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Effects of Acupotomy on Immobilization-Induced Gastrocnemius Contracture and Fibrosis in Rats via wnt/β-catenin signaling

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Chinese Journal of Integrative Medicine 2024年第7期30卷 633-642页

作者： HU Rui PAN Jun-kang LI Jia-hui ZHANG Han LI Shao-rong ZHANG Yi School of Acupuncture-Moxibustion and Tuina Beijing University of Chinese MedicineBeijing100029China Acupuncture Department Kunming Municipal Hospital of Traditional Chinese MedicineKunming650500China

Objective To determine whether acupotomy ameliorates immobilization-induced muscle contracture and fibrosis via wnt/β-catenin signaling *** Thirty Wistar rats were randomly divided into 5 groups(n=6)by a random number table,including control,immobilization,passive stretching,acupotomy,and acupotomy 3 weeks(3-w)*** rat model of gastrocnemius contracture was established by immobilizing the right hind limb in plantar flexion for 4 *** in the passive stretching group received passive stretching at gastrocnemius,a daily series of 10 repetitions for 30 s each at 30-s intervals for 10 consecutive *** in the acupotomy and acupotomy 3-w groups received acupotomy once and combined with passive stretching at gastrocnemius a daily series of 10 repetitions for 30 s each at 30-s intervals for 10 consecutive ***,rats in the acupotomy 3-w group were allowed to walk freely for 3 weeks after 10-day *** treatment,range of motion(ROM),gait analysis[i.e.,paw area,stance/swing and maximum ratio of paw area to paw area duration(Max dA/dT)],gastrocnemius wet weight and the ratio of muscle wet weight to body weight(MWW/BW)were *** morphometric and muscle fiber cross-sectional area(CSA)were assessed by hematoxylin-eosin ***-related mRNA expressions(i.e.,wnt 1,β-catenin,axin-2,α-smooth muscle actin,fibronectin,and types I and III collagen)were measured using real-time quantitative polymerase chain *** 1,β-catenin and fibronectin concentrations were measured by enzyme-linked immunosorbent *** I and III collagen in the perimysium and endomysium were analyzed using *** Compared with the control group,ROM,gait function,muscle weight,MWW/BW and CSA were significantly decreased in the immobilization group(all Pwnt 1,β-catenin,fibronectin and mRNA levels of fibrosis-related genes were obviously increased(all P<0.01).Tr

关键词： acupotomy immobilization muscle contracture muscle fibrosis wnt/β-catenin signaling

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scRNA-seq reveals that origin recognition complex subunit 6 regulates mouse spermatogonial cell proliferation and apoptosis via activation of wnt/β-catenin signaling

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Asian Journal of Andrology 2024年第1期26卷 46-56页

作者： Shi-Wei Liu Jia-Qiang Luo Liang-Yu Zhao Ning-Jing Ou Chao-Yang Yu-Xiang Zhang Hao-Wei Bai Hong-Fang Sun Jian-Xiong Zhang Chen-Cheng Yao Peng Li Ru-Hui Tian Zheng Li Zi-Jue Zhu Department of Andrology Center for Men's HealthUrologic Medical CenterShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China State Key Lab of Reproductive Medicine Nanjing Medical UniversityNanjing 211166China Shanghai Key Lab of Reproductive Medicine Shanghai Jiao Tong University School of MedicineShanghai 200080China Department of Urology Guangdong Provincial Key Laboratory of Biomedical ImagingThe Fifth Affiliated HospitalSun Yat-sen UniversityZhuhai 519000China Department of Urology First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China

The regulation of spermatogonial proliferation and apoptosis is of great significance for maintaining *** single-cell RNA sequencing(scRNA-seq)analysis of the testis was performed to identify genes upregulated in *** scRNAseq analysis,we identified the spermatogonia upregulated gene origin recognition complex subunit 6(Orc6),which is involved in DNA replication and cell cycle regulation;its protein expression in the human and mouse testis was detected by western blot and *** explore the potential function of Orc6 in spermatogonia,the C18-4 cell line was transfected with control or Orc6 ***,5-ethynyl-2-deoxyuridine(EdU)and terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)assays,flow cytometry,and western blot were used to evaluate its effects on proliferation and *** was revealed that ORC6 could promote proliferation and inhibit apoptosis of C18-4 *** RNA sequencing and bioinformatics analysis indicated that Orc6 was involved in the activation of wingless/integrated(wnt)/β-catenin *** blot revealed that the expression ofβ-catenin protein and its phosphorylation(Ser675)were significantly decreased when silencing the expression of *** findings indicated that Orc6 was upregulated in spermatogonia,whereby it regulated proliferation and apoptosis by activating wnt/β-catenin signaling.

