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Antioxidative properties of phenolic compounds isolated from the fungal endophytes of Zingiber nimmonii （J. Graham） Dalzell.

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Frontiers in Biology 2017年第2期12卷 151-162页

作者： Madhuchhanda Das Harischandra Sripathy Prakash Monnanda Somaiah Nalini Department of Studies in Botany University of Mysore Manasagangotri Mysore - 570 006 Karnataka India Department of Studies in Bioteehnology University of Mysore Manasagangotri Mysore- 570 006 Karnataka India

BACKGROUND： The microbes living in planta termed ＇endophytes＇ is bestowed with the potential to produce bioactive substances. The aim of this investigation was focused on the isolation and molecular identification of the fungal endophytes from Zingiber nimmonii （J. Graham） Dalzell., an endemic medicinal plant species of the ＇Western ghats＇, a hotspot location in southern India and characterization of the secondary metabolites responsible for the ant^oxidant and DNA protective capacity using chromatography and mass spectrometry techniques. METHODS： Endophytic fungi were isolated and identified by sequencing the Internal Transcribed Spacer （ITS）. The secondary metabolites were extracted with ethyl acetate and evaluated for the total phenolic, flavonoid and antioxidant capacities. The isolates with potential antioxidative property were further analyzed for the DNA protection ability and the presence ofbioactive phenolic compounds by High Performance Liquid Chromatography （HPLC） and Electrospray Ionization- Mass Spectroscopy/Mass Spectroscopy （ESI-MS/MS） techniques. RESULTS： Endophytic fungi belonging to 11 different taxa were identified. The total phenolic content of the extracts ranged from 10.8±0.7 to 81.6±6.0 mg gallic acid equivalent/g dry extract. F lavonoid was present in eight extracts in the range of 5.2 ±0.5 to 24.3±0.9 mg catechin equivalents/g dry extract. Bipolaris specifera, Alternaria tenuissima, Aspergillus terreus, Nectria haematococca and Fusarium chlamydosporum extracts exhibited a potentially high ant^oxidant capacity. Characterization of the extracts revealed an array of phenolic acids and flavonoids. N. haematococca and E chlamydosporum extracts contained quercetin and showed DNA protection ability. CONCLUSION： This study is the first comprehensive report on the fungal endophytes from Z. nimmonii, as potential sources of antioxidative and DNA protective compounds. The study indicates that Z. nimmonii endophytes are potential sources of antioxidants

关键词： endophytic fungi Zingiber westem ghats phenolic acids flavonoid DNA protection

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