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Morphological and molecular identification of xylocoris flavipes(Hemiptera:Anthocoridae)in southern China

Grain & Oil Science and Technology 2021年第1期4卷 26-32页

作者： Zonglin Wu Shaohua Lu Jiying Li Shiyuan Miao Yujie Lu School of Food Science and Technology Henan University of TechnologyZhengzhou 450001China School of Grain Science and Technology Jiangsu University of Science and TechnologyZhenjiang 212003China

During the investigation of natural enemy insects in grain depots,we've found an Anthocoridae insect accounted for a high proportion of natural enemy *** identify the enemy insects from morphology and molecular biology,so as to realize more accurate and effective control of storage pests by using the enemy *** images of the insects under the ultra-depth microscope(VHX-5000)showed that the insect adults were black brown in colour;the antennas of both the female and male had four segments,which increase in length sequentially from the base to the end,and had a length of 756.2μm for the female and 741.1μm for the *** rostrums of both the female and the male insects were composed of three segments,the male's rostrum(596.8μm)were shorter than the female's(734.6μm),and the former two segments of the female's rostrum were significantly longer than the male'*** abdomen of the insect was bilaterally symmetrical,the female's abdomen was wider than the male,and the male insect had a dent between its eighth and ninth segments of the left abdomen,in which its male genitalia was *** addition,the nymphs as a whole were tangerine in colour,and the eggs were elliptic with creamy white gloss and gridding patterns on the *** cytochrome C oxidase I(COI)gene sequences of the insects were identified by DNA barcoding technique,and the determined COI sequences were then submitted to the Genbank database for Blast alignment,and the results showed that the COI gene sequences had a high degree of similarity(99%)with *** records in the Genbank ***,we could determine that the insect was *** addition,the neighbor joining(NJ)was used to construct the evolutionary tree,and 1,000 repeated tests were conducted on the confidence levels of each *** on the phylogenetic tree,we've found that *** belongs to xylocoris *** on morphology and COI sequence identification,we confidentiy determined that the collected nat

关键词： xylocoris flavipes Morphology DNA barcoding Stored products insects COI

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