检索结果-南通市图书馆

Single-nucleus RNA sequencing reveals heterogeneity among multiple white adipose tissue depots

Life Metabolism 2023年第6期2卷 58-73页

作者： Limin Xie Wanyu Hu Haowei Zhang Yujin Ding Qin Zeng Xiyan Liao Dandan Wang Wanqin Xie Hannah Xiaoyan Hui Tuo Deng National Clinical Research Center for Metabolic Diseases and Department of Metabolism and EndocrinologyThe Second Xiangya Hospital of Central South UniversityChangshaHunan 410011China Key Laboratory of Diabetes Immunology Ministry of Educationand Metabolic Syndrome Research CenterThe Second Xiangya Hospital of Central South UniversityChangshaHunan 410011China The First Affiliated Hospital Department of OrthopedicsHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China NHC Key Laboratory of Birth Defect for Research and Prevention Hunan Provincial Maternal and Child Health Care HospitalChangshaHunan 410028China School of Biomedical Sciences The Chinese University of Hong KongHong Kong 999077China Clinical Immunology Center The Second Xiangya Hospital of Central South UniversityChangshaHunan 410011China

Regardless of its anatomical site,adipose tissue shares a common energy-storage role but exhibits distinctive properties.Exploring the cellular and molecular heterogeneity of white adipose tissue(WAT)is crucial for comprehending its function and properties.However,existing single-nucleus RNA sequencing(snRNA-seq)studies of adipose tissue heterogeneity have examined only one or two depots.In this study,we employed snRNA-seq to test five representative depots including inguinal,epididymal,mesenteric,perirenal,and pericardial adipose tissues in mice under physiological conditions.By analyzing the contents of main cell catego-ries and gene profiles of various depots,we identified their distinctive physiological properties.Immune cells and fibro-adipogenic progenitor cells(FAPs)showed dramatic differences among WAT depots,while adipocytes seemed to be conserved.The heightened presence of regulatory macrophages and B cells in pericardial adipose tissues implied their potential contribution to the preservation of coronary vascular function.Moreover,the selective aggregation of pericytes within mesenteric adipose tissue was likely associated with the maintenance of intestinal barrier homeostasis.Using a combination of RNA sequencing and snRNA-seq analysis,the major subpopulations of FAPs derived from these depots determined the site characteristics of FAPs to a certain extent.Our work estab-lishes a systematic and reliable foundation for investigating the heterogeneity of WAT depots and elucidating the unique roles these depots play in coordinating the function of adjacent organs.

关键词： white adipose tissue adipose tissue heterogeneity adipocyte subpopulations snRNA-seq

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Recent developments in natural products for white adipose tissue browning

引用

Chinese Journal of Natural Medicines 2020年第11期18卷 803-817页

作者： MA Peng HE Ping XU Chun-Yang HOU Bi-Yu QIANG Gui-Fen DU Guan-Hua State Key Laboratory of Bioactive Substance and Function of Natural Medicines Instiute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College and Bejing Key Laboratory of Drug Target and Screening ResearchBeijing 100050China Beijing Obstetrics and Gyecology Hospital Capital Medical UniversityBeijing 100026China

Excess accumulation of white adipose tissue(WAT) causes obesity which is an imbalance between energy intake and energy expenditure. Obesity is a serious concern because it has been the leading causes of death worldwide, including diabetes, stroke,heart disease and cancer. Therefore, uncovering the mechanism of obesity and discovering anti-obesity drugs are crucial to prevent obesity and its complications. Browning, inducing white adipose tissue to brown or beige(brite) fat which is brown-like fat emerging in WAT, becomes an appealing therapeutic strategy for obesity and metabolic disorders. Due to lack of efficacy or intolerable side-effects, the clinical trials that promote brown adipose tissue(BAT) thermogenesis and browning of WAT have not been successful in humans. Obviously, more specific means still need to be developed to activate browning of white adipose tissue. In this review, we summarized seven kinds of natural products(alkaloids, flavonoids, terpenoids, long chain fatty acids, phenolic acids, else and extract) promoting white adipose tissue browning which can ameliorate the metabolic disorders, including obesity, dislipidemia, insulin resistance and diabetes. Since natural products are important drug sources and the browning property plays a significant role in not only obesity treatment but also in type 2 diabetes(T2 DM) improvement, natural products of inducing browning may be an irreplaceable drug discovery orientation for obesity, diabetes and even other metabolic disorders.

