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Inhibition of matrix metalloproteinases expression in human dental pulp cells by all-trans retinoic acid

International Journal of Oral Science 2014年第3期6卷 150-153页

作者： Jin Man Kim Sang Wook Kang Su-Mi Shin Duck Su Kim Kyong-Kyu Choi Eun-Cheol Kim Sun-Young Kim Department of Conservative Dentistry School of Dentistry Kyung Hee University Department of Maxillofacial Tissue Regeneration School of Dentistry Kyung Hee University

All-trans retinoic acid（ATRA） inhibits matrix metalloproteinase（MMP）-2 and MMP-9 in synovial fibroblasts, skin fibroblasts,bronchoalveolar lavage cells and cancer cells, but activates MMP-9 in neuroblast and leukemia cells. Very little is known regarding whether ATRA can activate or inhibit MMPs in human dental pulp cells（HDPCs）. The purpose of this study was to determine the effects of ATRA on the production and secretion of MMP-2 and-9 in HDPCs. The productions and messenger RNA（mRNA） expressions of MMP-2 and-9 were accessed by gelatin zymography and real-time polymerase chain reaction（PCR）, respectively. ATRA was found to decrease MMP-2 level in a dose-dependent manner. Significant reduction in MMP-2 mRNA expression was also observed in HDPCs treated with 25 mmol？L21ATRA. However, HDPCs treated with ATRA had no effect on the pattern of MMP-9 produced or secreted in either cell extracts or conditioned medium fractions. Taken together, ATRA had an inhibitory effect on MMP-2 expression in HDPCs,which suggests that ATRA could be a candidate as a medicament which could control the inflammation of pulp tissue in vital pulp therapy and regenerative endodontics.

关键词： all-trans retinoic acid human dental pulp cell matrix metalloproteinase zymography

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Study on the Effects of FCu-IUD and FICu-IUD on Matrix metalloproteinases in Human Uterine Flushing and Endometrium

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2002年第1期22卷 9-11,16页

作者：李玮曹路敏陈早姣李万杜庆玲陈桂萍 Family Planning Research Institute Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Institute of Women and Children Health of Wuhan Steel and Iron Group Wuhan 430080

The activity of matrix metalloproteinases (MMPs) in the uterine flushing and endometrial tissue of normal adult women wearing FCu IUD (fixed Cu IUD) or FICu IUD (indomethacin releasing FCu IUD) was observed by using zymography on SDS PAGE containing gelatin. The results showed that the activity and kinds of MMPs in FCu IUD group were increased significantly as compared with themselves before being inserted FCu IUD. However, compared with the FCu IUD group, the activity of some kinds of MMPs in the FICu IUD group was decreased significantly. These data suggest that IUD can enhance the activity of MMPs in human endometrium, intermediated by prostaglandins, and MMPs may have relation to IUD induced menorrhagia and indomethacin reduces IUD induced menorrhagia by partly inhibiting MMPs synthesis.

关键词： matrix metalloproteinase IUD uterine flushing endometrium zymography

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Matrix Metalloproteinase MMP-9 Promotes K/BxN Serum Induced Arthritis in Mice

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Open Journal of Rheumatology and Autoimmune Diseases 2014年第1期4卷 22-28页

作者： Narendiran Rajasekaran Harald Illges Department of Natural Sciences Immunology and Cell Biology University of Applied Sciences Rheinbach Germany

Matrix metalloproteinases (MMPs) are matrix-degrading enzymes that are over-expressed in joints of rheumatoid arthritis (RA) patients. However, the contribution of specific MMPs for the development of arthritic joints is unknown. This study is aimed at studying the role of matrix metalloproteinase-9 (MMP-9) in mice, using the K/BxN serum-transfer model of RA. Arthritis was induced in Balb/c mice by injecting K/BxN serum. Development of arthritis was followed in these mice by measuring ankle thickness and clinical index score. MMP-9 expression in the joints of mice killed at various time points during the disease progression was determined by gelatin zymography using ankle lysates. We found that MMP-9 expression increased with the severity of arthritis. Importantly MMP-9 deficient mice injected with K/BxN serum showed a milder form of arthritis in comparison to the control C57BL/6 mice injected with K/BxN serum. We therefore conclude that MMP-9 promotes arthritis in mice.

关键词： MMP-9 Antibody Induced Arthritis K/BxN Gelatin zymography

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Effects of cytokines,growth factors and drugs on matrix metallo proteinases activities of osteoarthritic chondrocytes and synovio cytes

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Journal of Medical Colleges of PLA(China) 2001年第3期16卷 200-202页

作者：管剑龙韩星海施桂英袁国华 Department of Rheumatology and Immunology Changhai Hospital Second Military Medical University Shanghai 200433 China Department of Rheumatology General Hospital of PLA Beijing 100853 China

Objective: To evaluatetheeffectsof somecytokines,TGF-β 1 anddrugson matrixmetalloproteinases(MMPs)activitiesinculturemediumof ***:Thechondrocyteandsynoviocytemono-layersisolatedfromthecartilagesandsynovialfluidsin10kneeOA patientsweretreatedwithIL-1βTGF-β 1 ,TNF-α,di-clofenacacid,dexamethasoneor *** determinetheac-tivitiesof ***:ThechondrocytemonolayersproducedMMP-2and -9,***-9activitywasmarkedlyenhancedby IL-1βTNF-α***-1βwasthemosteffective stimulus,andhadsynergisticeffectwithTNF-αor ***-2activitywas ***,TGF-β 1 and dexamethasonecoulddepresstheactivitiesof MMP-9andMMP-2,and antagonizetheenhancingeffectof IL-1βTNF-αor *** sion:IL-1βandTNF-αmayplayimportantrolesdegradingOA cartilage,whileTGF-β 1 anddoxycy-clinemaybe protectivefactors.

关键词： chondrocytes synoviocytes matrix metalloproteinases zymography

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