检索结果-南通市图书馆

Induced neuronal differentiation of neural stem cells by a combination of cytokines One-step versus two-step methods

引用

Neural Regeneration Research 2009年第11期4卷 814-821页

作者： Xiaoli Sun Xue Chen Tianyi Zhang Zhenglin Jiang Weiwei Lin Ying Yuan XiaodongWang Department of Histology and Embryology Medical School of Nantong University Nantong 226001 Jiangsu Province China Affiliated Hospital of Nantong University Nantong 226001 Jiangsu Province China Jiangsu Province Key Laboratory of Neuroregeneration Nantong 226001 Jiangsu Province China

BACKGROUND： A combination of basic fibroblast growth factor （bFGF）, platelet-derived growth factor （PDGF）, human heregulin-beta-1, beta-mercaptoethanol retinoic acid and forskolin has been reported to induce the differentiation of rat bone marrow stromal cells into myelinating Schwann-like cells. OBJECTIVE： To investigate the inducing effects of a combination of bFGF, PDGF, human heregulin-beta-1, beta-mercaptoethanol retinoic acid and forskolin on neural stem cell differentiation by one- and two-step methods. DESIGN, TIME AND SETTING： A cytobiology experiment was performed at the Department of Histology and Embryology, Medical School of Nantong University, and Jiangsu Province Key Laboratory of Neuroregeneration, China, between August 2005 and January 2007. MATERIALS： A total of 30 healthy Sprague Dawley rat embryos at gestational days 14-16 were selected, bFGF, PDGF, human heregulin-beta-t, beta-mercaptoethanol, retinoic acid, and forskolin were purchased from Sigma, USA. METHODS： Passage 3 rat neural stem cells were cultured by a one-step method in serum-free medium plus 10 ng/m/bFGF, 5 ng/mL PDGF, 200 ng/mL heregulin-beta-1,35 ng/mL all-trans retinoic acid, and 5 pmol/L forskolin or by a two-step method in serum-free medium plus 35 ng/m/ all-trans retinoic acid for 72 hours, followed by serum-free medium plus 10 ng/mL bFGF, 5 ng/mL PDGF, 200 ng/mL heregulin-beta-t and 5 μmol/L forskolin. The control condition consisted of 10% fetal bovine serum alone or 20 ng/mL bFGF alone. MAIN OUTCOME MEASURES： Differentiated cells were identified by immunocytochemical staining for microtubule associate protein-2 （MAP2） and St 00 protein. Geometric parameters and sodium ion currents of the differentiated cells were measured by image analysis and whole-cell patch-clamp techniques, respectively. RESULTS： Compared with the two-step culture method, neuronal-like cells exhibited longer processes and a similar appearance to mature neurons using the one-step method. The percentage of

关键词： neural stem cells differentiation neuron cytokines whole-cell patch-clamp

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Inhibitory Effects of Neferine on Na_v1.5 Channels Expressed in HEK293 cells

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2016年第4期36卷 487-493页

作者：王琛王换肖军花王嘉陵向继洲汤强 Department of Pharmacology School of Basic Medicine Tongji Medical College Huazhong University of Science and Technology Department of Pharmacology The Third Affiliated Hospital of Zhengzhou University

Neferine, a bisbenzylisoquinoline alkaloid in Lotus Plumule, was proved to have a wide range of biological activities. In the present study, using whole-cell patch-clamp technique, we investigated the effects of neferine on Nav1.5 channels that are stably expressed in HEK 293 cells. We found that neferine potently and reversibly inhibited Nav1.5 currents in a concentration dependent manner with a half-maximal inhibition（IC50） being 26.15 μmol/L. The inhibitory effects of neferine on Nav1.5 currents were weaker than those of quinidine at the same concentration. The steady-state inactivation curve was significantly shifted towards hyperpolarizing direction in the presence of 30 μmol/L neferine, while the voltage-dependent activation was unaltered. Neferine prolonged the time to peak of activation, increased the inactivation time constants of Nav1.5 currents and markedly slowed the recovery from inactivation. The inhibitory effect of neferine could be potentiated in a frequency-dependent manner. These results suggested that neferine can block Nav1.5 channels under the open state and inactivating state and it is an open channel blocker of Nav1.5 channels.

