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wrky transcription factors down-regulate the expression of H2S-generating genes, LCD and DES in Arabidopsis thaliana

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Science Bulletin 2015年第11期60卷 995-1001,I0007页

作者：刘志强方慧慧裴雁曦金竹萍张丽萍刘旦梅 School of Life Science Shanxi University

Hydrogen sulfide(H2S) is a gasotransmitter playing a vital role in response to biotic and abiotic stress for plants. In order to understand the transcriptional regulation of the genes that are responsible for endogenous H2 S generation, the promoter sequences of L-cysteine desulfhydrase(LCD), D-cysteine desulfhydrase(DCD1,DCD2), desulfhydrase(DES) and nitrogen fixation synthetase(NFS1, NFS2) were analyzed. They are all found to contain a W-box, a characteristic core binding site for the plant wrky transcription factors, which have important roles in the plant's responses to numerous stresses by modifying the expression patterns of their target genes. An electrophoretic mobility shift assay indicated that wrky18 and wrky60 interacted with the W-box in the promoters of the LCD, DCD1, DCD2, DES and NFS2 genes, whereas wrky40 bound to the W-box of the NFS1 promoter. The expression levels of the LCD, DES and DCD1 genes were up-regulated, but the DCD2 was downregulated in the plants with wrky18, wrky40 or wrky60 mutations. The plants with double and triple mutations of wrky18, wrky40 and wrky60 had a higher rate of H2 S production during cadmium stress and were more resistant to the cadmium stress than the wild type or single mutants. These results suggest that wrky transcription factors regulate the H2 S signaling pathway in plants, allowing them to cope with cadmium stress.

关键词： Hydrogen sulfide wrky transcription factors Cadmium stress Arabidopsis

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Protein-Protein Interactions in the Regulation of wrky transcription factors

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Molecular Plant 2013年第2期6卷 287-300页

作者： Yingjun Chi Yan Yang Yuan Zhou Jie Zhou Baofang Fan Jing-Quan Yu Zhixiang Chen Department of Horticulture Zhejiang University Hangzhou China Department of Botany and Plant Pathology Purdue University West Lafayette IN 47907 USA

It has been almost 20 years since the first report of a wrky transcription factor, SPF1, from sweet potato. Great progress has been made since then in establishing the diverse biological roles of wrky transcription factors in plant growth, development, and responses to biotic and abiotic stress. Despite the functional diversity, almost all ana-lyzed wrky proteins recognize the TrGACC/T W-box sequences and, therefore, mechanisms other than mere recognition of the core W-box promoter elements are necessary to achieve the regulatory specificity of wrky transcription factors. Research over the past several years has revealed that wrky transcription factors physically interact with a wide range of proteins with roles in signaling, transcription, and chromatin remodeling. Studies of wrky-interacting proteins have provided important insights into the regulation and mode of action of members of the important family of transcrip-tion factors. It has also emerged that the slightly varied wrky domains and other protein motifs conserved within each of the seven wrky subfamilies participate in protein-protein interactions and mediate complex functional interactions between wrky proteins and between wrky and other regulatory proteins in the modulation of important biologi- cal processes. In this review, we summarize studies of protein-protein interactions for wrky transcription factors and discuss how the interacting partners contribute, at different levels, to the establishment of the complex regulatory and functional network of wrky transcription factors.

关键词： wrky transcription factors protein-protein interactions VQ proteins protein phosphorylation chromatinremodeling HDA19,

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Sugarcane transcription factor Scwrky4 negatively regulates resistance to pathogen infection through the JA signaling pathway

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The Crop Journal 2024年第1期12卷 164-176页

作者： Dongjiao Wang Wei Wang Shoujian Zang Liqian Qin Yanlan Liang Peixia Lin Yachun Su Youxiong Que Key Laboratory of Sugarcane Biology and Genetic Breeding Ministry of Agriculture and Rural AffairsKey Laboratory of GeneticsBreeding and Multiple Utilization of CropsMinistry of EducationCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina National Key Laboratory for Tropical Crop Breeding Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesSanya 572024HainanChina

