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Rapid Detection of wheat blast Pathogen Magnaporthe oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Technology

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Engineering 2021年第9期7卷 1326-1335页

作者： Houxiang Kang Ye Peng Kangyu Hua Yufei Deng Maria Bellizzi Dipali Rani Gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson Yilin Zhou Xueping Zhou Md Tofazzal Islam Guo-Liang Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Pathology The Ohio State UniversityColumbusOH 43210USA Institute of Biotechnology and Genetic Engineering(IBGE) Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityDhaka 1706Bangladesh Centro de Ciências Agrárias Universidade Federal de São CarlosArarasSP CEP 13600-000Brazil Foreign Disease-Weed Science Research Unit(FDWSRU) United States Department of Agriculture-Agricultural Research Service(USDA-ARS)Ft.DetrickMD 21702USA

wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and *** MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is *** develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)*** polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and *** test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR *** this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 *** activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)*** then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat *** novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field.

关键词： wheat blast Magnaporthe oryzae Triticum Cas12a Nucleic acid rapid lateral flow immunoassay Field detection

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Medicinal Plants, A promising Source of Natural Fungicides against <i>Magnaporthe oryzae Triticum</i>, Causal Agent of wheat blast

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American Journal of Plant Sciences 2021年第5期12卷 748-758页

作者： Abul Hasnat Md. Shamim School of Agriculture and Rural Development Bangladesh Open University Gazipur Bangladesh

wheat blast, caused by a fungal pathogen, Magnaporthe oryzae Tritichum (MoT) pathotype (wheat isolates), was first reported in Bangladesh among Asian countries in 2016. It is a serious disease of wheat causing yield failures and significant economic losses during epidemic years. Extensive use of persistent synthetic pesticides to control notorious wheat blast imposes enormous threat to human health and environment. In addition, the increasing demand for organic food has stimulated people to look for alternative methods. Nowadays, the need for synthetic chemical-free agricultural practices is gaining importance due to effective in managing crop pests, inexpensive, biodegradable, easily available and have low toxicity to non-target organisms. In order to identify active plants, 12 kinds of methanol extracts obtained from 12 medicinal plants were conducted in vitro to test the effect against Magnaporthe oryzae Triticum (MoT) pathotype. The remarkable inhibitory activity {(29.6 ± 01.5) mm, (25.1 ± 01.0) mm and (20.0 ± 02.0) mm zone of inhibition)} exhibited by the extracts (5 mg/disk) obtained from Artemisia indica (Nagdona), Persicaria orientalis (Bishkatali) and Clerodendrum indicum (Bamonhati), respectively. Other medicinal plant extracts did not show any sign

关键词： Medicinal Plants Extracts Activity Fungicides Zone of Inhibition wheat blast

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Genetic sources and loci for wheat head blast resistance identified by genome-wide association analysis

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The Crop Journal 2022年第3期10卷 793-801页

作者： Lei Wu Xinyao He Muhammad Rezaul Kabir Krishna KRoy Md.Babul Anwar Felix Marza Yi He Peng Jiang Xu Zhang Pawan K.Singh CIMMYT-JAAS Joint Center for wheat Diseases Jiangsu Academy of Agricultural SciencesNanjing 210014JiangsuChina International Maize and wheat Improvement Center(CIMMYT) ApdoPostal 6-64106600 Mexico D.F.Mexico Bangladesh wheat and Maize Research Institute(BWMRI) NashipurDinajpur 5200Bangladesh Instituto Nacional de Innovación Agropecuaria y Forestal(INIAF) La PazBolivia

The emergence and spread of wheat blast caused by fungal pathogen Magnaporthe oryzae pathotype Triticum is a threat to global wheat *** resistance level and genetic loci for blast resistance in Chinese germplasm remain unknown.A panel of 266 bread wheat accessions from China,CIMMYTMexico and other countries was screened for head blast resistance under 12 field experiments in Bolivia and ***,a genome-wide association study was performed to understand the genetic basis of wheat blast *** average blast index of all the accessions was 53.7%±12.7%,and 10 accessions including Chinese accessions Yumai 10 and Yu 02321 showed moderate to high levels of blast resistance,accounting for only 3.8%in the ***-eight significant SNPs clustered in a 28.9 Mb interval on the 2 AS/2 NS translocation region,explaining phenotypic variation between10.0%and 35.0%.The frequency of the 2 AS/2 NS translocation in the Chinese accessions was as low as4.5%.These results indicated that the 2 NS fragment was the only major locus conferring resistance to wheat blast in this panel,and the resistant and moderately resistant lines identified could be deployed in breeding.

