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HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium

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World Journal of Gastroenterology 2022年第29期28卷 3886-3902页

作者： Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama Laboratorio de Investigación en Enfermedades Infecciosas Hospital Infantil de México Federico GómezMexico City 06720Mexico Posgrado en Biomedicina y Biotecnología Molecular Laboratorio de Producción y Control de BiológicosEscuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalMexico City 11340Mexico Laboratorio de Adquisición de Hierro Universidad Autónoma de la Ciudad MéxicoPosgrado Ciencias GenómicaMexico City 03100Mexico Laboratorio Central de Microscopía Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalMexico City 11340Mexico Centro de estudios económicos y sociales en salud Hospital Infantil de México Federico GómezMexico City 06720Mexico Laboratorio de Investigación en Biología del Desarrollo y Teratogénesis Experimental Hospital Infantil de México Federico GómezMexico City 06720Mexico

BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence *** To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) *** Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional *** HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium *** technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS *** analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein

关键词： Hypothetical protein HP0953 Adherence Helicobacter pylori Glycocalyx virulence factor Persistence

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VmNLP2 from Valsa mali acts as a phytotoxic virulence factor

VmNLP2 from Valsa mali acts as a phytotoxic virulence factor

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中国植物病理学会2021年学术年会

作者： LIU Jianying NIE Jiajun CHANG Yali HUANG Lili State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F University

Necrosis and ethylene-inducing peptide 1(Nepl)-like proteins(NLPs) are typically phytotoxic molecules and play important roles in plant-microbe ***,their biological roles in the pathogenic fungus Valsa mali,the causal agent of apple Valsa canker,remains ***,we identified two NLPs,VmNLPl and VmNLP2 from *** agrobacteriamediated transient expression in Nicotiana benthamiana,only VmNLP2 was shown to induce cell death and was subjected to further *** infiltration of VmNLP2 recombinant protein in apple leaves,we found that VmNLP2 exhibited phytotoxic activity in apple leaves as *** knockout and pathogenicity test showed that VmNLP2-deficient *** strain displayed reduced aggressiveness on *** sequence alignment revealed that the second Histidine(H) among the conserved heptapeptide(GHRHDWE) of VmNLP2 is mutated to Tyrosine(Y).When this Tyrosine(Y) was substituted by Histidine(H),the variant(VmNLP2) showed a stronger cell death 一 inducing activity in ***,complementation of VmNLP2 to VmNLP2 deletion mutant caused larger disease lesion on apple leaves,compared to the *** wild type *** together,we demonstrate that VmNLP2 from *** is an essential virulence factor,and the virulence of VmNLP2 probably correlates to its phytotoxic activity.

关键词： Valsa mali Nep1-like proteins cytotoxicity virulence factor biological roles

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virulence factors in Methicillin-Resistant Staphylococcus aureus Isolated from ICU Units in Brazil

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Advances in Microbiology 2014年第4期4卷 207-215页

作者： Simone G.Souza Guilherme B.Campos Pollianna S.Oliveira Daniel S.Sousa Danilo C.C.Da Silva Verena M.Santos Aline T.Amorim Angelita M.O.G.Santos Jorge Timenetsky Mariluze P.Cruz Regiane Yatsuda Lucas M.Marques Instituto Multidisciplinar em Saúde Núcleo de Tecnologia em SaúdeUniversidade Federal da BahiaVitória da ConquistaBrazil Instituto de Ciências Biomédicas Departamento de MicrobiologiaUniversidade de Sao PauloSao PauloBrazil

