检索结果-南通市图书馆

Asian Pacific Journal of Tropical Medicine 2014年第7期7卷 505-512页

作者： Chakkaravarthy Arunkumar Pandurangan Pandiaraja P.R.Prince Perumal Kaliraj Centre for Biotechnology Anna University

Objective:To elucidate immunoprophylactic potential of recombinant wuchereria bancrofti(***)cuticular collagen(COL-4)in BALB/c mice and filarial clinical ***:col-4 gene was PCR amplified from *** L3 cDNA library and cloned in pRSET B *** COL-4 was over expressed in salt inducible system and was purified by nickel afiinity *** and cellular responses were measured by EUSA and peripheral blood mononuclear cells(PBMC)of various filarial clinical samples respectively using purified recombinant COL-4 *** the protective immune responses of COL-4 immunized BALB/c mice were ***:Sequence analysis of COL-4 with human host proteins reveals lack of *** recombinant COL-4 was found to be at 15 kDa fusion *** affinity purified COL-4 showed significant reactivity with putatively immune sere and in a similar fashion it demonstrated marked proliferation in PBMC *** studies in experimental filarial host(mice)elicited significant liters with protective antibody isotype profile(IgM and IgG).Cellular immune responses were also significant in terms of splenocytes proliferation assay on mice ***:Our immunological findings in experimental host suggest Th2mediated immune ***,we propose that *** COL-4 could be an efficacious vaccine candidate against lymphatic filariasis.

关键词： Lymphatic filariasis wuchereria banerofti Cuticular collagen Vaccine

Evaluation of PCR-ELISA as a tool for monitoring transmission of wuchereria bancrofti in District of Gampaha,Sri Lanka

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2013年第5期3卷 381-387页

作者： Asha Dilrukshi Wijegunawardana Nilmini Silva Gunawardane Chanditha Hapuarachchi Aresha Manamperi Kithsiri Gunawardena Wimaladharma Abeyewickrama Molecular Medicine Unit Faculty of MedicineUniversity of Kelaniya Environmental Authority Department of Parasitology Faculty of MedicineUniversity of Kelaniya

Objective:To compare wuchereria bancrofti(***)infection rates of Culex quinquefasciatus,using dissection and PCR-EUSA in two consecutive time periods(from 2007to 2008 and from 2008 to 2009).Methods:Mosquitoes were collected in 30 sentinel and 15 nonsentinel sites in 15 Medical Officer of Health areas of Gampaha District known for the presence of *** transmission in two consecutive time period of 2007 to 2008 and 2008 to *** mosquitoes were dissected to determine the *** larvae(L_1 L_2,L_3,).PCR was carried out using DNA extracted from mosquito pools(15 body parts/pool)utilizing the primers specific for Wb-Sspl *** products were analyzed by hybridization ELISA using fluorescein-labeled wild type specific *** prevalence of infected/infective mosquiloes in PCR pools(3 pools/site)was estimated using the PoolSereen^(TM)algorithm and a novel probability—based ***:Of 45 batches of mosquitoes dissected,*** infected mosquitoes were found in19 and 13 batches,with an infection rate of 13.29%and 3.10%with mean larval density of 8.7 and1.0 larvae per mosquito for two study periods in the Gampaha *** of 405 pools of head,thorax and abdomen were processed by PCR-EUSA for each *** these,51 and 31 pools were positive for *** in the two study periods *** association of dissection based prevalence rates with PCR based rates as determined by the Pearson correlation coefficient were0.176 and 0.890 respectively for the two ***:Data indicate that PCR-EUSA is more sensitive than the traditional dissection techniques for monitoring transmission intensity.

关键词： wuchereria bancrofti Culex quinquefasciatus Dissection PCR-ELISA Sri Lanka

Prevalence of filarial parasites in domestic and stray cats in Selangor State, Malaysia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2015年第9期8卷 693-697页

作者： Nazeh M Al-Abd Zurainee Mohamed Nor Mustafa Kassim Marzida Mansor Abdulelah H.Al-Adhroey Romano Ngui Sinnadurai Sivanandam Department of Parasitology Faculty of Medicine University of Malaya Department of Anesthesiology Faculty of Medicine University of Malaya

Objective: To determine the prevalence of the filarial parasites, ie., Brugia malayi, Brugia pahangi(B. pahangi), Dirofilaria immitis and Dirofilaria repens(D. repens)in domestic and stray cats. Methods: A total of 170 blood sample were collected from domestic and stray cats and examined for i larial worm parasites in two localities, Pulau Carey and Bukit Gasing, Selangor State, Malaysia. Results: The overall prevalence of infection was 23.5%(40/170; 95% CI=17.4–30.6). Of this, 35%(14/40; 95% CI=22.1–50.5) and 50%(20/40; 95% CI=35.2–64.8) were positive for single B. pahangi and D. repens, respectively. The remaining of 15%(6/40; 95% CI=7.1–29.1) were positive for mixed B. pahangi and D. repens. In addition, 75% of the infected cats were domestic, and 25% were strays. No Brugia malayi and Dirofilaria immitis was detected. Eighty-four cats were captured at Pulau Carey, of which 35.7%(30/84) were infected. Among the cats determined to be infected, 93%(28/30; 95% CI=78.7–98.2) were domestic, and only 6.7%(2/30; 95% CI=19.0–21.3) were strays. Conversely, the number of infected cats was three times lower in Bukit Gasing than in Pulau Carey, and most of the cats were stray. Conclusions: B. pahangi and D. repens could be the major parasites underlying i lariasis in the study area. Adequate prophylactic plans should be administrated in the cat population in study area.

