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Copy number and zygosity determination of transgenic rapeseed by droplet digital PCR

Oil Crop Science 2017年第2期2卷 84-94页

作者： Yuhua Wu Jun Li Xiaying Li Jingang Liang Yunjing Li Xinhua Zeng Gang Wu Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture Oil Crops Research InstituteChinese Academy of Agricultural Sciences Wuhan China Science and Technoloty Center of Ministry of Agriculture China

Establishing an accurate and rapid method for copy number and zygosity determination can accelerate genetic engineering research process. In this study, droplet digital PCR (ddPCR), an emerging DNA absolute quantification technology, was used to identify single-copy homozygous transgenic lines from a batch of T0 transgenic rapeseed harboring 11 exogenous elements. Copy number of exogenous gene was evaluated in T0 generation based on calculated ratio between transgene and reference CruA gene, single-copy transformants were selected for selfing followed by subsequent zygosity analysis. Single-copy homozygous transgenic plants were successfully screened out in T1 generation by *** analysis with T2 seedlings verified that identification results of ddPCR were accurate and reliable. This study provides a novel rapid and accurate method for copy number and zygosity determination in transgenic rapeseed which overcomes disadvantages of traditional Southern analysis and recently developed real-time quantitative method.

关键词： droplet digital PCR (ddPCR) copy number zygosity homozygotes transgenic rapeseed

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Assessment of Dental Caries and Oral Hygiene Status Among Twins

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Journal of Forensic Science and Medicine 2018年第1期4卷 18-22页

作者： Priya Subramaniam K L Girish Babu Bharath Vardhana Department of Pedodontics and Preventive Dentistry The Oxford Dental College and HospitalBengaluru1Departmentof DentistryHassan Institute of Medical SciencesHassanKarnatakaIndia

The study of twins provides a unique opportunity to evaluate the genetic and nongenetic contributions to a variance of multitude of *** research has revealed the impact of a strong genetic component to the variance of many dental *** present study was conducted to assess the dental caries and oral hygiene status in monozygotic(MZ)and dizygotic(DZ)twins.A random sample of 129 MZ and DZ twin pairs aged 6-15 years were included in this *** zygosity of twin pairs was initially recorded by facial appearance and later determined by *** caries and oral hygiene status were recorded in these twin pairs and the data were subjected to statistical *** significant difference was observed in the mean decayed,extracted due to caries and filled teeth(deft)in primary dentition and decayed,missing and filled(DMFT)in permanent dentition scores and oral hygiene status in MZ and DZ twin pair&The similarity observed in oral health status reflects the effects of a shared environment in MZ and DZ twin pairs.

关键词： Dental caries dermatoglyphics facial appearance oral hygiene index-simplified zygosity

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COMPREHENSIVE STUDY OF TWO SETS OF TRIPLETS

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Chinese Science Bulletin 1989年第19期34卷 1643-1647页

作者：杨汉民马慰国林田赵叔鑫胡炳蔚杜耀仁李生斌李小莉 Xunyi County Hospital XunyiShanxi Research Institute of Chinese Traditional Medicine.Xi'an Xi'an Medical University The Second Subsidiary Hospital of Xi'an Medical University

Triplets are seldom and monozygotic triplets are scarcely seen in humanity. There are few reports on triplets at home and abroad and diagnosis of zygosity is rarely made. We did the analysis of clinical material on two sets of triplets. Diagnosis and analysis of zygosity were made in eleven ways about 57 items including blood type and RBC enzyme type, etc.

关键词： multiple pregnancy triplets zygosity diagnosis hereditary

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Challenges for the Characterization of Genetically Modified Animals by the qPCR Technique in the Era of Genomic Editing

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Veterinary Science Research 2021年第1期3卷 1-11页

作者： Ribrio Ivan Tavares Pereira Batista Darcio Italo Alves Teixeira Vicente Jose de Figueiredo Freitas Luciana Magalhaes Melo Joanna Maria Goncalves Souza-Fabjan Laboratorio de Fisiologia e Controle da Reproducao Faculdade de VeterinariaUniversidade Estadual do Ceara(UECE)Av.Dr.Silas Munguba 1700FortalezaCE60740-903Brazil Faculdade de Veterinaria Universidade Federal Fluminense(UFF)Rua Vital Brazil Filho64NiteroiRJ24230-340Brazil Molecular Genetics Research Unit University Center Fametro(Unifametro)Rua Conselheiro Estelita500FortalezaCE60010-260Brazil

Characterization of genetically modified organisms through determination of zygosity and transgene integration concerning both copy number and genome site is important for breeding a transgenic line and the use of these organisms in the purpose for which it was *** blot,fluorescence in situ hybridization or mating are demanding and time-consuming techniques traditionally used in the characterization of transgenic organisms and,with the exception of mating,give ambiguous *** the emergence of the real-time quantitative PCR technology,different applications have been described for the analysis of transgenic organisms by determination of several parameters to transgenic ***,the accuracy in quantitation by this method can be influenced in all steps of *** review focuses on the aspects that influence pre-analytical steps(DNA extraction and DNA quantification methods),quantification strategies and data analysis in quantification of copy number and zygosity in transgenic animals.

关键词： Absolute quantification Copy number DNA extraction Relative quantification zygosity

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