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Reuse of waste frying oil for production of rhamnolipids using Pseudomonas aeruginosa zju.u1M

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第9期8卷 1514-1520页

作者： ZHU Yong GAN Jun-jiang ZHANG Guo-liang YAO Bin ZHU Wen-jie MENG Qin Shool of Materials Science and Chemical Engineering Zhejiang University Hangzhou 310027 China School of Biological and Chemical Engineering Zhejiang Uiversity of Science and Technology Hangzhou 310032 China Institute of Biological and Environmental Engineering Zhejiang University of Technology Hangzhou 310014 China

In this work,rhamnolipid production was investigated using waste frying oil as the sole carbon source. By culture in shaking flasks,a naturally isolated strain synthesized rhamnolipid at concentration of 12.47 g/L and its mutant after treatment by UV light increased this productivity to 24.61 g/L. Fermentation was also conducted in a 50 L bioreactor and the productivity reached over 20 g/L. Hence,with a stable and high productive mutant strain,it could be feasible to reuse waste frying oil for rhamnolipid production on industrial scale.

关键词： Rhamnolipid Pseudomonas aeruginosa Biosurfactant waste frying oil

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Optimizing rhamnolipid production by Pseudomonas aeruginosa ATCC 9027 grown on waste frying oil using response surface method and batch-fed fermentation

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Journal of Central South University 2013年第4期20卷 1015-1021页

作者：罗致袁兴中钟华曾光明刘智峰马小玲朱雅婷 College of Environmental Science and Engineering Hunan University Key Laboratory of Environmental Biology and Pollution Control of Ministry of Education (Hunan University)

Rhamnolipid production by Pseudomonas aeruginosa ATCC 9027 with waste frying oil as sole carbon source was studied using response surface method. Cultures were incubated in shaking flask with temperature, NO3- and Mg2+ concentrations as the variables. Meanwhile, fed-batch fermentation experiments were conducted. The results show that the three variables are closely related to rhamnolipid production. The optimal cultivation conditions are of 6.4 g/L NaNO3 , 3.1 g/L MgSO4 at 32 ℃, with the maximum rhamnolipid production of 6.6 g/L. The results of fed-batch fermentation experiments show that feeding the oil in two batches can enhance rhamnolipid production. The best time interval is 72 h with the maximum rhamnolipid production of 8.5 g/L. The data are potentially useful for mass production of rhamnolipid on oil waste with this bacterium.

关键词： Pseudomonas aeruginosa rhamnolipid waste frying oil response surface method fed-batch

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Combined Effect of a Catalytic Reduction Device with waste frying oil-Based Biodiesel on NOx Emissions of Diesel Engines

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Modern Mechanical Engineering 2022年第3期12卷 63-73页

作者： Samson K. Fasogbon Vincent N. Ugwah Olaleye M. Amoo Patrick Ajaero Ogagaoghene D. Emma-Egoro Department of Mechanical Engineering University of Ibadan Ibadan Nigeria Centre for Petroleum Energy Economics and Law University of Ibadan Ibadan Nigeria Department of Agricultural and Bioresource Engineering Federal University of Technology Owerri Imo State Nigeria Department of Systems Engineering Stevens Institute of Technology Hoboken USA Department of Chemical Engineering Landmark University Kwara State Nigeria

Internal combustion engines with application in automobiles and other relevant industries constitute significant environmental pollution via the release of toxic exhaust gasses like carbon monoxide (CO), hydrocarbons (HC), particulate matter (PM), and nitrogen oxide (NOx). Engine researchers and manufacturers are challenged to develop external and internal measures to ensure environmentally friendly solutions to accommodate and conform to the growing list of emission standards. Therefore, this work presents an experimental investigation of the NOx emission profile of a diesel engine that is fuelled and fitted with waste frying oil-based biodiesel and catalytic converter. Using a single-cylinder, four-stroke air-cooled CI engine at a constant speed of 1900 rpm and different loadings of 25%, 50%, 75%, and 100%;fitted with a catalytic converter at the exhaust outlet of the engine and linked to a dynamometer and a gas analyser, an experiment was conducted at biodiesel/diesel volume blends of B0 (0/10), B5 (5/95), B20 (20/80), B30 (30/70), B70 (70/30), B100 (100/0);and 30% concentration (v/v), 0.5 litre/hr flow rate of aqueous urea from the catalytic converter. The results show an increasing NOx emission as the biodiesel component increased in the blend. The catalytic converter showed a downward NOx reduction with a significant 68% reduction in efficiency at high exhaust gas temperatures. It is concluded that the combined utilisation of waste frying oil-based biodiesel and the catalytic converter yields substantial NOx emission reduction.

关键词： Catalytic Converter waste frying oil Biodiesel NOx Emission Diesel Engines

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