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作者： Fredy Agil Raynaldo 江苏大学

学位级别：硕士

由异常威克汉姆酵母(Botrytis cinerea)和互隔交链格孢(Alternaria alternata)引起的灰霉病和黑斑病是全球樱桃番茄的主要采后病害,造成了相当大的经济损失。一直以来,合成杀菌剂因其有效性和低成本而被广泛应用于对樱桃番茄采后病害的... 详细信息

由异常威克汉姆酵母(Botrytis cinerea)和互隔交链格孢(Alternaria alternata)引起的灰霉病和黑斑病是全球樱桃番茄的主要采后病害,造成了相当大的经济损失。一直以来,合成杀菌剂因其有效性和低成本而被广泛应用于对樱桃番茄采后病害的控制。然而,合成杀菌剂的有害性促使人们研究更安全的药物或者方式来控制水果采后病害。近年来,生物防治剂有望成为保护果实抵御病原菌和增加农作物收益的有效方式。本研究旨在探索拮抗酵母异常威克汉姆酵母(wickerhamomyces anomalus)防治樱桃番茄采后灰霉病和黑斑病的潜力以及相关的机制。该研究成果可为拮抗酵母在樱桃番茄采后病害防治中的实际应用提供理论参考。本研究结果如下:***以浓度依赖性方式抑制樱桃番茄果实灰霉病和黑斑病的生长。***有效减少了樱桃番茄在贮藏14天后的自然腐烂而不影响其质量参数。***在20°C和4°C的环境中都能够大量增殖和繁殖,表明***可以与樱桃番茄伤口或者表面的病原菌竞争营养和空间。此外,***在体外显著控制了***和***的孢子萌发和芽管伸长。***可增强樱桃番茄的防御相关酶的活性,包括多酚氧化酶(PPO)、过氧化物酶(POD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)、抗坏血酸过氧化物酶(APX)几丁质酶(CHI),降低樱桃番茄中丙二醛(MDA)的含量。3.基于转录组测序技术,***处理上调了在果实病害防御中具有重要作用的基因的表达。这些基因可能有助于增强樱桃番茄体内的酶活性,提高果实的营养价值,合成病害反应中的调控因子,增强采后果实的抗病能力和抗氧化能力。

关键词：樱桃番茄生物防治 wickerhamomyces anomalus 酶活性转录组学

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Optimization of wickerhamomyces anomalus Fermentation Conditions for Pectinase Production Based on Wet Processing of Arabica Coffee in Yunnan Province

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Agricultural Science & Technology 2022年第3期23卷 30-39页

作者： ZHANG Shu-zhu MENG Jin XUE Xiao-ran KONG De-ting TIE Li-ping HUANG Jia-xiong QIN Shi-wen HE Fei-fei SUO Yu-kai School of Agriculture Yunnan UniversityKunming 650000PRC Key Laboratory of Chemistry in Ethnic Medicinal Resources State Ethnic Affairs Commission and Ministry of EducationYunnan Minzu UniversityKunming 650000PRC Key Laboratory of Synthetic Biology of Ethnic Medicinal Endophyte-derived Natural Products State Ethnic Affairs CommissionYunnan Minzu UniversityKunming 650000PRC Institute of Tropical and Subtropical Economic Crops Yunnan Academy of Agricultural SciencesPu’er 665000PRC

In order to improve the pectin-degrading efficiency in wet processing of Arabica coffee in Yunnan, Box-Behnken design and single factor experiment were used to optimize the fermentation conditions of five pectinolytic wickerhamomyces anomalus strains from the fermentation broth of Arabica coffee in Baoshan, Yunnan during wet processing with pectase activity as an indicator. The results showed that the five strains all synthesized pectin lyase(PL), polygalacturonase(PG), and pectin methylesterase(PM).Among them, strain CAP5 had strong ability to produce PG and PL,while strain CAP4 secreted a large amount of PM. Under optimized conditions, the activity of PG, PL, and PM of the five strains came in at 250.17~411.20 U/mL, 12.98~16.55 U/mL, and 208.52~322.83 U/mL,respectively. The four factors of nitrogen source concentration,fermentation time, Mn2+ concentration, and pH value were optimized and the optimal pectinase-producing fermentation conditions for five strains were as follows: peptone 2.2 g/L, fermentation time 30 h, Mn2+ 1.5 mmol/L, and pH 4.3. After fermentation under the optimized conditions, the maximum PG activity of CAP5 amounted to 411.20 U/mL, 114.03% higher than that before ***, the PG activity of strains CAP3, CAP4, CAP8, and CAP10 increased by 86.74%, 114.55%, 65.79%, and 66.07%,respectively, and the activity of PL and PM of the five strains rose 150.35%~218.56% and 341.07%~418.52%, respectively. These findings suggested that W. anomalus strains could be used as coffee starter and had great potential for the lysis of pectin.