关键词： Natural products Browning Brown adipose tissue white adipose tissue Obesity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Resistin-binding peptide antagonizes role of resistin on white adipose tissue

引用

Acta Pharmacologica Sinica 2007年第2期28卷 221-226页

作者： Nan GU~(2,3,4) Shu-ping HAN~(2,4) Li FEI~3 Xiao-qin PAN~3 Mei GUO~3 Rong-hua CHEN~3 Xi-rong GUO~(2,3,5)2 Department of Pediatrics,Nanjing Maternal and Child Health Hospital of Nanjing Medical University,Nanjing 210029,China 3 Pediatric Research Institute of Nanjing Medical University,Nanjing 210029,China Department of Pediatrics Nanjing Maternal and Child Health Hospital of Nanjing Medical University Nanjing China Pediatric Research Institute of Nanjing Medical University Nanjing China

Aim:To investigate the direct effects of resistin and resistin-binding peptide（RBP）on lipid metabolism and endocrine function in adipose tissue.Methods:Ratwhite adipose tissue was cultured in vitro and incubated for 24 h with 30 ng/mLrecombinant rat resistin protein（rResistin）or combined with RBP of varyingconcentrations(1×1-12mol/L,1×10-10mol/L,1×10-8mol/L).Free fatty acids（FFA）released into medium was measured by a colorimetric kit.The levels of proteinsecretion and mRNA expression of TNF-α and adiponectin were detected by ELISAkit and RT-PCR respectively.Results:The levels of FFA released into mediumwere significantly increased after 24 h of exposure to rResistin,but significantlydecreased after RBP was applied,although there was no difference between the 3concentrations.The protein level and gene expression of TNF-αin adipose tissuewere significantly increased after 24 h of exposure to rResistin,but only obviouslydecreased after incubated with 1×10-8mol/L RBP.The levels of protein secretionand mRNA expression of adiponectin in adipose tissue were significantly de-creased after 24 h of exposure to rResistin,but increased after incubated with RBPwith the higher concentrations.Conclusion:RBP can effectively antagonize therole of resistin on the lipid metabolism and endocrine function of adipose tissue.

关键词： resistin resistin-binding peptide white adipose tissue lipid metabolism endocrine function

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Hysteresis of white adipose tissue

引用

Open Journal of Endocrine and Metabolic Diseases 2015年第10期5卷 138-147页

作者： Alain Géloen Hédi Soula Lilas Hadji Emmanuelle Berger Université de Lyon CARMEN INSERM U1060 INSA-Lyon F-69621 Villeurbanne France IXXI Complex Systems Institute Lyon France EPI BEAGLE INRIA F-69621 Villeurbanne France

Objective: This study was performed to analyze the modifications within adipose tissue during calorie restriction and more specifically to state whether hysteresis occurs during fat mass reduction. Method: Rats male Wistar increased their body weight by 130 g under control conditions and were then submitted to a calorie restriction (CR) at 30% or 60% of control. Experiment has been stopped when the body weight of the group CR60% returned back to its initial value. Samples of retroperitoneal adipose tissue were collected by biopsies along the study. adipose cell size was analyzed using multisizer IV (Beckman Coulter) to determine the size distribution curves during natural growth and after calorie restriction. Results: After CR60%, body weights and adipose tissue masses were similar to the ones at the beginning of the experiment. adipose cell size distribution curve was shifted to the left compared to the one of initial control. adipose cell sizes were significantly lower after CR60% than those of control at the beginning of the experiment. Conclusions: These results state for the first time that hysteresis occurs in white adipose tissue after calorie restriction. The composition of adipose tissue after calorie restriction was significantly different than the one of initial control. After significant weight loss, organisms must be considered as different from the initial controls, they are most likely governed by different regulations which will have to be identified.

关键词： Adipocyte Size white adipose tissue Calorie Restriction Hysteresis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The local corticotropin-releasing hormone receptor 2 signalling pathway partly mediates hypoxia-induced increases in lipolysis via the c AMP–protein kinase A signalling pathway in white adipose tissue

The local corticotropin-releasing hormone receptor 2 signall...

引用

中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：熊艳蕾 Zhuan Qu Nan Chen Hui Gong Mintao Song Xuequn Chen Jizeng Du Chengli Xu 中国医学科学院基础医学研究所病理生理学系浙江大学医学院基础医学系神经生物学及生理学研究室

Our objective was to investigate the mechanisms by which the endogenous CRHR2 in white adipose tissue（WAT）regulates metabolic activities associated with lipogenesis and lipolysis under continuous exposure to hypoxia.We found that hypobaric hypoxia at a simulated altitude of 5,000m significantly reduced the body weight,food intake,and WAT mass of rats.Hypoxia also accelerated lipolysis and suppressed lipogenesis in WAT.Pretreatment with astressin 2B,a selective CRHR2 antagonist,partly but significantly attenuated the hypoxia-induced reductions in body weight and WAT mass by blocking the c AMP–protein kinase A（PKA）–hormone-sensitive lipase（HSL）/perilipin signalling pathway.Astressin 2B treatment failed to attenuate hypoxia induced lipogenic inhibition.In conclusion,activation of endogenousWAT Ucn2/3 autocrine/paracrine pathway was involved in hypoxia induced lipolysis via CRHR2-c AMP–PKA signalling pathway.This study provides the novel understanding of local CRHR2 signaling pathway playing important role in WAT loss and lipid metabolism under hypoxia.

