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Ammonium Oxidizing Bacteria Activity and Nitrification Rate in Oil Contaminated wetland soil under Remediation with Nutrient Supplements and Leguminous Plants

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Journal of Environmental Protection 2016年第4期7卷 545-553页

作者： Richard C. John Emem S. Ntino Joseph P. Essien Department of Microbiology University of Uyo Uyo Nigeria

Previous investigation on the impact of crude oil on the growth of tropical legumes and its effect on nitrogen dynamics in wetland ultisol showed that oil contamination reduced N uptake by plants but increased N accumulation in soil microbial biomass. Moreover, the presence of hydrocarbons widened the C/N ratio in soil and led to more available N being immobilized by soil microorganisms. The present study was carried out to evaluate the activity of ammonium oxidizing bacteria (AOB) and their nitrification potential rate (NPR) in wetland soil under a remediation course. Mineralization studies showed that ammonium-N levels decreased while nitrate-N increased progressively in the uncontaminated soil (control) cultivated with leguminous plants (cover crops) during the 12 weeks remediation period. However, the remediated soils were affected in different ways. The experimented soil cultivated with Centrosema pubescens had higher mineral nitrogen (NH4-N, NO3-N, NO2-N, Total N and P) than soil cultivated with Calopogonium mucunoides and Pueraria phaseoloides. AOB counts recorded were in the ranged, 2.25 × 102 - 2.66 × 105, 2.31 × 102 - 2.11 × 104 and 4.25 × 102 - 2.98 × 104 respectively. The highest NPR was found in uncontaminated soil (11.68 - 60.92 nmol N/g dry weight soil (DWS)) followed by soil treated with poultry manure (9.65 - 24.86 nmol N/g DWS/h), NPK (7.88 - 39.45 nmol N/g DWS/h) and in the oil-contaminated soil (0.11 - 1.87 nmol N/g DWS/h). The relations between NH4-N concentration and NPR in soil cultivated with Centrosema (r = 0.852), Calopogonium (r = 0.745) and Pueraria (r = 0.722) were positively significant at 95% confidence limit. Similarly the relations between AOB density and NPR for Centrosema (r = 0.654;P = 0.05), Calopogonium (r = 0.588;P = 0.05) and Pueraria (r = 0.518;P = 0.05) were significant. The findings imply that nitrification potential of crude oil- contam

关键词： wetland soil AOB Contamination Nitrification Crude Oil Ammonium

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Studies on the bioremediation Actions of p-chloroaniline on wetland soil

Studies on the bioremediation Actions of p-chloroaniline on ...

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2009 International Conference on Environmental Science and Information Application Technology

作者： Cheng Ding1 Zhicheng Zhang2 Chunsheng Yang1 Cheng Yao2 1 School of Chemistry and Biology Engineering Yancheng Institute of Technology Yancheng 224003 China 2 College of Sciences Nanjing University of Techology Nanjing 210009 China

Chloroanilines are toxic substances listed as typical pollutants of the *** photolysis in sunlight is a possible route for it *** photolysis rate constant of p-chloroaniline（p-CA）in pure water and in wetland soil（C）was about 0.1059d-1 and 31 2.98×10-d-with a half-life was 6.55d and *** rate coefficient of p-CA in sterilized（A）and Non-sterilized（B）was 8.347×10-3and 3.46×10-3,degradation half-life was 830.39d and ***,indicating that microorganisms would accelerate the the degradation of p-CA.A bacterium capable of growing with p-CA as sole carbon and energy sources was isolated from rhizosphere soil in phragmites wetland by enrichment culture *** degradation temperature and pH value for degradation were about 30℃and pH7.0,for 4d in minimal medium culture fluids at vaccination of 10%.

关键词： p-chloroaniline bioremediation wetland soil

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Organic carbon accumulation capability of two typical tidal wetland soils in Chongming Dongtan,China

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Journal of Environmental Sciences 2011年第1期23卷 87-94页

作者： Shiping Zhang Lei Wang Jiajun Hu Wenquan Zhang Xiaohua Fu Yiquan Le Fangming Jin College of Environmental Science and Engineering of Tongji University State Key Laboratory of Pollution Control and Resources Reuse Tongji University Shanghai 200092 China

We measured organic carbon input and content of soil in two wetland areas of Chongming Dongtan （Yangtze River Estuary） to evaluate variability in organic carbon accumulation capability in different wetland soils. Observed differences were investigated based on the microbial activity and environmental factors of the soil at the two sites. Results showed that the organic carbon content of wetland soil vegetated with Phragmites australis （site A） was markedly lower than that with P. australis and Spartina alterniflora （site B）. Sites differences were due to higher microbial activity at site A, which led to higher soil respiration intensity and greater carbon outputs. This indicated that the capability of organic carbon accumulation of the site B soils was greater than at site A. In addition, petroleum pollution and soil salinity were different in the two wetland soils. After bio-remediation, the soil petroleum pollution at site B was reduced to a similar level of site A. However, the culturable microbial biomass and enzyme activity in the remediated soils were also lower than at site A. These results indicated that greater petroleum pollution at site B did not markedly inhibit soil microbial activity. Therefore, differences in vegetation type and soil salinity were the primary factors responsible for the variation in microbial activity, organic carbon output and organic carbon accumulation capability between site A and site B.

关键词： Chongming Dongtan organic carbon accumulation wetland soil microbial activity

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Vertical characteristics of the Hani terrace paddyfield ecosystem in Yunnan,China

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Frontiers in Biology 2008年第3期3卷 351-359页

作者： Baoshan CUI Zheyuan YOU Min YAO School of Environment Beijing Normal UniversityBeijing 100875China State Key Joint Laboratory of Environmental Simulation and Pollution Control Beijing 100875China

The Hani terrace paddyfield in Yunnan Province,China is categorized as a‘constructed wetland’under the Lamsar Convention *** Hani terrace paddyfield ranges from an altitude of 144 to 2000 m above sea level(ASL)in the southern slopes of the Ailao Mountains,angling down at a range of 15°to 75°.In this study,we investigated the ecosystem of the terrace paddyfields in the Mengpin and Quanfuzhuang administrative villages located at the center of the cultural heritage conservation district in the Hani terrace *** Hani terrace paddyfield ecosystem structure is"forest-village-terrace paddyfield-river"in the order of descending *** and water samples were sequen-tially taken from forests,villages and the terrace paddy-fields to be able to study the vertical characteristics of Hani’s terrace ***_(4)-P and NH_(3)-N in water were measured to test for water *** soil nutrient factors were tested,including organic material(OM),char and nitrogen ratio(C/N),pH,total nitrogen(TN),total phosphorus(TP),available phosphorus(AP),and available potassium(AK).soil quality was also eval-uated using the characteristics of the soil nutrient *** changes in the landscape,wetland types,wetland plants,hydrology and soil nutrients were *** showed that:(1)Hani’s terrace paddyfield can be divided into five types of wetlands;the rice varieties and cultivation patterns vary in each type of wetland.(2)Hani’s terrace paddyfield has a great capacity for water conservation and a strong ability to purify *** impoundage of Hani’s terrace paddyfield is about 5050 m^(3)/hm^(2).Contaminants in the terrace paddyfield soils decrease exponentially with the decline in altitude.(3)Comparison of soil quality in five different land use types indicates decreasing soil quality from forest to terrace land to terrace paddyfield to water *** for headwater soil,single factors such as OM,TN and TP,and the comp

关键词： Hani’s terrace paddyfield wetland landscape wetland types wetland water wetland soil vertical characteristics

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