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Luminescent silver nanoclusters for efficient detection of adenosine triphosphate in a wide range of ph values

Chinese Chemical Letters 2020年第12期31卷 3117-3120页

作者： Meijia Liu Xiangling Ren Xin Liu Longfei Tan Hui Li Jing Wei Changhui Fu Qiong Wu Jun Ren Hongbo Li Xianwei Meng Experimental Center of Advanced Materials School of Materials Science&EngineeringBeijing Institute of TechnologyBeijing 100081China Laboratory of Controllable Preparation and Application of Nanomaterials Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China CAS Key Laboratory of Cryogenics Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

In this work,polymethacrylic acid(PMAA)-templated silver nanoclusters(Ag NCs)were developed as the fluorescent probe for the efficient and sensitive detection of adenosine triphosphate(ATP)in a wide range of ph values.The fluorescence intensity of the Ag NCs could keep stable with ph values ranging from2.5 to 9.3.The detection of ATP was based on the quenching of the fluorescent Ag NCs in the presence of ATP.The fluorescence quenching of the Ag NCs with increasing ATP concentration was studied at ph 2.5,4.5,7.0 and 8.5 which involved a wide ph environment in body fluids.The limit of detection(LOD)for ATP was as low as 0.1 mmol/L in an acidic environment with ph of 2.5 and all the linear correlation coefficients were satisfactory under wide-span ph values from 2.5 to 8.5.In addition,the sensitive determination of ATP was also achieved by adding copper ions(Cu^2+).The high selectivity and rapid detection process proved that the fluorescent probe had great potential to detect ATP in biological samples under different ph conditions.

关键词： Adenosine triphosphate Silver nanoclusters Copper ions wide ph environment Fluorescence detection

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