Species of Staphylococcus are common in hospital infection (HI). Methicillin resistant S. aureus (MRSA) has also become a serious problem in Brazilian HI. The aim of this study was to characterize the pathogenicity of methicillin-resistant S. aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) isolated in public hospitals. The clinical isolates were obtained from intensive care unit. The MRSA and MSSA strains were genotyped by PCR for detection genes related to virulence factors. Moreover, the strains were tested for biofilm formation and cytokine induction in macrophages. Three strains of MRSA (9.68%) expressed the Sea gene, one (3.23%) Seb, 17 (54.84%) Spa and seven (22.58%) had PVL. Two MSSA strains (2.98%) expressed the Sea gene, three (4.48%) Seb, 18 (26.87%) Spa and 11 (16.42%) showed positive results for the PVL gene. There was no expression of Sec and CflA between MRSA and MSSA strains. Among MRSA and MSSA isolates, none statistical differences were observed in biofilm production. The analysis of cytokine induction in the inflammatory response of J774 macrophages by MRSA and MSSA isolates did not show statistical difference. Understanding the mechanisms of pathogenesis of S. aureus could provide important clues for both preventing and treating infection caused by these organisms.

关键词： MRSA MSSA virulence factor Hospitalar Infection

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Two-component signal transduction systems and regulation of virulence factors in Xanthomonas: a perspective

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Frontiers in Biology 2010年第6期5卷 495-506页

作者： Fang-Fang WANG Li WANG Wei QIAN State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Graduate School Chinese Academy of SciencesBeijing 100049China National Plant Gene Research Center Beijing 100101China

Two-component signal transduction systems(TCSTSs),consisting of a histidine kinase and a response regulator,play a critical role in regulating virulence gene expression in Gram-negative phytopathogenic bacteria Xanthomonas spp..To date,12 TCSTS genes have been identiﬁed,accounting for approximately 10%of the TCSTS genes in each genome that have been experimen-tally identiﬁed to be related to *** TCSTSs modulate the expression of a number of virulence factors through diverse molecular mechanisms such as interacting with DNA,protein-binding and involvement in second messenger metabolism,which generates a high level of regulatory *** we summarize the current knowledge in thisﬁeld and discuss the emerging themes and remaining questions that are important in deciphering the signaling network of TCSTSs in Xantho-monas.

关键词： Xanthomonas two-component signal trans-duction system virulence factor

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The Group A Streptococcus Interleukin-8 Protease SpyCEP Promotes Bacterial Intracellular Survival by Evasion of Autophagy

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Infectious Microbes & Diseases 2022年第3期4卷 116-123页

作者： RenéBergmann Giuseppe Gulotta Federica Andreoni Tomoko Sumitomo Shigetada Kawabata Annelies S.Zinkernagel Gursharan S.Chhatwal Victor Nizet Manfred Rohde Satoshi Uchiyama Central Unit for Microscopy(ZEIM) Helmholtz Centre for Infection ResearchBraunschweigGermany Division of Infectious Diseases and Hospital Epidemiology University Hospital ZurichZurichSwitzerland Department of Oral and Molecular Microbiology Osaka University Graduate School of DentistrySuita-OsakaJapan Division of Host-Microbe Systems and Therapeutics Department of PediatricsUniversity of CaliforniaSan DiegoLa JollaCalifornia

Autophagy serves an innate immune function in defending the host against invading bacteria,including group A Streptococcus(GAS).Autophagy is regulated by numerous host proteins,including the endogenous negative regulator calpain,a cytosolic *** disseminated serotypeM1T1 GAS strains associated with high invasive disease potential express numerous virulence factors and resist autophagic *** in vitro infection of human epithelial cell lines with representative wild-type GAS M1T1 strain 5448(M1.5448),we observed increased calpain activation linked to a specific GAS virulence factor,the interleukin-8 protease *** activation inhibited autophagy and decreased capture of cytosolic GAS in *** contrast,the serotype M6 GAS strain JRS4(***4),which is highly susceptible to host autophagy-mediated killing,expresses low levels of SpyCEP and does not activate *** of SpyCEP in ***4 stimulated calpain activation,inhibited autophagy,and significantly decreased bacterial capture in *** paired loss-and gain-of-function studies reveal a novel role for the bacterial protease SpyCEP in enabling GAS M1 evasion of autophagy and host innate immune clearance.

