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The Endosperm-Specific Expression of yucca genes Enhances Rice Grain Filling

Phyton-International Journal of Experimental Botany 2022年第12期91卷 2633-2648页

作者： Huijun Jiang Kaien Zhai Xiaofan Ye Tianwei Hu Jieming Jiang Xiaoqiu Dong Weihuai Pan Jianwei Pan Jianxin Shou College of Life Sciences Shaoxing UniversityShaoxing312000China College of Chemistry and Life Sciences Zhejiang Normal UniversityJinhua321004China School of Life Sciences Lanzhou UniversityLanzhou730000China School of Life Science South China Normal UniversityGuangzhou510631China

Grain filling is a crucial process that affects yield in rice(Oryza sativa L.).Auxin biosynthesis and signaling are closely related to rice yield;therefore,it is important to understand the effects of auxin biosynthesis on rice grain filling to improve crop yield.In this study,we used physiological and molecular strategies to identify the roles of auxin in rice grain filling.Exogenous application of auxin(IAA)or auxin analogues(2,4-D)to young spikelets and flag leaves improved the seed-setting rate and yield per spike.Furthermore,real-time quantitative PCR assays confirmed that nine members of the Osyucca family of auxin biosynthetic genes were upregulated during grain filling,implication that auxin biosynthesis plays a major role in grain development.The specific expression of either Arabidopsis Atyucca1 or Osyucca2 in the endosperm or leaves resulted in increased expression of OsIAA genes and auxin content of seeds,as well as increased grain filling and seed-setting rate.This result establishes that the auxin content in grains and leaves is important for grain development.Our findings further highlight the potential applications for improving rice yield by elevating targeted gene expression in specific tissues.

关键词： Auxin content grain filling IAA biosynthesis rice seed-setting rate yucca genes

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Auxin biosynthesis by the yucca6 flavin monooxygenase gene in woodland strawberry

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Journal of Integrative Plant Biology 2014年第4期56卷 350-363页

作者： Hong Liu Wei-Fa Xie Ling Zhang Victoriano Valpuesta Zheng-Wen Ye Qing-Hua Gao Ke Duan Forestry and Fruit Tree Research Institute Shanghai Key Laboratory of Protected Horticultural Technology Shanghai Academy of Agricultural Sciences (SAAS) Shanghai Key Laboratory of Agricultural Genetics and Breeding Institute of Biotechnology Department of Molecular Biology and Biochemistry Universidad de Málaga

Auxin has been regarded as the main signal molecule coordinating the growth and ripening of fruits in strawberry, the reference genomic system for Rosaceae. The mechanisms regulating auxin biosynthesis in strawberry are largely elusive. Recently, we demonstrated that two yucca genes are involved in flower and fruit development in cultivated strawberry. Here, we show that the woodland strawberry （Fragaria vesca L.） genome harbors nine loci for yucca genes and eight of them encode functional proteins. Transcription pattern in different plant organs was different for all eight FvYUCs. Functionality of the FvYUC6 gene was studied in transgenic strawberry over- expressing FvYUC6, which showed typical high-auxin pheno- types. Overexpression of FvYUC6 also delayed flowering and led to complete male sterility in F. vesta. Additionally, specific repression of FvYUC6 expression by RNA interference signifi- cantly inhibited vegetative growth and reduced plant fertility. The development of leaves, roots, flowers, and fruits was greatly affected in FvYUC6-repressed plants. Expression of a subset of auxin-responsive genes was well correlated with the changes of FvYUC6 transcript levels and free indole-3-acetic acid levels in transgenic strawberry. These observations are consistent with an important role of FvYUC6 in auxin synthesis, and support a main role of the gene product in vegetative and reproductive development in woodland strawberry.

关键词： Auxin biosynthesis Fragaria vesta plant development RNAinterference yucca genes

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