关键词： cell proliferation ORCG scRNA-seq analysis spermatogonia wnt/β-catenin signaling

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Xiao Tan San Jie Fang Hampers the Growth of Colon Cancer Stem Cells through the wnt/β-catenin signaling Pathway

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World Journal of Traditional Chinese Medicine 2024年第2期10卷 171-179页

作者： Yu-Qi Zhou Jun-Fei Yuan Hong-Shi Shen Ya-Ping Wang Han-Fei Shi De-Jian Pan Min Ye Department of Hematology and Oncology The 904^(th) Hospital of PLA Joint Logistic Support ForceWuxiJiangsuChina Department of Clinical Laboratory The 904^(th)Hospital of PLA Joint Logistic Support ForceWuxiJiangsuChina Department of Traditional Chinese Medicine Second Affiliated Hospital of Naval Medical UniversityShanghaiChina

Objective: The purpose of this research was to examine the potential anticancer properties of Xiao Tan San Jie Fang(XTSJF) and its potential mechanism of action against colorectal cancer. Materials and Methods: HCT116 cells were induced into HCT116 spheres in DMEM/F12 medium by treatment with epidermal growth factor + fibroblast growth factor + leukemia inhibitory factor + B27. The proliferation ability and stemness of HCT116 spheres was examined. Various concentrations of XTSJF were used to treat HCT116 spheres to observe the impact on proliferation, apoptosis, and expression of stem cell markers. Next, wnt/β-catenin pathway-related factor proteins were detected. Results: The findings revealed that XTSJF suppressed cell growth and induced cell death in HCT116 cells in a dosage-dependent manner. Similarly, XTSJF promotes apoptosis, inhibits cell proliferation, prolongs survival, and maintains the expression of stem cells through the wnt/-catenin/TCF4 axis. XTSJF also inhibits AKT activity and subsequently activates glycogen synthesis kinase-3β expression, inhibiting wnt/beta-catenin pathway activity and downstream target gene transcript expression. The wnt/β-catenin signaling pathway is inhibited by the XTSJF, leading to the suppression of colon cancer stem cell proliferation. Conclusion: Xiaotan Sanjie Prescription inhibited colon cancer stem cell growth through wnt/β-catenin signaling pathway.

关键词： Apoptosis colorectal cancer proliferation traditional Chinese medicine wnt/β-catenin signaling

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wnt/β-catenin signaling pathway is active in pancreatic development of rat embryo

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World Journal of Gastroenterology 2006年第16期12卷 2615-2619页

作者： Qi-Ming Wang Ye Zhang Kai-Ming Yang Hong-Ying Zhou Hui-Jun Yang Department of Anatomy School of Preclinical and Forensic Medicine Sichuan University Chengdu 610041 China Beida Weixin Biology and Science Company Beijing 100081 China

AIM： To elucidate the role of wnt/β-catenin signaling pathway in pancreatic development of rat embryo. METHODS： The mRNAs of β-catenin, APC, cyclin D1 genes were amplified by means of semiquantitative reverse transcription polymerase chain reaction （RTPCR） from embryonic pancreas in different periods and normal pancreas of rat, respectively. Protein expression of these genes in embryonic pancreas of E14.5-E18.5 was examined by immunohistochemical method. RESULTS： In embryonic pancreas of E14.5, the transcript amplification of β-catenin and cyclinD1 genes was detected. In embryonic pancreas of E18.5, the transcription levels of β-catenin and cyclinD1 genes became much higher than in other periods. But in adult rat pancreas the transcription of cyclinD1 gene could not be observed. Only until E18.5, the transcript amplification of mRNA of APC gene could be detected. Surprisingly, the transcription level of APC gene became much higher in adult rat pancreas than in embryonic pancreas. By means of immunohistochemical staining, identical results were obtained to the above by RP-PCR, except for β-catenin protein in adult rat pancreas. CONCLUSION： Active wnt/β-catenin signaling occurs in rat embryonic pancreas and is probably important for pancreatic development and organ formation.

