, GST）, contents of the reduced glutathione （GSH） and malondiadehyde （MDA） in the hepatopancreas, and metal accumulation in hepatopancreas and muscles were analyzed after 0, 1, 3, 6, 10, 15, 21, 28 days of metal exposure, respectively. Results showed that 0.04 mg L-1, 0.06 mg L-1 and 0.08 mgL-1 Cu caused 100% death of abalone on the 21st, 10th and 6th day, respectively. However, no dead abalone was found during the 28-day waterborne Cd exposure at all experimental concentrations. Generally, activities of SOD and GST in hepatopancreas under all Cu concentrations followed a decrease trend as the exposure time prolonged. However, these activities were firstly increased and then decreased to the control level and increased again during Cd exposure. Activities of CAT in all Cu exposure treatments were higher than those in the control. These activities were firstly increased and then decreased to the control level and increased again during Cd exposure. Contents of MDA in hepatopancreas in all Cu treatments significantly increased first and then decreased to the control level. However, the MDA contents in hepatopan- creas were not significantly changed during the 28-day Cd exposure. The metals accumulation in both hepatopancreas and muscles of abalone significantly increased with the increase of waterborne metals concentration and exposure time. These results indicated that H. discus hannai has a positive anti-oxidative defense aga

关键词： abalone copper cadmium anti-oxidation peroxidation toxicity

Prostaglandin E_(2) involvement in the reproduction of small abalone,Haliotis diversicolor

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Aquaculture and Fisheries 2024年第5期9卷 804-811页

作者： Yawei Shen Lingling Li Xuan Luo Miaoqin Huang Caihuan Ke Weiwei You Weidong Li State Key Laboratory of Marine Environmental Science College of Ocean and Earth SciencesXiamen UniversityXiamen361102China Fujian Key Laboratory of Genetics and Breeding of Marine Organisms Xiamen UniversityXiamen361102China School of Life Sciences Xiamen UniversityXiamen361102China College of Ecology and Environment Hainan UniversityHaikou570228China

Prostaglandin E2(PGE2)has been reported to be involved in reproduction as one of the most abundant and widely distributed prostaglandins in *** gonad development and gamete production in mollusks can greatly assist artificial breeding,which is believed to promote the commercial benefits of various mollusk *** the present study,the contents of PGE2 in the gonads of abalone at different developmental stages(maturing stage,ripe stage,and spawning stage)and reproduction states(before and after spawning)were measured.A higher PGE2 level was found in the gonads of abalones at the ripe stage than other stages,and the highest PGE2 level was detected in the ovaries of female *** in the UV-stimulated *** the effects of PGE2 injection on the spawning efficiency were explored,and the gamete emission rates of female and male abalone were significantly increased after PGE2 *** qRT-PCR and in situ hybridization assay,higher expression levels and wide distribution of phospholipase A2(PLA2),cyclooxygenase 2(COX2),prostaglandin E synthases(PGES2),and prostaglandin E2 receptor 4(EP4)in the gonads of *** were observed after *** is speculated that PGE2 may promote the reproductive cycle in both male and female ***,although the regulation mechanism of PGE2 in female and male abalone might be *** results will be useful for the application of PGE2 in the abalone aquaculture practices to increase the reproduction efficiency in future.

关键词： Prostaglandin abalone Reproduction Gonad

Effects of Dietary Carbohydrates Sources on Lipids Compositions in abalone, Haliotis discus hannai Ino

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Journal of Ocean University of China 2009年第3期8卷 254-258页

作者： WANG Weifang MAI Kangsen ZHANG Wenbing XU Wei AI Qinghui YAO Chunfeng LI Huitao The Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 P.R. China

A study was conducted to evaluate the effects of dietary carbohydrates on triglyceride, cholesterol and fatty acid concentrations in abalone, Haliotis discus hannai Ino. Six semi-purified diets with different carbohydrates （dextrin, heat-treated wheat starch, wheat starch, corn starch, tapioca starch and potato starch, respectively）, all containing a carbohydrate level of 33.5%, were fed to abalone （initial shell length： 29.98mm±0.09mm; initial weight. 3.42g±0.02g） for 24 weeks in a recirculation system. The results indicate that serum triglyceride concentrations were significantly （P〈0.05） higher in the abalone fed with dextrin, heat-treated wheat starch and wheat starch than those fed with corn starch, and serum cholesterol concentrations were significantly （P〈0.05） higher in the abalone fed with dextrin, heat-treated wheat starch than those fed with corn starch. Fatty acid C20：4n-6 in the foot mus- cles were significantly （P〈0.05） lower in the abalone fed with dextrin than those fed with wheat starch, corn starch, tapioca starch and potato starch. Fatty acid C20：4n-6 in hepatopancreas was significantly （P〈0.05） lower in abalone fed with heat-treated wheat starch than those fed with corn starch, tapioca starch and potato starch. Fatty acid C22：6n-3 in the foot muscles were significantly （P 〈 0.05） lower in the abalone fed with dextrin and beat-treated wheat starch than those fed with wheat starch and potato starch.

