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In Vivo Confocal Microscopic Observation of Lamellar corneal Transplantation in the Rabbit Using xenogenic acellular corneal scaffolds as a Substitute

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Chinese Medical Journal 2015年第7期128卷 933-940页

作者： Yun Feng Wei Wang Department of Ophthalmology Peking University Third Hospital Peking University Eye Center Beijing 100191 China

Background： The limiting factor to corneal transplantation is the availability of donors. Research has suggested that xenogenic acellular corneal scaffolds （XACS） may be a possible alternative to transplantation. This study aimed to investigate the viability of performing lamellar corneal transplantation （LCT） in rabbits using canine XACS. Methods： Fresh dog corneas were decellularized by serial digestion, and LCT was performed on rabbit eyes using xenogeneic decellularized corneal matrix. Cellular and morphological changes were observed by slit-lamp, light, and scanning electron microscopy at 7, 30 and 90 days postoperatively, lmmunocytochemical staining for specific markers such as keratin 3, vimentin and MUC5AC, was used to identify cells in the graft. Results： Decellularized xenogenic corneal matrix remained transparent for about l-month after LCT. The recipient cells were able to survive and proliferate into the grafts. Three months after transplantation, grafts had merged with host tissue, and graft epithelialization and vascularization had occurred. corneal nerve fibers were able to grow into the graft in rabbits transplanted with XACS. Conclusions： xenogenic acellular corneal scaffolds can maintain the transparency of corneal grafts about 1-month and permit growth of cells and nerve fibers, and is, therefore, a potential substitute or carrier for a replacement cornea.

关键词： corneal Substitute Decellularization Lamellar corneal Transplantation xenogenic acellular corneal scaffolds

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