关键词： wnt/β-catenin signaling Pancreas Development Embryo

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The wnt/β-catenin signaling pathway in the tumor microenvironment of hepatocellular carcinoma

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Cancer Biology & Medicine 2022年第3期19卷 305-318页

作者： Kaiting Wang Xinyao Qiu Yan Zhao Hongyang Wang Lei Chen School of Life Sciences Fudan UniversityShanghai 200438China Fudan University Shanghai Cancer Center Department of OncologyShanghai Medical CollegeFudan UniversityShanghai 200032China Institute of Metabolism&Integrative Biology(IMIB) Fudan UniversityShanghai 200438China The International Cooperation Laboratory on Signal Transduction Eastern Hepatobiliary Surgery HospitalSecond Military Medical UniversityShanghai 200438China

The wnt/β-catenin signaling pathway regulates many aspects of tumor biology,and many studies have focused on the role of this signaling pathway in tumor ***,it is now clear that tumor development and metastasis depend on the two-way interaction between cancer cells and their environment,thereby forming a tumor microenvironment(TME).In this review,we discuss how wnt/β-catenin signaling regulates cross-interactions among different components of the TME,including immune cells,stem cells,tumor vasculature,and noncellular components of the TME in hepatocellular *** also investigate their preclinical and clinical insights for primary liver cancer intervention,and explore the significance of using wnt/β-catenin mutations as a biomarker to predict resistance in immunotherapy.

关键词： wnt/β-catenin signaling HCC tumor microenvironment immunotherapy

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CDK11 negatively regulates wnt/β-catenin signaling in the endosomal compartment by affecting microtubule stability

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Cancer Biology & Medicine 2020年第2期17卷 328-342页

作者： Danmin Ou Lin Chen Jiang He Zhuoxian Rong Jie Gao Liyu Liu Feiyu Tang Jiang Li Yuezhen Deng Lunquan Sun Department of Oncology Center for Molecular MedicineXiangya HospitalCentral South UniversityChangsha 410008China international Cooperation Base of Cancer Precision Therapy Department of Science and Technology of Hunan ProvinceChangsha 410008China Key Laboratory of Molecular Radiation Oncology of Hunan Province Changsha 410008China National Clinical Research Center for Geriatric Disorders Changsha 410008China

Objectives:Improper activation of wnt/β-catenin signaling has been implicated in human *** the well-studied glycogen synthase kinase 3p(GSK3p)and casein kinase 1(CK1),other kinases affecting wnt/β-catenin signaling remain to be ***:To identify the kinases that modulate wnt/β-catenin signaling,we applied a kinase small interfering RNA(siRNA)library screen *** assays,immunoblotting,and real-time polymerase chain reaction(PCR)were performed to confirm the regulation o f the wnt/β-catenin signaling pathway by cyclin-dependent kinase 11(CDK11)and to investigate the underlying *** immunofluorescence,coimmunoprecipitation(co-IP),and scratch wound assays were used to demonstrate colocalization,detect protein interactions,and explore the function of ***:CDK11 was found to be a significant candidate kinase participating in the negative control of wnt/P-catenin ***-regulation of CDK11 led to the accumulation of wnt/β-catenin signaling receptor complexes,in a manner dependent on intact adenomatosis polyposis coli(APC)*** analysis showed that CDK11 modulation of wnt/P-catenin signaling engaged the endolysosomal machinery,and CDK11 knockdown enhanced the colocalization of wnt/β-catenin signaling receptor complexes with early endosomes and decreased colocalization with ***,CDK11 was found to function in wnt/β-catenin signaling by regulating microtubule *** of CDK11 down-regulated ***,co-IP assays demonstrated that CDK11 interacts with the a-tubulin deacetylase SIRT2,whereas SIRT2 down-regulation in CDK11-depleted cells reversed the accumulation of wnt/(3-catenin signaling receptor ***11 was found to suppress cell migration through altered W nt/β-catenin ***:CDK11 is a negative modulator of wnt/β-catenin signaling that stabilizes microtubules,thus resulting in the dysregulation of receptor complex trafficking

关键词： wnt/β-catenin signaling CDK11 endosome microtubule SIRT2

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Magnesium implantation or supplementation ameliorates bone disorder in CFTR-mutant mice through an ATF4-dependent wnt/β-catenin signaling

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Bioactive Materials 2022年第2期7卷 95-108页

