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Anti-breast cancer effects and mechanisms of xihuang pill on human breast cancer cell lines

Journal of Traditional Chinese Medicine 2013年第6期33卷 770-778页

作者： Guofeng Pan Wandan Wang Lan Wang Fangbo Zhang Xiaojie Yin Jianlu Wang Rixin Liang Department of Traditional Chinese Medicine Beijing Shijitan Hospital Affiliated to Capital Medical University School of Traditional Chinese Medicine Capital Medical University Pharmacology and Pharmacokinetics Laboratory Institute of Chinese Material Medical China Academy of Chinese Medical Sciences

OBJECTIVE： To investigate the anti-breast cancer （BC） effects and mechanisms of action of xihuang pill （XHP） by conducting in vitro experiments on hu- man BC cell lines. METHODS： Two human BC cell lines （MCF-7 and MDA- MB231） were cultured and treated with XHP. Cell viability was detected using the 3-（4, 5-Dimeth- ylthiazol-2-yl）-2, 5-diphenyltetrazolium bromide （MTT） assay. Flow cytometry was used to measure the cell cycle and apoptosis. The cell cycle was ana- lyzed with propidium iodide staining. Apoptosis was evaluated using the Annexin V-fluorescein iso- thiocyanate/propidium iodide method. Western blotting was used to analyze the expression of es- trogen receptor （ER）-α and ***： XHP had growth-inhibitory effects on MCF-7 and MDA-MB231 cells with a half-maximal inhibitory concentration （IC50） of 10.14 mg/mL （MCF-7） and 8.98 mg/mL （MDA-MB231）. Apoptosis was induced to some extent. Certain changes in the ER were caused. Upregulation of ER-a protein was found in MCF-7 cells. ER-β expression in MDA-MB231 cells was increased. Cell-cycle arrest was not observed in the two BC cell lines. ER-β ex- pression in MCF-7 cells was unchanged. No ER-a ex- pression was shown in MDA-MB231 cells. CONCLUSION： These data suggest that XHP can af- fect cell viability and cause apoptosis, but that the cell cycle is not blocked. XHP has a certain impact on ER expression, but its mechanisms of action of anti-13C effects may not be due to regulation of ER expression.

关键词： Breast neoplasms Inhibitor of apopto-sis proteins Cell cycle Apoptosis Estrogen recep-tor alpha xihuang pill

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xihuang pill(西黄丸) Induces Mesenchymal-Epithelial Transition and Inhibits Loss of Apical-Basal Polarity in Colorectal Cancer Cell through Regulating ZEB1-SCRIB Loop

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Chinese Journal of Integrative Medicine 2014年第10期20卷 751-757页

作者：王邈孟静岩何俗非 College of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine Scientific Research Department Tianjin University of Traditional Chinese Medicine

Objective：To investigate the antiproliferative and anti-metastasis effect of xihuang pill（西黄丸,XP） on human colorectal cancer cell and to explore the molecular mechanism by which it produces the ***：Highly metastatic human colorectal cancer cell line LoVo was treated with low-,medium-,and highdose XP-containing serum（XP-L,XP-M,XP-H） groups for 48 h,cells intervened with no drug rat serum and PD98059[extracellular signal-regulated kinase（ERK） inhibitor]as negative and positive controls（NC and PC）*** proliferation assay was made using cell counting kit-8（CCK8）.The 8 μm pore-size transwell chamber and 4＇,6-diamidino-2-phenylindole（DAPI） staining were applied to examine the ability of invasion and migration of the *** protein expression of ERK1/2,zinc finger E-box-binding homeobox 1（ZEB1）,Scrib and lethal giant larvae homolog 2（Lgl2） was detected by Western blotting while the relative mRNA quantity of E-cadherin,N-cadherin,Occludin and junctional adhesion molecule-1（JAM1） was measured by realtime fluorescent quantitative polymerase chain reaction（RT-qPCR）.Results：XP induced a dose-dependent suppression on the proliferation of LoVo cells（P〈0.05 or P〈0.01）,with the inhibition rates varied from 27.30%to31.08%.Transwell assay showed that when preprocessed with PD98059 and XP-containing serum,the number of cells that passed the filter decreased significantly compared with that of NC group（P〈0.05 or P〈0.01）.Moreover,XP inhibited the protein expression of ERK1/2 and ZEB1（P〈0.05）;and up-regulated the protein expression of Scrib and Lgl2（P〈0.05）.The mRNA levels of E-cadherin,Occludin and JAM1 of the XP intervened groups and PC group markedly ascended（P〈0.05） while that of N-cadherin showed a descending tendency（P〉0.05）.Conclusion：XP intervention suppressed the ability of proliferation,invasion and migration of the LoVo *** ZEB1-SCRIB Loop so as to recover epithelial phenotype and apical junctional complex might be one of the mechanisms by wh

关键词： xihuang pill anti-metastasis epithelial-mesenchymal transition apical-basal polarity colorectal cancer

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Network pharmacology analysis combined with experimental verification of the molecular mechanism of xihuang pill in treating liver cancer

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Traditional Medicine Research 2023年第6期8卷 24-32页

作者： Meng-Xin He Ayesha T.Tahir Saba Waris Wen-Bo Cheng Jun Kang School of Life Sciences Tianjin UniversityTianjin 300072China Department of Biosciences COMSATS University IslamabadIslamabad 45550Pakistan Department of Gynacology Allama Iqbal Medical CollegeLahore 54000Pakistan Tianjin Key Laboratory of Medical Mass Spectrometry for Accurate Diagnosis Tianjin 300399China

Background:xihuang pill is a kind of traditional Chinese medicine,which has been widely used in the treatment of kinds of ***,there is still a lack of systematic understanding of the molecular mechanism of xihuang pill in the treatment of liver *** this work,we aim to explore the molecular mechanism of xihuang pill in treating liver ***:The functional components in xihuang pill were collected from Traditional Chinese Medicine Database and Analysis *** target genes of these components were also collected using Traditional Chinese Medicine Database and Analysis *** target genes of liver cancer were predicted using GeneCards *** intersecting genes were then analyzed with Venn *** Encyclopedia of Genes and Genomes and Database for Annotation,Visualization,and Integrated Discovery were used to analyze the ***,cell counting kit-8 was used to measure the half-maximal inhibitory concentration of xihuang *** living dead cell staining method was used to observe the survival of ***2 cell apoptosis was tested by flow cytometry with fluorescein isothiocyanate/propidium iodide double staining method,and then the mitochondrial damage was also detected by flow *** expression of target genes was detected by quantitative real-time polymerase chain ***:A total of 130 compounds and 198 genes were identified as potential active ingredients and putative liver cancer‑related *** obtained 1,899 disease targets and 297 transcriptome targets from the *** drug-disease intersecting genes,CCNB1,BIRC5,TOP2A,ESR1,IGF2 and IGFBP3 were *** are enrichment in apoptosis,PI3K-AKT signaling pathway,MAPK signaling pathway,pathways in cancer and p53 signaling ***,it was found that the apoptosis rate of the HepG2 cells in xihuang pill treated group was significantly higher than that of the control *** the apoptosis rate gradually increased in a dose depe

关键词： xihuang pill liver cancer network pharmacology p53 signal pathway apoptosis-multiple species pathway

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