关键词： Magnaporthe oryzae pathotype Triticum Host resistance wheat blast GWAS

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Phenotypic and Molecular Assessment of wheat Genotypes Tolerant to Leaf Blight,Rust and blast Diseases

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Phyton-International Journal of Experimental Botany 2021年第4期90卷 1301-1320页

作者： Ashraful Alam Milan Skalicky Muhammad Rezaul Kabir Monwar Hossain Abdul Hakim Siddikun Nabi Mandal Rabiul Islam Babul Anwar Akbar Hossain Fahmy Hassan Amaal Mohammadein Muhammad Aamir Iqbal Marian Brestic Mohammad Anwar Hossain Khalid Rehman Hakeem Ayman EL Sabagh Bangladesh wheat and Maize Research Institute(BWMRI) Dinajpur5200Bangladesh Department of Botany and Plant Physiology Faculty of AgrobiologyFood and Natural ResourcesCzech University of Life Sciences PragueKamycka 129Prague16500Czechia Regional Agricultural Research Station BWMRIJashore7400Bangladesh Department of Biology College of ScienceTaif UniversityTaif21944Saudi Arabia Department of Agronomy Faculty of AgricultureUniversity of Poonch RawalakotHajira RoadShamsabad12350Pakistan Department of Plant Physiology Slovak University of AgricultureNitraTr.A.Hlinku 2Nitra94901Slovak Republic Department of Genetics and Plant Breeding Bangladesh Agricultural UniversityMymensingh2202Bangladesh Department of Biological Sciences Faculty of ScienceKing Abdulaziz UniversityJeddah21589Saudi Arabia Department of Agronomy Faculty of AgricultureKafrelsheikh UniversityKafrelsheikh33516Egypt

Globally among biotic stresses,diseases like blight,rust and blast constitute prime constraints for reducing wheat productivity especially in *** sustainable productivity,the development of disease-resistant lines and high yielding varieties is vital and *** study was conducted using 122 advanced breeding lines of wheat including 21 varieties developed by Bangladesh wheat and Maize Research Institute with aims to identify genotypes having high yield potential and resistant to leaf blight,leaf rust and blast *** genotypes were evaluated for resistance against leaf blight and leaf rust at Dinajpur and wheat blast at Jashore under field *** of 122 genotypes tested,20 lines were selected as resistant to leaf blight based on the area under the diseases progress curve under both irrigated timely sown and irrigated late sown ***-two genotypes were found completely free from leaf rust infection,59 genotypes were identified as resistant,and 13 genotypes were identified as moderately resistant to leaf *** genotypes were immune against wheat blast,42 genotypes were categorized as resistant,and 26 genotypes were identified as moderately resistant to wheat *** data revealed that the 16 genotypes showed a positive 2NS segment among the 18 immune genotypes selected against wheat blast under field *** genotypes BAW 1322,BAW 1295,and BAW 1203 can be used as earlier maturing genotypes and the genotypes BAW 1372,BAW 1373,BAW 1297 and BAW 1364 can be used for lodging tolerant due to short plant *** genotypes WMRI Gom 1,BAW 1349 and BAW 1350 can be selected for bold grain and the genotypes WMRI Gom 1,BAW 1297,BAW 1377 can be used as high yielder for optimum seeding condition but genotypes BAW 1377 and BAW 1366 can be used for late sown *** selected resistant genotypes against specific diseases can be used in the further breeding program to develop wheat varieties having higher disea

关键词： wheat blast leaf blight leaf rust 2NS marker grain yield

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Integrated pest management programme for cereal blast fungus Magnaporthe oryzae

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Journal of Integrative Agriculture 2022年第12期21卷 3420-3433页

作者： ZHANG Hai-feng Tofazzal ISLAM LIU Wen-de Department of Plant Pathology College of Plant ProtectionNanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests of Ministry of EducationNanjing 210095P.R.China Institute of Biotechnology and Genetic Engineering Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur 1706Bangladesh State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Magnaporthe oryzae,the causal agent of blast diseases,is a destructive filamentous fungus that infects many plants including most economically important food crops,rice,wheat,pearl millet and finger *** oryzae has numerous pathotypes because of its high host-specificity in the *** Oryza pathotype(MoO)of *** is the most devastating pathogen of rice,causing 10–30%yield loss in the *** the other hand,the Triticum pathotype(MoT)causes blast disease in wheat,which is now a serious threat to wheat production in some South American countries,Bangladesh and *** of low fungicide efficacy against the blast diseases and lack of availability of resistant varieties,control of rice and wheat blast diseases is ***,an integrated management programme should be adopted to control these two diseases in the ***,we introduced and summarized the classification,geographical distribution,host range,disease symptoms,biology and ecology,economic impact,and integrated pest management(IPM)programme of both rice and wheat blast diseases.

关键词： rice blast wheat blast Magnaporthe oryzae integrated pest management

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