作者： Jiankun Xu Peijie Hu Xiaotian Zhang Junjiang Chen Jiali Wang Jieting Zhang Ziyi Chen Mei Kuen Yu Yiu Wa Chung Yan Wang Xiaohu Zhang Yifeng Zhang Nianye Zheng Hao Yao Jiang Yue Hsiao Chang Chan Ling Qin Ye Chun Ruan Musculoskeletal Research Laboratory Department of Orthopedics&TraumatologyThe Chinese University of Hong KongHong KongChina Deparment of Biomedical Engineering Faculty of EngineeringThe Hong Kong Polytechnic UniversityHong KongChina Epithelial Cell Biology Research Centre School of Biomedical SciencesFaculty of MedicineThe Chinese University of Hong KongHong KongChina School of Biomedical Engineering Sun Yat-sen UniversityGuangzhouChina School of Life Science and Technology ShanghaiTech UniversityShanghaiChina

Magnesium metal and its alloys are being developed as effective orthopedic implants;however,the mechanisms underlying the actions of magnesium on bones remain *** fibrosis,the most common genetic disease in Caucasians caused by the mutation of CFTR,has shown bone disorder as a key clinical manifestation,which currently lacks effective therapeutic *** we report that implantation of magnesium-containing implant stimulates bone formation and improves bone fracture healing in CFTR-mutant ***/β-catenin signaling in the bone is enhanced by the magnesium implant,and inhibition of wnt/β-catenin by iCRT14 blocks the magnesium implant to improve fracture healing in CFTR-mutant *** further demonstrate that magnesium ion enters osteocytes,increases intracellular cAMP level and activates ATF4,a key transcription factor known to regulate wnt/β-catenin *** vivo knockdown of ATF4 abolishes the magnesium implant-activated β-catenin in bones and reverses the improved-fracture healing in CFTR-mutant *** addition,oral supplementation of magnesium activates ATF4 and β-catenin as well as enhances bone volume and density in CFTR-mutant ***,these results show that magnesium implantation or supplementation may serve as a potential anabolic therapy for cystic fibrosis-related bone *** of ATF4-dependent wnt/β-catenin signaling in osteocytes is identified as a previously undefined mechanism underlying the beneficial effect of magnesium on bone formation.

关键词： Magnesium implant Cystic fibrosis-related bone disorder ATF4 wnt/β-catenin signaling

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Involvement of wnt/β-catenin signaling-mediated hippocampal neurogenesis in chronic pain associated anxiety

Involvement of Wnt/β-catenin signaling-mediated hippocampal...

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中国神经科学学会第十二届全国学术会议

作者： You-Yi ZHAO Li ZHANG Ji-Ping YANG Xiu-Fen ZHANG Xin-Yu LIU Ya-Hui SHEN Ya-Zhou WANG Sheng-Xi WU Department of Neurobiology and Collaborative Innovation Center for Brain Science School of Basic Medicine Fourth Military Medical University Chengdu Medical College Institute of Basic Medical Science Department of Human Anatomy Xi'an Medical University

Objective Chronic pain is usually accompanied by anxiety. Previous studies have revealed that hippocampal neuronal activity is involved in stress and chronic anxiety. As is known, neurogenesis persists in adult hippocampus and has been suggested to be involved in chronic depression. However, it has been poorly investigated whether and how hippocampal neurogenesis is involved in the chronic-pain associated anxiety. wnt/β-catenin signaling is an important signaling pathway which plays essential roles in the hippocampal neurogenesis. The present study aims to investigate whether the wnt/β-catenin signaling-mediated hippocampal neurogenesis is actively involved in the chronic-pain associated anxiety. Methods Spare-nerve injury was performed to model chronic pain. Anxiety behavior was evaluated at 21 dpi by elevated plus maze and open field assay. Brd U was administered for 14 successive days after injury and mice were sacrificed at 21 dpi. A widely adopted wnt/β-catenin signaling reporter mouse line Topgal mice were used to read the change of wnt signaling. NestinCre ER: β-cateninloxp/loxp mice（β-catenin CKO） were used to deplete β-catenin in hippocampal neural stem cells. Nestin-Cre ER: β-catenin EX3 loxp/loxp mice（β-catenin EX3） were used to express stabilized β-catenin in hippocampal neural stem cells. Tamoxifen was administered to activate Nestin-Cre. In most experiments, mice were sacrificed after behavior tests. Immunohistochemistry and Western-blotting were performed to assess neurogenesis and the activation of wnt/β-catenin signaling. Results（1） Significant decrease of pain threshold was observed in all the mice with SNI from 1 to at least 28 days post injury. At 21 dpi, open field assay and elevated plus maze showed that most of the SNI mice exhibited obvious anxiety behavior.（2） As compared with sham control, SNI mice showed significant decrease of hippocampal neurogenesis.（3） wnt/β-catenin signaling in hippocampus was reduced in SNI mice.（4） β-catenin CKO mic