wrky transcription factors,transcriptional regulators unique to plants,play an important role in defense response to pathogen ***,the resistance mechanisms of wrky genes in sugarcane remain *** the present study,gene ontology(GO)enrichment analysis revealed that wrky gene family in sugarcane was extensively involved in the response to biotic stress and in defense *** identified gene Scwrky4,a classⅡc member of the wrky gene family,in sugarcane cultivar *** gene was induced by salicylic acid(SA)and methyl jasmonate(MeJA)***,expression of Scwrky4 was down-regulated in smut-resistant sugarcane cultivars but up-regulated in smutsusceptible sugarcane cultivars infected with Sporisorium ***,stable overexpression of the Scwrky4 gene in Nicotiana benthamiana enhanced susceptibility to Fusarium solani *** and caused down-regulated expression of immune marker-related *** analysis indicated suppressed expression of most JAZ genes in the signal transduction ***4 interacted with ScJAZ13 to repress its *** thus hypothesized that the Scwrky4 gene was involved in the regulatory network of plant disease resistance,most likely through the JA signaling *** present study depicting the molecular involvement of Scwrky4 in sugarcane disease resistance lays a foundation for future investigation.

关键词： Disease resistance Expression profile Transcriptome analysis wrky transcription factors

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Novel wrky transcriptional factors associated with NPR1 during systemic acquired resistance in barley are potential resources to improve wheat resistance to Puccinia triticina

Novel WRKY transcriptional factors associated with NPR1 duri...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Li Huanpeng Wu Jiaojiao Gao Jing Bi Weishuai Yu Xiumei Liu Daqun Wang Xiaodong College of Plant Protection Biological Control Center for Plant Diseases and Plant Pests of HebeiHebei Agricultural University College of Life Science Hebei Agricultural University Graduate School of Chinese Academy of Agricultural Sciences

In Arabidopsis,both initial pathogen infection and benzothiadiazole(BTH) treatment are sufficient to induce systemic acquired resistance(SAR),which is an inducible form of enhanced resistance to secondary pathogen ***1 is reported to be the master regulator of *** Arabidopsis and rice,wrky transcription factors are designated as key components during SAR in either NPR1-dependent or-independent *** several SAR-like responses discovered in wheat and barley can improve their resistance to various fungal diseases including Blumeria graminis and Puccinia triticina(Pt),roles of NPR1 and wrky genes during SAR in these crop species are largely *** a series of transcriptome analysis on barley transgenic lines over-expressing wheat wNPR1(wNPR1-OE) and knocking down barley HvNPR1(HvNPR1-Kd),the regulatory networks of NPR1 during SAR triggered by Pseudomonas syringae *** DC3000 and BTH were clarified.A total of 17 differentially expressed wrky genes were selected for further functional ***,some of them may also function through physical interaction with NPR1 *** transient expression of three novel wrky genes,including Hvwrky165,Hvwrky213,and Hvwrky248,in wheat leaves by Agrobacterium-mediated infiltration enhanced the resistance to *** transgenic lines over-expressing these wrky genes may be potential resources to improve wheat resistance to various fungal diseases.

关键词： wrky transcription factors NPR1 Systemic acquired resistance Barley Wheat Puccinia triticina

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Differential expression of rice VQ protein gene family in response to nitric oxide and the regulatory circuit of VQ7 and wrky24

Differential expression of rice VQ protein gene family in re...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： Wang Haihua Xiao Ting Meng Jiao Tao Zong Peng Xixu Zhou Dinggang Tang Xinke School of Life Science Hunan University of Science and Technology

The functional diversity of plant-specific VQ proteins are closely associated with their partners wrky transcription factors,and with a complex network of signaling pathways that mediated by hormone ***,no information is available about the expression patterns of VQ genes modulated by nitric oxide(NO),thus posing a hindrance to defining the mechanisms underlying the involvement of VQ proteins in NO signaling ***,we report genome-wide expression profiles of the differentially expressed rice VQ genes under NO treatment based on a microarray *** analysis of expression patterns revealed that some VQ genes and wrky genes share similar expression *** of cis-elements shows that W-box,or W-box like sequences are overrepresented within the promoters of most of the NO-responsive VQ *** particular,the similarly expressed VQ1 and wrky24 showed great induction upon NO *** expression assay demonstrated that wrky24 specifically bound to the promoter regions of VQ1 and wrky2A itself,which contain a multiple of copies of W-box or W-box like ***-two-hybrid assay indicated wrky24 could interact physically with VQ7 through the C-terminal wrky ***,VQ7 and wrky24 formed an autoand cross-regulation circuit that is required for tight regulation and fine-tuning of physiological processes they are involved *** findings provide a solid foundation for exploring the specific functions of VQ protein family in NO signaling pathway.

关键词： VQ proteins Expression profiles wrky transcription factors Nitric oxide Regulatory circuit Rice

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