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Transcriptional Profiling of Gossypium Darwinii Genes Differentially Regulated in Response to Drought Stress

Transcriptional Profiling of Gossypium Darwinii Genes Differ...

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2014年中国作物学会学术年会

作者：陈浩东 Fang Liu Hejun Lu Xingxing Wang M Kashif Riaz Khan M Kashif Ilyas Zhongli Zhou Xiao-yan Cai Chun-ying Wang Yu-hong Wang Yuqiang Li Kunbo Wang State Key Laboratory of cotton Biology/cotton Research Institute Chinese Academy of Agricultural Sciences 湖南省棉花科学研究所 Nuclear Institute for Agriculture & Biology(NIAB) FaisalabadPakistan cotton Sciences Research Institute of Hunan/National Hybrid cotton Research Promotion Center

In this study,we employed RNA-seq to analyse the transcriptomes from leaves of *** seedlings with and without drought stress.A total of 86.7 million valid reads with average lengths of 95.79 bp were generated from the two samples and S8960 transcripts with length more than 500 bp were *** on sequence similarity search with known proteins these transcripts were annotated with COG,KEGG and GO functional *** to gene expression abundance RPKM Value we conducted differential expression analysis,obtained 19,165 differentially expressed genes(32.5%) between two samples,including 7,392 up regulated genes and 11,773 down regulated *** the analysis of the high abundance of gene expression,we found some major gene may be involved in mediating cotton Drought stress resistence biological *** implementation of *** drought stress transcriptome sequencing,on the one hand we can obtain a large amount of the cotton seed EST sequence to further enrich the EST sequence information in NCBI database,on the other hand the data will facilitate genes discovery,large scale drought stress response genes expression analysis and EST-SSR function markers development.

关键词： Transcriptomes Drought stress Gossypium Darwinii wild cotton

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Construction a high density genetic linkage map in interspecific population of ***

Construction a high density genetic linkage map in interspec...

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第六届全国动植物数量遗传学学术研讨会

作者： Haodong Chen Fang Liu M Kashif Riaz Khan M Kashif Ilyas Xingxing Wang Zhongli Zhou Xiao-yan Cai Chun-ying Wang Yu-hong Wang Yuqiang Li Kunbo Wang State Key Laboratory of cotton Biology/ cotton Research Institute Chinese Academy of Agricultural Sciences cotton Sciences Research Institute of Hunan/ National Hybrid cotton Research Promotion Center

Because of the genetic basis of the cultivated species is too narrow,resulting in cotton in terms of yield,quality or stress resistance breeding *** it is necessary to adopt a variety of approaches,in particular explore good genes from wild species of cotton to enrich the genetic diversity of germplasm resources of cotton in *** belongs to tetraploid wild cotton,which has many good characteristics like good fibre fineness,drought resistance and verticillium wilt *** there is not

关键词： Gossypium Darwinii wild cotton Genetic map

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