关键词： neferine Nay1.5 channel whole-cell patch-clamp HEK293 cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cross-talk between NMDA and GABA_A receptors in cultured neurons of the rat inferior colliculus

引用

Science China(Life Sciences) 2011年第6期54卷 560-566页

作者： CONG DanNi TANG ZhengQuan LI LongZhu HUANG YiNa WANG Jun CHEN Lin Chinese Academy of Sciences Key Laboratory of Brain Function and Diseases School of Life SciencesUniversity of Science and Technology of ChinaHefei 230027China Auditory Research Laboratory University of Science and Technology of ChinaHefei 230027China Department of Anatomy and Neurobiology Northeastern Ohio Universities Colleges of Medicine and PharmacyRootstownOH 44272USA

Neuronal ion channels of different types often do not function independently but will inhibit or potentiate the activity of other types of channels,a process called *** N-methyl-D-aspartate receptor (NMDA receptor) and the γ-aminobutyric acid type A receptor (GABAA receptor) are important excitatory and inhibitory receptors in the central nervous system,***,cross-talk between the NMDA receptor and the GABAA receptor,particularly in the central auditory system,is not well *** the present study,we investigated functional interactions between the NMDA receptor and the GABAA receptor using whole-cell patch-clamp techniques in cultured neurons from the inferior colliculus,which is an important nucleus in the central auditory *** found that the currents induced by aspartate at 100 μmol L-1 were suppressed by the pre-perfusion of GABA at 100 μmol L-1,indicating cross-inhibition of NMDA receptors by activation of GABAA ***,we found that the currents induced by GABA at 100 μmol L-1 (IGABA) were not suppressed by the pre-perfusion of 100 μmol L-1 aspartate,but those induced by GABA at 3 μmol L-1 were suppressed,indicating concentration-dependent cross-inhibition of GABAA receptors by activation of NMDA *** addition,inhibition of IGABA by aspartate was not affected by blockade of voltage-dependent Ca2+ channels with CdCl2 in a solution that contained Ca2+,however,CdCl2 effectively attenuated the inhibition of IGABA by aspartate when it was perfused in a solution that contained Ba2+ instead of Ca2+ or a solution that contained Ca2+ and 10 mmol L-1 BAPTA,a membrane-permeable Ca2+ chelator,suggesting that this inhibition is mediated by Ca2+ influx through NMDA receptors,rather than voltage-dependent Ca2+ ***,KN-62,a potent inhibitor of Ca2+/calmodulin-dependent protein kinase II (CaMKII),reduced the inhibition of IGABA by aspartate,indicating the involvement of CaMKII in this *** s

关键词： inferior colliculus N-methyl-D-aspartate receptor γ-aminobutyric acid type A receptor whole-cell patch-clamp cross-talk

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Downregulated GPR30 Expression in the Epileptogenic Foci of Female Patients with Focal Cortical Dysplasia Type Ⅱb and Tuberous Sclerosis Complex Is Correlated with 18F-FDG PET-CT Values

Downregulated GPR30 Expression in the Epileptogenic Foci of ...

引用

第十六届中国医师协会神经外科医师年会

作者： Zhongke Wang Kaixuan Huang Xiaolin Yang Kaifeng Shen Ling Yang Ruotong Ruan Xianjun Shi Miao Wang Gang Zhu Meihua Yang Chunqing Zhang Shengqing Lv Hui Yang Xiaotang Fan Shiyong Liu Department of Neurosurgery Xinqiao HospitalArmy Medical University (Third Military Medical University) Department of Developmental Neuropsychology School of PsychologyArmy Medical University (Third Military Medical University) Department of Basic Medical College Army Medical University (Third Military Medical University)