关键词： wickerhamomyces anomalus Coffee Wet processing Pectinase Box–Behnken design

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Study of the Action Mode of wickerhamomyces anomalus against Colletotrichum gloeosporioides

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Journal of Agricultural Science and Technology(B) 2016年第5期6卷 341-349页

作者： Luis Femando Zepeda-Giraud Dario Rafael Olicon-Hemandez Cinthya Martinez-Lopez Guadalupe Guerra-Sanchez Laboratorio de Bioqulmica y Biotecnologla de Hongos Bioquimica Microbiana Departamento de Microbiologia Escuela Nacionalde Ciencias Biologicas Instituto Politecnico National Carpio y Plan de Ayala S/N. Col. Casco de Santo Tomas Ciudad de Mexico CDMX CP 11340 Mexico

Colletotrichum gloeosporioides is the causal agent of anthracnose disease in fruits and vegetables, representing a global problem. The use of biocontrol agents has proved effective against fungal diseases in a wide variety of products. In this work, the antifungal activity of wickerhamomyces anomalus against C. gloeosporioides isolated from contaminated avocados was evaluated. The antagonism and volatile compound inhibition were measured on Petri dishes. In the mixed cultures, the mycelia damage was observed by scanning electron microscope （SEM）. Chitinase and glucanase production by the antagonism was quantified by the reducing sugars method, and biofilm formation was evaluated with 1% crystal violet. The yeast W. anomalus could reduce the growth of C. gloeosporioides up to 65% by direct antagonism and 10% by volatile compounds. The antagonist did not allow the conidia germination and mycelia growth in any of the tested formulations. SEM showed mycelial damage caused by W. anomalus. The antagonist showed adhesion to the mycelium by a polysaccharide biofilm. The presence of mycelium stimulated the hydrolytic enzyme production with the maximal activity of 21.4 U/mg for chitinases at 24 h and 10 U/mg for glucanases at 60 h. These results showed that W. anomalus used together different mechanisms to express its antifungal activity against C. gloeosporioides. This study might be the first report for this phytopathogen isolated from avocado fruits, which could represent an opportunity to establish biocontrol of diseases for this agricultural product.

关键词： Colletotrichum gloeosporioides wickerhamomyces anomalus biocontrol avocado

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Analysis of long non-coding RNAs and mRNAs in harvested kiwifruit in response to the yeast antagonist,wickerhamomyces anomalus

Analysis of long non-coding RNAs and mRNAs in harvested kiwi...

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中国植物病理学会2021年学术年会

作者： ZHAO Qianhua YANG Qiya WANG Zhenshuo SUI Yuan WANG Qi LIU Jia ZHANG Hongyin School of Food and Biological Engineering Jiangsu University Department of Plant Pathology MOA Key Lab of Pest Monitoring and Green ManagementCollege of Plant ProtectionChina Agricultural University Chongqing Key Laboratory of Economic Plant Biotechnology College of Landscape Architecture and Life Science/Institute of Special PlantsChongqing University of Arts and Sciences

Biological control utilizing antagonistic yeasts is an effective method for controlling postharvest *** non-coding RNAs（lncRNAs） have been found to be involved in a variety of plant growth and development processes,including those associated with plant disease *** the present study,the yeast antagonist,wickerhamomyces anomalus,was found to strongly inhibit postharvest blue mold（Pencillium expansum） and gray mold（Botrytis cinerea） decay of ***,lncRNA high-throughput sequencing and bioinformatics analysis was used to identify lncRNAs in ***-treated wounds in kiwifruit and predict their function based on putative target *** results indicate that lncRNAs may be involved in activating ethylene（ET）,jasmonic acid（JA）,abscisic acid（ABA）,auxin（IAA） and signal transduction pathways that regulate the expression of several transcription factors（WRKY72,WRKY53,BHLH167,BHLH162,ZIP3,AP2）.These transcription factors（TFs） then mediate the expression of downstream,defense-related genes（ZAR1,PAD4,CCR4,NPR4） and the synthesis of secondary metabolites,thus,potentially enhancing disease ***,by stimulating the accumulation of antifungal compounds such as phenols and lignin,the antifungal ability of kiwifruit was also *** study provides new information on the mechanism of disease resistance induced by *** in kiwifruit,as well as a new disease resistance strategy that can be used to enhance the defense response of fruit to pathogenic fungi.

关键词： Kiwifruit lncRNAs Postharvest disease Resistance wickerhamomyces anomalus

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