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Characterization of the imprinting and expression patterns of zag2 in maize endosperm and embryo

The Crop Journal 2015年第1期3卷 74-79页

作者： Chaoxian Liu Jiuguang Wang Xiupeng Mei Xiaojing Deng Tingting Yu Xiaoli Liu Guoqiang Wang Zhizhai Liu Yilin Cai Maize Research Institute Southwest University

zag2 has been identified as a maternally expressed imprinted gene in maize endosperm.Our study revealed that paternally inherited zag2 alleles were imprinted in maize endosperm and embryo at 14 days after pollination(DAP), and consistently imprinted in endosperm at 10, 12, 16, 18, 20, 22, 24, 26, and 28 DAP in reciprocal crosses between B73 and Mo17. zag2 alleles were also imprinted in reciprocal crosses between Zheng 58 and Chang7-2 and between Huang C and 178. zag2 alleles exhibited differential imprinting in hybrids of 178 × Huang C and B73 × Mo17, while in other hybrids zag2 alleles exhibited binary imprinting. The tissue-specific expression pattern of zag2 showed that zag2 was expressed at a high level in immature ears, suggesting that zag2 plays important roles in not only kernel but ear development.

关键词： Gene imprinting zag2 Expression pattern Endosperm Embryo

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Functional Analysis of the zag2 Promoter from Maize in Transgenic Tobaccos

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Journal of Integrative Agriculture 2012年第8期11卷 1266-1273页

作者： LU Min-hui WANG Guo-ying MENG Zheng WANG Jian-hua Plant Genetic and Breeding China Agricultural University Beijing 100193 P.R.China Institute of Crop Sciecnes Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Center for Signal Transduction and Metabolomics Institute of Botany Chinese Academy of Sciences Beijing 100093 P.R.China

The function of the 3 040 bp sequence at the upstream translation starting site （ATG） of the zag2 gene, isolated from the maize genome, was studied. The sequence analysis showed that the sequence contained a typical class C MADS-box gene regulatory element. The 5′ UTR region of the gene contains a 1 299-bp intron that might have important regulatory functions. To study the sequence function, deletion derivatives of promoter-reporter （uidA） gene fusions were generated and transformed into tobaccos. The GUS staining and fluorescence quantification results showed that the GUS activity was detected only in the third and fourth whorl floral organs of the transgenic tobaccos under driving the promoter including the first intron, while detected in all the organs and was stronger under driving the promoter without the first intron. However, the GUS activity was just detected in one whorl of the fourth or third floral organs under driving of the 35S promoter. These results suggested that the first intron of the zag2 gene contains functional regulatory elements, which turned out to be important for gene expression in the heterologous systems. Moreover, the GUS activity was decreased when the reporter gene driven by the promoters with 5′-deletions, respectively, from -1 606 to -951 and -951 to -426 nts, which indicates that positive regulatory elements are present in these two sequence stretches.

关键词： maize MADS-box gene zag2 intron

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