Focal cortical dysplasia type Ⅱb(FCDⅡb) and tuberous sclerosis complex(TSC) are typical causes of developmental delay and refractory epilepsy.G-protein-coupled receptor 30(GPR30) is a specific estrogen receptor that is critical in neurodevelopment,neuroinflammation,and neuronal excitability,suggesting that it plays a potential role in the epilepsy of patients with FCDⅡb and ***,we investigated the role of GPR30 in patients with FCDⅡb and *** found that the expression of GPR30 and its downstream protein kinase A(PKA) pathway were decreased and negatively correlated with seizure frequency in female patients with FCDⅡb and TSC,but not in male ***30 was widely distributed in neurons,astrocytes,and microglia,and its downregulation was especially notable in *** GPR30 agonist G-1 increased the expression of PKA and p-PKA in cultured cortical neurons,and the GPR30 antagonist G-15 exhibited the opposite effects of *** NF-κB signaling pathway was also activated in the specimens of female patients with FCDⅡb and TSC,and was regulated by G-1 and G-15 in cultured cortical *** also found that GPR30 regulated cortical neuronal excitability by altering the frequency of spontaneous excitatory postsynaptic currents and the expression of NR2A/***,the relationship between GPR30 and glycometabolism was evaluated by analyzing the correlations between GPR30 and F-FDG PET-CT values(standardized uptake values,SUVs).Positive correlations between GPR30 and SUVs were found in female patients,but not in male ***,GPR30 expression and SUVs were significantly decreased in the epileptogenic tubers of female TSC patients,and ROC curves indicated that SUVs could predict the localization of epileptogenic *** together,our results suggest a potential protective effect of GPR30 in the epileptogenesis of female patients with FCDⅡb and TSC.

关键词： G-protein-coupled receptor 30 Focal cortical dysplasia type Ⅱb Tuberous sclerosis complex whole-cell patch-clamp 18F-FDG Positron emission tomography-computerized tomography Standardized uptake values

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Sodium salicylate,an aspirin metabolite,lowers the excitability of the medial geniculate body

Sodium salicylate,an aspirin metabolite,lowers the excitabil...

引用

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Yan-Yan SU~1,Bin LUO~(1,*),Lin CHEN~(1,2,*) 1 Auditory Research Laboratory,School of Life Sciences,University of Science and Technology of China,Hefei 230027, China 2 CAS Key Laboratory of Brain Function and Disease,University of Science and Technology of China,Hefei 230027,China

＜正＞Previous studies suggest that sodium salicylate（NaSal）,an asparin metabolite,raises the neural excitability in the auditory cortex to cause *** the present study,we wanted to determine whether NaSal had a similar effect in the medial geniculate body（MGB）,a central auditory structure that provides the immediate inputs to the auditory *** whole-cell patch-clamp recordings from MGB slices in rats,we measured effects of NaSal（1.4 mmol/L） on neuronal intrinsic properties and the synaptic *** found that NaSa1 decreased the membrane input resistance,hyperpolarized the resting membrane potential,suppressed the current-evoked firing,changed action potential properties and depressed rebound *** also reduced the postsynaptic response evoked by stimulating the brachium of inferior colliculus（BIC）,indicating a reduction in efficacy of the synaptic transmission. Our results demonstrate that NaSal lowers the neural excitability via altering neuronal intrinsic properties and reducing the synaptic transmission in the MGB,which is distinct from its excitatory effects on the auditory *** suggest that NaSal-induced tinnitus may be partly due to the abnormal neural synchrony in the auditory cortex resulting from the reduced excitability of the MGB.

关键词： brain slice intrinsic property medial geniculate body salicylate synaptic transmission tinnitus whole-cell patch-clamp

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Contribution of presynaptic HCN channels in GABAergic substantia gelatinosa neurons from GAD67-GFP transgenic mice

Contribution of presynaptic HCN channels in GABAergic substa...

