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Application of biomonitoring and support vector machine in water quality assessment

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第4期13卷 327-334页

作者： Yue LIAO Jian-yu XU Zhu-wei WANG Institute of Information Science and Technology Ningbo UniversityNingbo 315211China Cisco Systems(China)Research and Development Company Limited Hangzhou 310012China

The behavior of schools of zebrafish （Danio rerio） was studied in acute toxicity environments. Behavioral features were extracted and a method for water quality assessment using support vector machine （SVM） was de- veloped. The behavioral parameters of fish were recorded and analyzed during one hour in an environment of a 24-h half-lethal concentration （LC50） of a pollutant. The data were used to develop a method to evaluate water quality, so as 6＋ 2＋ to give an early indication of toxicity. Four kinds of metal ions （Cu2~, Hg2~, Cr , and Cd ） were used for toxicity testing. To enhance the efficiency and accuracy of assessment, a method combining SVM and a genetic algorithm （GA） was used. The results showed that the average prediction accuracy of the method was over 80% and the time cost was acceptable. The method gave satisfactory results for a variety of metal pollutants, demonstrating that this is an effective approach to the classification of water quality.

关键词： water assessment Behavioral feature parameter Support vector machine (SVM) Genetic algorithm (GA) water quality classification

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assessment of Domestic water Sources Safety: Application of the Micro Biological Survey Method and Microbiological Profile of the Contaminating Bacteria

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Journal of water Resource and Protection 2021年第5期13卷 350-361页

作者： Sali Ben Béchir Adogaye Mabvouna Biguioh Rodrigue Nkamedjie Pete Patrick Martial Andrillene Laure Deutou Wondeu Sanou Sobze Martin Jean Blaise Kemogne Carla Montesano Colizzi Vittorio Department of Biology Faculty of Sciences University of Rome “Tor Vergata” Rome Italy Evangelical University of Cameroon Mbouo-Bandjoun Cameroon Faculty of Medicine and Pharmaceutical Sciences University of Dschang Dschang Cameroon

water is essential for human life and it’s expected to be clean and safe. In Cameroon, government efforts to improve access to drinking water are mitigated. Many households are suffering from chronic water shortages leading to the majority of people using doubtful water sources. This study was carried out in accordance with guidelines produced by the STARBIOS2-EU funded project on Responsible Research and Innovation and aimed to assess the microbiological quality of water samples collected from domestic water sources in 3 divisions of the West region of Cameroon. 22 water samples from well water, stream, water pump and river were aseptically collected. At each sampling point, 50 mL of sample was taken aseptically and immediately transported to the laboratory for analysis. The detection of Coliforms bacteria was done using Micro Biological Survey (MBS) method. 1 mL of each sample was inoculated in the Coliforms MBS (Coli MBS) vial initially rehydrated with 10 mL of sterile distilled water. The Coli MBS vials were closed, shaken for homogenization, and then incubated at 37°C. From the initial red color of the Coli MBS vials, changes in color to yellow of the reaction vials were monitored at three different time intervals (12 h, 19 h and 24 h), corresponding to three levels of bacterial concentration. All positives samples on MBS method were selected to determine the presence of E. coli Loop full broths of Coli MBS vials were taken;streaked into the eosin methylene blue (EMB) agar plate and incubated at 37°C for 24 hours. E. coli was confirmed by observing green metallic sheen on EMB agar plate. The biochemical indole, methyl red, Voges-Proskauer and citrate (IMViC) test was performed to determine the complete microbiological profile of the water samples. Almost all the water samples were contaminated with Total Coliforms (TC). A high concentration of TC (>103 CFU/mL) was found in 8 samples (36.4

关键词： water assessment Micro Biological Survey Method Biochemical IMViC Test West Cameroon

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