关键词： abalone Haliotis discus hannai carbohydrates lipid fatty acid

Determination of Content of Amino Acid in abalone by Precolumn Derivatization and its Nutritional Value Evaluation

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Agricultural Biotechnology 2018年第3期7卷 178-183,210页

作者： Wen CHEN Xiangjun WANG Yujie WANG Yuzhi HUANG Fanmeng MENG Hui CHEN School of ocean science and technology Hainan Tropic Ocean University Sanya 572022 China Hainan Marine food engineering technology research center Sanya 572022 China School of life science and ecology Hainan Tropic Ocean University Sanya 572022 China

[Objectives] This study was conducted to optimize the determination conditions of amino acids from abalone. [Methods] The sample was treated by acid hydrolysis method and subjected to 2,4-2 nitro fluorobenzene column derivatization. The amino acid content in abalone was determined by HLPC,and the nutritional value of the amino acids was evaluated with egg protein model put forward by Institute of Nutrition and Food Hygiene,Chinese Academy of Preventive Medicine. [Results] abalone contains full amino acids. According to the FAO/WHO ideal,it is a high-quality protein source and suitable for supplement of protein source for human body. [Conclusions]The experimental method has simple operation and could achieve a good effect with wide linear range and correlation coefficient over 0. 999 8,and the obtained results are satisfactory.

关键词： abalone Precolunm derivatization HPLC Amino acid Evaluation

First steps on technological and genetic improvement of European abalone (<i>Haliotis tuberculata</i>) based on investigations in full-sib families

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Open Journal of Genetics 2013年第3期3卷 224-233页

作者： Valérie Roussel Julien Charreyron Sylvain Labarre Alain Van Wormhoudt Sylvain Huchette France Haliotis Plouguerneau France MNHN UMR 7208 BOREA Station de Biologie Marine Concarneau France

The European abalone Haliotis tuberculata is of economical importance in Europe, and recently in France where most of the consumed abalones remained wild animals fished on the coast. Recently, the creation of hatcheries allowed the production of cultured animals, and aquaculture is in progress. To optimize selective breeding programs, different studies were performed on adults and on their progenies. First, ten adult families, assumed to be bi-parental and produced in 2007 were analyzed. As these families were developed at the beginning of the hatchery production, the parentage of the individual necessitated to be tested. The progenies parentage assignment was done by using eight microsatellite DNA loci. In fine, the heritability was estimated from the analysis of variance of seven full-sib families which include from 23 to 27 progenies. Heritability estimate was the higher for length, width and weight (0.37, 0.29 and 0.40 respectively). A high correlation was also shown between these heritable traits which can be useful during animal breeding. Shell color traits were also analyzed, using image treatment procedure. Two traits were selected according to the global color of the shell: red color and hue. The distribution of these traits evidenced a polygenic genetic control of shell color and measure of heritability evidenced a high to medium value for red color (Cr) and Hue(H) (0.33 and 0.20 respectively). No correlation was shown between growth and this parameter. In a second time, juveniles families produced in 2010 were studied from parents issued of the first selection. A correlation between size and density at juvenile stage was established but a statistical analysis demonstrated, by using density as co-variable a significant effect of family on size. In these conditions, growth is highly heritable (0.74). This study is a first step toward the use of genetic markers for selection, but also a step toward the breeding improvement of European abalone.

关键词： European abalone Microsatellite Heritability Morphological Traits Shell Color Traits Juvenile

Rheological Properties and Structural Changes in Different Sections of Boiled abalone Meat

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Journal of Ocean University of Qingdao 2003年第1期2卷 44-48页

作者： GAOXin TANGZhixu ZHANGZhaohui OgawaHiroo DepartmentofFoodScienceandTechnology TokyoUniversityofFisheriesTokyo108-8477Japan FisheriesCollege OceanUniversityofChinaQingdao266003P.R.China

Changes in tissue structures, rheological properties of cross- and vertical section boiled abalone meat were studied in relation to boiling time. The adductor muscle of abalone Haliotis discus which was removed from the shell, was boiled for 1, 2, and 3 h, respectively. Then it was cut up and separated into cross- and vertical section meat. When observed by a light microscope and a scanning electron microscope, structural changes in the myofibrils were greatest in the cross section meat compared with the vertical section meat. When boiling time was increased from 1 h to 3 h, the instantaneous modulus E 0 and rupture strength of both section meat decreased gradually with increased boiling time, and no significant differences were observed between these two section meat for the same boiling time. When boiled for 1 h, the relaxation time of cross section meat was much longer than that of vertical section meat. There were no significant changes in the relaxation time of vertical section for different boiling time, but the relaxation time of cross section meat was reduced gradually with increasing boiling time. These results confirmed that the difference in rheological properties between the cross- and vertical section meat was mainly due to the denaturation level of myofibrils when heated for 1 h, as well as due to the changes in the amount of denatured proteins, and the manner in which the inner denatured protein components weve exchanged after boiling time was increased from 1 h to 3 h.

关键词： abalone meat boiling rheology