关键词： Hypoxia Corticotropin-releasing hormone receptor 2 white adipose tissue Lipolysis

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Supplementation of branched-chain amino acids in protein-restricted diets modulates the expression levels of amino acid transporters and energy metabolism associated regulators in the adipose tissue of growing pigs

引用

Animal Nutrition 2016年第1期2卷 24-32页

作者： Yinghui Li Hongkui Wei Fengna Li Shuai Chen Yehui Duan Qiuping Guo Yingying Liu Yulong Yin Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central Ministry of AgricultureHunan Provincial Engineering Research Center of Healthy LivestockKey Laboratory of Agro-Ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of Sciences University of Chinese Academy of Sciences College of Animal Sciences Huazhong Agricultural University Hunan Co-Innovation Center of Animal Production Safety (CICAPS) Changsha Lvye Biotechnology Limited Company Academician Expert Workstation Hang Zhou King Techina Limited Company Academician Expert Workstation

This experiment was conducted to investigate the effects of branched-chain amino acids(BCAA)supplemented in protein-restricted diets on the growth performance and the expression profile of amino acid transporters and energy metabolism related regulators in the white adipose tissue(WAT)of different regional depots including dorsal subcutaneous adipose(DSA) and abdominal subcutaneous adipose(ASA), A total of 24 crossbred barrows(7.40 ± 0.70 kg) were randomly divided into 4 groups and were fed the following isocaloric diets for 33 days: 1) a recommended adequate protein diet(AP, 20% CP, as a positive control); 2) a low protein diet(LP, 17% CP); 3) the LP diet supplemented with BCAA(LP + B, 17% CP) to reach the same level of the AP diet group; 4) the LP diet supplemented with 2 times the amount of BCAA(LP + 2B, 17% CP). The daily gain and daily feed intake of the LP diet group were the lowest among all the treatments(P 0.05). Moreover, BCAA supplementation down-regulated the expression levels of amino acid transporters including L-type amino acid transporter 1 and sodium-coupled neutral amino acid transporter 2 in DSA, but up-regulated the expression level of Ltype amino acid transporter 4 in ASA(P < 0.05), Meanwhile, the energy sensor AMP-activated protein kinase α was activated in the DSA of pigs fed LP diet and in the ASA of the pigs fed AP or LP + 2B diets(P < 0.05). The mRNA expression profile of the selected mitochondrial component and mitochondrial biogenesis associated regulators in DSA and ASA also responded differently to dietary BCAA supplementation. These results suggested that the growth performance of growing pigs fed protein restricted diets supplemented with BCAA could catch up to that of the pigs fed AP diets. The res

关键词： Branched-chain amino acid Protein-restricted diet Energy metabolism white adipose tissue Pig

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ablation of steroid receptor coactivator-3 in mice impairsadipogenesis and enhances energy expenditure

引用

Frontiers of Medicine 2010年第2期4卷 229-234页

作者： Ling-Yan XU Xin-Ran MA Jie-Li LI Xiao-Ying LI Jian-Ming XU Shu WANG Guang NING Laboratory of Endocrinology and Metabolism Institute of Health SciencesShanghai Institutes for Biological SciencesChinese Academy of Sciences&Shanghai Jiao Tong University School of MedicineShanghai 200025China Shanghai Clinical Center for Endocrine and Metabolic Diseases Department of Endocrinology and MetabolismRui-Jin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Department of Molecular and Cellular Biology Baylor College of MedicineOne Baylor PlazaHoustonTX 77030USA

Obesity is a medical condition in which excess body fat has accumulated to an extent and may have an adverse effect on health,leading to reduced life expec-tancy,impaired energy homeostasis and increased health problems.The p160 steroid receptor coactivator(SRC)gene family members have been suggested to be involved in energy homeostasis,but the impact of SRC-3 ablation on white and brown adipose tissue needs to be elucidated.In the current study,we collected in vivo data and carried out morphological studies on the effect of SRC-3 deﬁciency on white adipose tissue(WAT)and brown adipose tissue(BAT).Primary cells were cultured to investigate the differentiation ability of both adipocytes.Western blot was applied to detect the expression of master genes governing adipogenesis and thermogenesis.We observed that SRC-3^(–/–)mice were lean,with reduced WAT and decreased serum leptin levels,mainly due to the smaller white adipocyte size caused by impaired adipo-genesis,presented by decreased peroxisome proliferator activated receptor g(PPARg)expression.In the BAT,the lipid droplets decreased signiﬁcantly in SRC-3^(–/–)mice as demonstrated by histological analysis and electron micro-scopic observation,which could be explained by enhanced thermogenesis.The expression of thermogenic marker gene PPARg coactivator 1αand uncoupling protein-1 increased in BAT of SRC-3^(–/–)mice,which proved our observations.Collectively,these results demonstrate that SRC-3 plays a key role in adipogenesis and energy expenditure.

关键词： steroid receptor coactivator-3 white adipose tissue brown adipose tissue obesity adipocytes energy expenditure

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：