关键词： group A Streptococcus SpyCEP virulence factor epithelial cells intracellular survival autophagy xenophagy calpain

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Reprogramming of phytopathogen transcriptome by a non-bactericidal pesticide residue alleviates its virulence in rice

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Fundamental Research 2022年第2期2卷 198-207页

作者： Haruna Matsumoto Yuan Qian Xiaoyan Fan Sunlu Chen Yanxia Nie Kun Qiao Dandan Xiang Xinzhong Zhang Meng Li Bo Guo Peilin Shen Qiangwei Wang Yunlong Yu Tomislav Cemava Mengcen Wang State Key Laboratory of Rice Biology&Ministry of Agricultural and Rural Affairs Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of Pesticide and Environmental ToxicologyZhejiang UniversityHangzhou 310058China Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China Institute of Environmental Biotechnology Graz University of TechnologyPetersgasse 128010 GrazAustria Global Education Program for AgriScience Frontiers Graduate School of AgricultureHokkaido UniversitySapporo 0608589Japan State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095China Ecology and Environmental Sciences Center South China Botanical GardenChinese Academy of SciencesGuangzhou 510650China Key laboratory of South Subtropical Fruit Biology and Genetic Resource Uilization(MOA) Instiute of Fruit Tree ResearchGuangdong Acadermy of Agricultural SciencesGuangzhou 510640China Tea Research Instiute Chinese Academy of Agriculural SciencesHangzhou 310008China Shanghai Intermnational Studies University Shanghai 200083China Xiaoshan Agricultural Comprehensive Development Zone&Management Committee Hangzhou 311200China

Bacteria equipped with virulence systems based on highly bioactive small molecules can circumvent their host's defense *** employing this strategy are currently threatening global rice *** the present study,variations in the virulence of the highly destructive Barkholderiaplantarii were observed in different rice-producing *** environment-linked variation was not attributable to any known host-related or external ***-occurrence analyses indicated a connection between reduced virulence and 5-Amino-l,3,4-thiadiazole-2-thiol(ATT),a non-bactericidal organic ***,which accumulates in rice plants during metabolization of specific agrochemicals,was found to reduce virulence factor secretion by *** up to 88.8%and inhibit pathogen virulence by hijacking an upstream signaling *** assessment of the newly discovered virulence inhibitor resulted in mechanistic insights into positive effects of ATT accumulation in plant *** of virulence alleviation were deciphered by integrating high-throughput data,gene knockout mutants,and molecular interaction ***,a histidine protein kinase in a two-component system that regulates virulence factor secretion,is likely the molecular target antagonized by *** findings provide novel insights into virulence modulation in an important plant-pathogen system that relies on the host's metabolic activity and subsequent signaling interference.

关键词： Phytopathogen virulence factor Transcriptome reprogramming Agrochemical Pesticide Rice

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virulence Phenotyping and Molecular Characterization of a Low-pathogenicity Isolate of Listeria monocytogenes from Cow's Milk

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Acta Biochimica et Biophysica Sinica 2006年第4期38卷 262-270页

作者： Ling-Li JIANG Jing-Jing XU Ning CHEN Jiang-Bing SHUAI Wei-Huan FANG* Institute of Preventive Veterinary Medicine,Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine,Zhejiang University,Hangzhou 310029,China Institute of Preventive Veterinary Medicine Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine Zhejiang UniversityHangzhou 310029 China