引用

中国神经科学学会第十二届全国学术会议

作者： Si-cong Peng Jing Wu Da-ying Zhang Chang-ning Xie Ling-hui Xiao Tao Liu Department of Pediatrics the First Affiliated Hospital of Nanchang University Department of Pain Clinic the First Affiliated Hospital of Nanchang University

Aim of Investigation: Hyperpolarization-activated cyclic nucleotide-gated（HCN） channels induced an inward Na+-K+ mixed currents（Ih） which is involved in the nociceptive transmission and neuropathic pain. The expression of Ih was found in spinal lamina II（also named substantia gelatinosa, SG） neurons, especially in the GABAergic neurons which labelled green fluorescent protein（GFP） in GAD67-GFP mice. To know in detail the role of HCN channels in regulating nociceptive transmission, we investigated the contribution of presynaptic HCN channels to excitatory and inhibitory inputs of GABAergic SG neurons. Methods: Juvenile（3-5 weeks） male GAD67-GFP C57/BL6 transgenic mice were used. whole-cell patch-clamp recordings were applied to SG neurons in transverse spinal cord slices to investigate the spontaneous excitatory and inhibitory postsynaptic currents（s EPSCs and s IPSCs）. Results: Consist with previous studies, 88.9%（n = 136/153 neurons） of recorded GFPlabelled SG neurons expressed Ih and the average amplitude was 217.9 ± 15.2 p A. Perfusion of 10 mM of ZD7288（an antagonist of HCN channel） did not affect the s EPSCs of GFP-labelled neurons（103 ± 5% of control, n = 9/9 neurons）. Neither did 50 mM of forskoin（113 ± 6% of control, n = 7/7 neurons）, an activator of adenylate cyclase. However, ZD7288 significantly decreased s IPSC frequency by 37 ± 6% but not affect their amplitude in 78%（n = 7/9 neurons） of GFP-labelled neurons. In the remaining two neurons, no detectable effects of ZD7288 on s EPSCs were found in one of them, but ZD7288 increased the s EPSC frequency by 45% in the other neuron. Consistently, forskolin dramatically increased s IPSC frequency by 64 ± 29% in 50%（n = 5/10 neurons） of GFP-labelled neurons. In the remaining five neurons, no detectable effects of forskolin on s EPSCs were found in two of them, but forskolin decreased the s EPSC frequency by 34 ± 6% in the other three neurons. Conclusions: The expression of the HCN channels is different between e

关键词： Hyperpolarization-activated cyclic nucleotide-gated channels substantia gelatinosa excitatory postsynaptic currents inhibitory postsynaptic currents GABAergic whole-cell patch-clamp

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Effects of sevoflurane on presynaptic inhibition of primary sensory neurons

Effects of sevoflurane on presynaptic inhibition of primary ...

引用

中国神经科学学会第十届全国学术会议

作者： Meng-Jie CHEN Ke-Tao MAO Jun-Qiang SI Chao FAN Li LI Department of anesthesiology Shihezi University Medical College The key Laboratory of Xinjiang Endemic and Ethnic Diseases Shihezi University Medical College Department of Physiology Wuhan University School of Basic Medical Sciences Department of Physiology Huazhong University of Science and Technology of Basic Medical Sciences

Sevoflurane,a kind of volatile anaesthetic of halogen family,has a higher aqueous solubility than others for more rapid uptake elimination and adjustment of depth,as well as more rapid recovery from anaesthesia and fewer *** characteristics make it an ideal inhaled anesthetic widely used in *** studies have shown that sevoflurane had significant antinociception at the spinal level.γ-Aminobutyric Acid（GABA） exists in the central nervous system,plays an important role in nociceptive information processing or pain modulation as one of the most abundant and important inhibitive *** root ganglion（DRG） neurons play an important role in the occurrence and maintaining of pain as the primary afferent neurons of somatosensory *** To investigate the effects of GABA-activated currents in acutely isolated dorsal root ganglion neurons in rats and discuss the analgesia mechanism of sevoflurane at the spinal cord *** Using the whole-cell patch-clamp technique to record the effects of GABA-activated currents in dorsal root ganglion ***（1） The GABA（1-1000μmol/L） can make 86.8%（92/106） of the DRG neurons produce concentration dependence of the inward currents.（2）The enhancement of the GABA-activated currents by sevoflurane was no concentration dependence,at 300 μmol/L was statistically significant difference（P ＜ 0.05,n=6）.（3） The enhancement of the GABA-activated currents by sevoflurane（100,300,1000 μmol/L） related to the given dose of GABA,when 10 μmol/L was the most obvious（P ＜ 0.05,n=6）.Conclusion Sevoflurane can enhance the GABA-activated currents in dorsal root ganglion neurons,and reduce the transmission of harmful information by enhance the presynaptic inhibition of primary sensory neurons at the spinal cord level.

