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Microsatellite Marker-based Analysis of the Genetic Diversity of yellow river carp

Agricultural Biotechnology 2017年第3期6卷 43-46,50页

作者： Youbing YANG Xiuying MA Wei WANG Youzhi PANG Wanguang CHEN College of Animal Science and Technology Henan University of Science and Technology College of Life Science Luoyang Normal University

To provide accurate base data about the genetic sourees of yellow river carps, the genetic diversity in a^ficially bred population and wild population of yellow river carps from Henan Province was analyzed with mierosatellite markers. The results showed that 16 alleles were detected at six microsateUite loci in each population. The average effective number of alleles （Ne） was 2. 350 in artificially bred population and 2. 085 in wild population. The observed heterozygosity （Ho） of artificially bred population, wild population and mixed population was 0. 614, 0. 576 and 0. 601 ; and the unbiased expected heterozygesity （ He ） was 0. 569, 0.535 and 0.559 ; and the polymorphism infonnatian content （PlC） was 0.474, 0.428 and 0.468, respectively. The PIC of the six loci ranged from 0.304 to 0. 864. The analysis of the genetic differentiation for the six microsatellitc loci in the two populations showed that the genetic differentiation coefficient （ F,, ） at only one microsatellite locus HLJ483 was greater than 0.05, and that at five rnicrosatellite loci were less than 0.05, which was consistent with the standard of non- genetic differentiation between populations （F,, = 0 -0.05）. The average F,, at the six loci was 0.02, and the gene flow value （Nm） at all loci was greater than 1 and the average of Nm was 12.202. The results indicate that there is relatively abundant genetic diversity in yellow river carps.

关键词： yellow river carp Microsatellite maker Genetic diversity Allele Genetic differentiation coefficient

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