关键词： chronic pain anxiety neurogenesis wnt/β-catenin signaling

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A Caspase-Dependent Pathway Is Involved in wnt/β-catenin signaling Promoted Apoptosis in Bacillus Calmette-Guerin Infected RAW264.7 Macrophages

A Caspase-Dependent Pathway Is Involved in Wnt/β-Catenin Si...

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第四届全国人畜共患病学术研讨会 2014年中国狂犬病年会中国畜牧兽医学会兽医公共卫生学分会第四次学术研讨会

作者： Xiaoling Wu Guangcun Deng Xiujing Hao Yong Li Jin Zeng Chunyan Ma Yulong He Xiaoming Liu Yujiong Wang Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China College of Life Science Ningxia University

Apoptosis of alveolar macrophages following Mycobacterium tuberculosis infection have been demonstrated to play a central role in the pathogenesis of tuberculosis. In the present study, we found that wnt/β-catenin signaling possesses the potential to promote macrophage apoptosis in response to mycobacterial infection. In agreement with other findings, an activation wnt/β-catenin signaling was observed in murine macrophage RAW264.7 cells upon Mycobacterium bovisBacillus Calmette-Guerin(BCG) infection at a multiple-of-infection of 10, which was accompanied with up-regulation of pro-inflammatory cytokines TNF-α and IL-6 production. However, the BCG-induced TNF-α and IL-6 secretion could be significantly reduced when the cells were exposed to a canonical wnt signaling ligand, wnt3 a. Importantly, the activation of wnt/β-catenin signaling was able to further promote apoptosis in BCG-infected RAW264.7 cells in part by a mitochondria-dependent apoptosis pathway. Immunoblotting analysis further demonstrated that wnt/β-catenin signaling-induced cell apoptosis partly through a caspase-dependent apoptosis mechanism by down-regulation of anti-apoptotic protein Mcl-1, and up-regulation of pro-apoptotic proteins Bax and cleaved-caspase-3, as well as enhancement of caspase-3 activity in BCG-infected RAW264.7 cells. These data may imply an underlying mechanism of alveolar macrophages in response to mycobacterial infection, by which the pathogen induces wnt/β-catenin signaling activation, which in turn represses mycobacterium-trigged inflammatory responses and promotes mycobacteria-infected cell apoptosis.

关键词： wnt/β-catenin signaling alveolar macrophages apoptosis caspase mycobacterial infection

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LncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating wnt/β-catenin signaling

LncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis an...

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中国生物化学与分子生物学会2020全国学术在线会议

作者： Jinchang Pan Zhaohui Gong Department of Biochemistry and Molecular Biology Medical School of Ningbo University

MicroRNAs(miRNAs) and Twist 1-induced epithelial-mesenchymal transition(EMT) in cancer cell dissemination are well established,but the involvement of long noncoding RNAs(lncRNAs) in Twist1-mediated signaling remains largely *** the present study,we observed that lncRNA JPX was upregulated in lung cancer metastatic tissues and was closely correlated with tumor size and an advanced ***,JPX promoted lung cancer cell proliferation in vitro and facilitated lung tumor growth in ***,JPX upregulated Twist1 by competitively sponging miR-33 a-5 p and subsequently induced EMT and lung cancer cell ***,JPX and Twist1 were coordinately upregulated in lung cancer tissues and ***,the JPX/miR-33 a-5 p/Twist1 axis participated in EMT progression by activating wnt/β-catenin *** findings suggest that lncRNA JPX,a mediator of Twistl signaling,could predispose lung cancer cells to metastasis and may serve as a potential target for targeted therapy.

关键词： Epithelial-mesenchymal transition Twist1 Long noncoding RNA wnt/β-catenin signaling Lung cancer

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