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Variations in the content of zeatin during tuber development of sweet potato and Ipomoea trifida

西南师范大学学报(自然科学版) 1999年第5期 576-580页

作者： Zhang Qitang 1,Fu Yufan 1,Xiong Fengqi 2,Yuan Lüjiang 2 1 Chongqing Sweet Potato Scientific Research Center,Southwest China Normal University,Chongqing 400715 2 Central Laboratory,Southwest China Agricultural University,Chongqing 400716

The contents of zeatin in different tuberous roots and fibrous roots of sweet potato and Ipomoea trifida at several periods of growth were determined by applying high performance liquid chromatography(HPLC).The results are as follows:After being planted for 60 days,90 days or 120 days,the contents of zeatin in 30～70 g,and 100～200 g tuberous root of sweet potato are distinctly higher than those in *** and fibrous roots of sweet potato of 0 2～0 5 cm in diameter during the same period of *** during the three periods of growth mentioned above,the content of zeatin in fibrous roots of sweet potato is indistinctly different from that of *** .A comparison of the content of zeatin during development shows that,the content of zeatin after being planted 120 days is remarkably higher than that of zeatin after being planted for 60 days and 90 days, but the difference between those being planted for 60 days and 90 days is not distinct.

关键词： sweet potato Ipomoea trifida zeatin HPLC

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Influence of Carbohydrates, Mineral Nutrients and Plant Hormones in Alternate Bearing of Black Pepper (<i>Piper nigrum</i>L.)

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American Journal of Plant Sciences 2013年第10期4卷 1960-1967页

作者： K. S. Krishnamurthy S. J. Ankegowda V. Srinivasan S. Hamza Indian Institute of Spices Research Kozhikode India.

Alternate bearing habit, a common phenomenon prevalent in some of the fruit trees is observed in black pepper variety Panniyur-1 also. In this study, an attempt was made to investigate the role of carbohydrates, mineral nutrients and plant hormones (IAA and zeatin riboside) in alternate bearing in Panniyur-1 variety. The experiment was conducted for three consecutive years at Murugarajendra estate, Madikeri, Karnataka, India (12.42。N, 75.73。E). Carbohydrate, mineral nutrient and plant hormone (IAA and zeatin riboside) statuses of the leaves as well as fruiting branches (stem) were compared between good fruiting year (on year) and low fruiting year (off year). Results revealed that concentration of mineral nutrients in leaves was more during on year compared to off year while the reverse was true on the stem. Both stem and leaf metabolite levels at harvest were higher in off year compared to on year suggesting that remobilization of nutrients to the developing berries must have been affected during off year, thus leading to accumulation of more nutrients at harvest in off year compared to on year. Leaf had higher concentration of most of mineral nutrients compared to stem. Spraying of 1% solution of complex fertilizer 19:19:19 (percent N:P:K) three times at an interval of 3 weeks from flower primordial initiation to flowering period enhanced the yield to the extent of around 30% during off year. Auxin to cytokinin ratio was 6.6 and 6.1 in on year and 6.3 and 5.7 in off year at flowering and 3 months after flowering respectively, suggesting that this ratio itself may not play a major role in flowering during on and off years, though the ratio was slightly more during on year. Results of the study indicate that efficient utilization of metabolites and nutrients in on year may render vine weak in the subsequent year coupled with poor remobilization into developing berries, which could make it an off year.