关键词： Filarial parasites Brugia wuchereria Dirofilaria Cat Filariasis

Historic accounts of Mansonella parasitaemias in the South Pacific and their relevance to lymphatic filariasis elimination efforts today

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2016年第3期9卷 201-207页

作者： J.Lee Crainey Tullio Romao Ribeiro da Silva Sergio Luiz Bessa Luz Ecologia de Doencas Transmissiveis na Amazonia Instituto Leonidas e Maria Deane-Fiocruz Amazonia Rua Terezina

There are two species of filarial parasites with sheathless microfilariae known to commonly cause parasitaemias in humans:Mansonella perstans and Mtinsonella *** most contemporary accounts of the distribution of these parasites,neither is usually considered to occur anywhere in the Eastern ***,Sir Patrick Manson,who first described both parasite species,recorded the existence of sheathless sharp-tailed Mansonella ozzardilike parasites occurring in the blood of natives from New Guinea in each and every version of his manual for tropical disease that he wrote before his death in ***'s reports were based on his own identifications and were made from at least two independent blood sample collections that were taken from the *** region Mansonella perstans parasitaemias were also later(in 1923) reported to occur in New Guinea and once before this(in 1905) in *** Mansonella-parasilaernias are generally regarded as benign,they are thought to be of public health importance because they can affect the epidemiological monitoring of other filarial *** this article,we reviewed the historic literature concerning Pacific-origin Mansonella-parasitaemias in an attempt to explain how,despite repeated reports of Pacificregion Mansonella-parasilaemias,by as early as the 1970 s,the WHO had arrived at the presentday view that wuchereria bancrofti is the only cause of filarial parasitaemias in Papua New *** have also evaluated the evidence supporting the contemporary existence of Pacificarea parasitaemia-causing Mansonella parasites and assessed the relevance such parasites could have for present-day lymphatic filariasis elimination efforts in the region.

关键词： Mansonella ozzardi Mansonella perstans wuchereria bancrofti Papua New Guinea Lymphatic filariasis Global Programme to Eliminate Lymphatic Filariasis

Worm under the Skin—An Unusal Case of Filariasis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Surgical Science 2018年第4期9卷 153-156页

作者： Murali Krishna Jaya Manchanda Surgical Specialist Military Hospital Palampur India Pathologist Military Hospital Yol India

Background: Filarial disease has a very high prevalence in South East Asian region especially India. It commonly presents as an affliction of the lymphatic system like acute lymphangitis and chronic lymphedema. Presentation of lymphatic filariasis as a subcutaneous nodule is a rare but previously reported scenario. Aim: We discuss a case with incidental detection of filaria in a subcutaneous nodule following excision biopsy. Case Presentation: 20-year-old male patient presented with subcutaneous swelling in left lower chest. Undergoing excision biopsy for same and on histopathological examination was found to be an encysted filarial worm. Conclusion: Such rare presentation of filariasis has been reported earlier also and should be kept in mind.

关键词： Filariasis Subcutaneous wuchereria

Bancroftiasis among the mumuye of Northeastern Nigeria: Parasitological and clinical studies in Northern Taraba State

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Health 2013年第1期5卷 138-142页

作者： Jacqueline A. Badaki Oladele B. Akogun Nobert B. Molta Godwin Imandeh Department of Biological Sciences Federal University of Technology Yola Nigeria Department of Biological Sciences University of Jos Jos Nigeria Department of Environmental Biology Adekunle Ajasin University Akungba-Akoko Nigeria

The study reports the prevalence of bancroftiasis in three Local Government Areas (LGAs) of Taraba State, Nigeria. Night blood samples collected from one thousand and thirty one individuals were stained with haematoxylin and examined microscopically for the presence of Wu-chereria bancrofti. The overall prevalence of infection in the study area was high (21.2%) but mean microfilaria densities were generally low. Statistically, prevalence rate differed significantly (χ2d.f.2 = 10.498;P χ2d.f.1 = 1.385;P > 0.05) between sexes. Infection also did not statistically differ between age groups (χ2d.f.5 = 13.219;P > 0.05) even though infection rate increased steadily with age and reaching a peak at the 40 - 49 year age group. Lymphoe-dema was the commonest (M = 12.4%, F = 19.2%) clinical manifestation of bancroftian morbidity encountered in the survey. Overall, the findings indicate that bancroftian filariasis is a public health problem in Taraba state and there is an urgent need to establish an elimination programme with a view of halting transmission in the area.

关键词： wuchereria Bancrofti Morbidity Microfilaraemia Mumuye Northeast Nigeria