关键词： Sevoflurane GABA dorsal root ganglion(DRG) whole-cell patch-clamp primary sensory

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Impact of inhibition of Qo and Qi site of mitochondrial complex Ⅲ on persistent sodium currents and neuron excitability in rat hippocampal CA1 cells

Impact of inhibition of Qo and Qi site of mitochondrial comp...

引用

中国神经科学学会第六届学术会议暨学会成立十周年庆祝大会

作者： Lai Bin, Zhang Li, Dong Yan-lian, Zhu Yan-hua, Sun Feng-yan, Zheng Ping State Key Laboratory of Medical Neurobiology, Fudan University Shanghai Medical College, Shanghai 200032,China

＜正＞Hypoxia-induced inhibition of Qo and Qi site of mitochondrial complex Ⅲunder hypoxia has received attention, but its downstream pathways remain unclear. In this paper, we used the Qo site inhibitor myxothiazol and the Qi site,inhibitor antimycin A to mimick the inhibition of the Qo and Qi site of mitochondrial complex Ⅲand studied the effects of the inhibition of these site on persistent sodium currents, transient sodium currents and neuron excitability in rat hippocampal CA1 cells with whole cell patch-clamp methods combined biochemical and pharmacological approaches. The results showed that:

关键词： hippocampus mitochondrial myxothiazol antimycin A persistent sodium currents transient sodium currents neuron excitability whole-cell patch-clamp protein kinase C reactive oxygen species

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The electrophysiological properties and mechanism of cholinergic regulation of the pyramidal neurons in the layer Ⅴ of the primary auditory cortex in developing mouse

The electrophysiological properties and mechanism of choline...

引用

中国生理学会内分泌代谢、比较生理与应激生理学术会议

作者： Hui-Juan JIA Wei-Wei Zheng Xin WANG Hubei Provincial Key Laboratory of genetic regulation and integrative biology at School of Life Sciences Central China Normal University

Objective Auditory cortex(AC) is the highest auditory center along the auditory pathway for coding and processing of acoustic *** layer Ⅴ(L5) of the primary auditory cortex(AI) plays an important role in processing and integrating acoustic *** vivo studies have shown that AI can undergo frequency-specific auditory plasticity through learning and experience,which modulated by the cholinergic system through muscarinic acetylcholine receptors(mAChR).The previous studies focus mainly on adult animals and there is a lack of studies on the intrinsic properties of AI-L5 pyramidal neurons in developing *** cellular mechanisms of mAChR mediated auditory plasticity are not ***,the present study explores the intrinsic properties of AI-L5 pyramidal neurons and the cellular mechanism of mAChR activation in developing *** whole-cell patch-clamp recording and pharmacological methods Results(1) The excitability of AI-L5 pyramidal neurons of developing mouse is gradually decrease in developing mouse form postnatal lOdays to postnatal 29 days(P10-P29)(2)The excitability of AI-L5 pyramidal neurons are significantly enhanced after activating mAChR.(3) The persistent activity(PA) was completely blocked after blocking muscarinic receptor Ml and M3,*** firing frequency of PA were significantly reduced(p < 0.01) after blocking muscarinic receptor M2,and there is no significant difference on the firing frequency of PA after blocking muscarinic receptor *** indicated that muscarinic receptors Ml,M2 and M3 are involved in generating PA,while muscarinic receptor M4 didn't involve in generating PA.

关键词： whole-cell patch-clamp primary auditory cortex plasticity persistent activity muscarinic receptor

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：