关键词： Remobilization Starch IAA zeatin Riboside Reducing Sugars Essential Oil Oleoresin

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玉米自交系掖478和TY4幼胚不定芽再生体系的优化

玉米自交系掖478和TY4幼胚不定芽再生体系的优化

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作者：王婷婷山东农业大学

学位级别：硕士

玉米幼胚是玉米组织培养中最常用的外植体,它被广泛的应用于科研工作中。优化玉米幼胚的组织培养体系,对于构建高效遗传转化体系具有重要意义。组织培养体系的优化可以从培养基的种类、添加的激素种类、添加的激素浓度等因素研究。优良... 详细信息

玉米幼胚是玉米组织培养中最常用的外植体,它被广泛的应用于科研工作中。优化玉米幼胚的组织培养体系,对于构建高效遗传转化体系具有重要意义。组织培养体系的优化可以从培养基的种类、添加的激素种类、添加的激素浓度等因素研究。优良玉米自交系掖478和TY4的组织培养都表现出愈伤组织诱导容易而绿苗分化较难的特点。在本研究中,首先对两种自交系分别在常用的三种培养基(N6培养基、改良N6培养基、MS培养基)上培养14 d后,统计II型愈伤组织诱导率。结果显示,自交系Ye478的最适培养基为N6培养基,自交系TY4的最适培养基为MS培养基。在此基础上,在愈伤组织诱导阶段分别添加不同浓度(0 mg·L、1 mg·L、2 mg·L、3mg·L)的外源激素Dicamba,统计胚性愈伤诱导率等性状,以此筛选最适激素浓度。结果显示掖478的最适Dicamba浓度为3 mg·L,TY4的最适Dicamba浓度为2 mg·L。在分化阶段对两种自交系分别添加不同浓度(0 mg·L、3 mg·L、5 mg·L、7 mg·L)的zeatin外源激素,统计绿苗分化率等性状,以此筛选最适激素浓度。结果显示zeatin浓度5 mg·L为自交系掖478分化培养的最适浓度,zeatin浓度3 mg·L为自交系TY4分化培养的最适浓度。在培养过程中发现,两种自交系在添加外源激素zeatin的分化培养基培养与对照培养基培养相比,能够提前两周生长出绿苗植株,缩短了分化出苗的时间。总之,本研究通过基本培养基比较并对培养过程的外源激素Dicamba和zeatin的最适浓度进行筛选,优化了玉米自交系掖478和TY4的芽再生体系,这一结果为进一步构建高效遗传转化体系奠定了基础。

关键词：幼胚愈伤组织诱导率绿苗分化率 Dicamba zeatin

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陆地棉野生种与栽培种间纤维发育差异的蛋白质组学研究（英文）

陆地棉野生种与栽培种间纤维发育差异的蛋白质组学研究（英文）

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中国农学会棉花分会2017年年会暨第九次会员代表大会

作者：覃元魏恒玲孙会茹郝蓬勃王寒涛宿俊吉喻树迅中国农业科学院棉花研究所/棉花生物学国家重点实验室

Upland cotton（Gossypium hirsutum L.） is one of the world’s most important fiber crops,accounting for more than 90%of all cotton *** their wild progenitors have relatively short and coarse,often tan-colored fibers,modern cotton cultivars possess longer,finer,stronger,and whiter *** this study,the wild and cultivated cottons（YU-3 and TM-1） selected show significant differences on fibers at 10 day post-anthesis（DPA）,20 DPA and mature stages at the morphological *** order to explore the effects of domestication,reveal molecular mechanisms underlying these phenotypic differences and better inform our efforts to further enhance cotton fiber quality,an iTRAQ-facilitated proteomic method was performed on developing *** were 6990 proteins identified,among them 336 were defined as differentially expressed proteins（DEPs） between fibers of wild versus domesticated *** down-or up-regulated proteins in wild cotton were involved in phenylpropanoid biosynthesis,zeatin biosynthesis,fatty acid elongation and other *** analysis between transcriptome and proteome showed positive correlations between transcripts and proteins at both 10 DPA and 20 *** in proteomics have been verified at the mRNA level by qPCR and have been validated at the physiological and biochemical levels by POD（peroxidase） activity assays and ZA（zeatin） content *** work corroborates the major pathways involved in cotton fiber development and demonstrates that POD activity and zeatin content have a great potential related to fiber elongation and thickening.

关键词： Gossypium hirsutum fiber development domestication iTRAQ phenylpropanoid zeatin

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