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Isolation and characterization of rice lesion mimic mutants from a T-DNA tagged population

Progress in Natural Science:Materials International 2005年第1期 18-24页

作者： LI Shutian 1,2, PEI Zhongyou 1, LUO Lijuan 3, TIAN Yingchuan 1 and HE Chaozu 1* (1. National Key Laboratory of Plant Genomics, Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China 3. College of Agronomy, South China University of Tropical Agriculture, Danzhou 571737, China)

A rice (Oryza sativa L. ssp. japonica cv. Nipponbare) T-DNA tagged population consisting of about 7000 individual lines was generated and screened for rice lesion mimic mutants in the T 1 generation. Ten lines were found to develop spontaneous lesions in the absence of pathogen infection and displayed distinct lesion phenotypes. These mutants were tentatively designated as lm1—lm10 (for lesion mimic), respectively. Lesion formation of lm mutants was developmentally regulated, and all the mutants showed stunted growth and reduced fertility. Genetic analysis demonstrated that all the mutations were recessive, and five partially fertile mutants (lm4—lm8) were derived from different loci. Mimic lesions occurring on the leaves of lm mutants resulted from cell death as revealed by trypan blue staining. Six of them (lm3—lm8) exhibited enhanced resistance to five bacterial blight isolates, indicating their wide-spectrum resistance to this pathogen. These results imply that some lesion mimic mutations of rice might be involved in disease resistance signaling pathways, and that isolation of these mutated genes may be useful for elucidating molecular mechanisms of plant disease resistance. Among the mutants, only one mutant, lm6, was preliminarily shown to cosegregate with the inserted T-DNA in its T 1 generation, making it feasible to isolate the gene responsible for the phenotype of this mutant.

关键词： T-DNA tagging lesion mimic hypersensitive response xanthomonas oryzae pv. oryzae Oryza sativa.

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