A low-pathogenicity isolate of Listeria monocytogenes from cow’s milk,as screened in mouseand chicken embryonated egg models,was examined for virulence-related phenotypic *** genes (iap,prfA,plcA,hly,mpl,actA,plcB,InlA and InlB) were compared with *** strains 10403S and EGD to elucidate the possible molecular mechanisms of low ***-though *** H4 exhibited similar patterns to strain 10403S in terms of hemolytic activity,invitro growth and invasiveness and even had higher adhesiveness,faster intracellular growth and higherphospholipase activity in vitro,it was substantially less virulent than the strain 10403S in mouse and chickenembryo models (50% lethal dose:10vs.10and 10vs.10,respectively).The genes prfA,plcA andmpl were homologous among *** strains H4,10403S and EGD (>98%).Genes iap,hly,plcB,InlA and InIB of *** 10403S had higher homology to those of strain EGD (>98%) than *** homology of the gene hly between strain 10403S and isolate H4 was 96.9% at the nucleotide level,but 98.7% at the amino acid *** actA gene of isolate H4 had deletions of 105 nucleotides correspondingto 35 amino acid deletions falling Within the proline-rich *** together,this study presents someclues as to reduced virulence to mice and chicken embryos of the isolate H4 probably as a result of deletionmutations of actA.

关键词： Listeria monocytogenes virulence factor virulence-associated gene

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Genomic characterization and antimicrobial susceptibility of bovine intrauterine Escherichia coli and its relationship with postpartum uterine infections

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Journal of Integrative Agriculture 2016年第6期15卷 1345-1354页

作者： YANG Li-ming WANG Yi-hao PENG Yu MIN Jiang-tao HANG Su-qin ZHU Wei-yun Laboratory of Gut Microbiology Nanjing Agricultural University

To investigate the roles of Escherichia coli in the pathogenesis of postpartum uterine diseases in dairy cows, a total of 145 E. coli isolates were recovered from 18 healthy cows （61 isolates） and 25 cows with clinical endometritis （84 isolates） at 25-35 days after parturition. Genomic characteristics including phylogenetic grouping, genetic diversity and virulence genes of E. coli isolates were screened to profile the characteristics related to uterine infections. The susceptibility of the bacteria against 23 antibiotics was also evaluated to support prevention and treatment of clinical cases. Genetic diversity of E. coli identified by random amplification of polymorphic DNA （RAPD） revealed 103 clonal types, including 3 common types to unaffected cows and endometritis cows, 39 types specific to healthy cows and 61 types in endometritis subjects. In addition, the isolates from endometritis uteri showed more genetic variability compared with that of healthy cows. Ac- cording to the findings of phylogenetic grouping, the E. coil isolates were assigned to group A （35.9%）, B1 （59.3%） and D （4.8%）. The expression of 10 of 20 virulence gens were detected positively, and only fimH gene was revealed significantly （P〈0.05） associated with endometritis. From antimicrobial susceptibility test, E. coli was found highly resistant to tetracy- cline, ampicillin, carbenicillin and amoxicillin, but sensitive to amikacin, netilmicin, tobramycin, cefepime and ceftazidime. In conclusion, E. cofiwere extensively observed in both healthy and endometritis cows, and presented a large clonal types, however, fimH was the only gene observed associated with clinical endometritis. Our results suggest that the drugs like amikacin, netilmicin, tobramycin and cefepime could be considered for preventing and treating clinical endometritis in the practical management of dairy cow.

关键词： postpartum dairy cow phylogenetic grouping genetic diversity virulence factor antimicrobial susceptibility

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Inhibition of Botrytis cinerea and control of gray mold on table grapes by calcium propionate

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Food Quality and Safety 2021年第3期5卷 279-290页

作者：孙晨旭朱传玺唐瑛任丹丹蔡云飞周港涵王奕文许玲朱品宽 School of Life Sciences East China Normal UniversityShanghaiChina

Objectives:The gray mold fungus Botrytis cinerea(***)infects a wide range of crops before and after harvest,causing huge losses *** mechanisms of *** in vitro and in plants by calcium propionate(CP),generally recognized as a safe substanee,are described in this *** and methods:Wild-type and transgenic mutant strains of *** were used in the study to evaluate the effects of CP on fun gal growth and developme nt in *** materials in cludi ng tomato leaves and table grapes were tested for con trolling efficie ncy of CP agai nst gray mold deterioration in ***:Mycelial growth of *** was inhibited by CP in a dose-dependent manner with occasional disruption of hyphal tips,causing cellular collapse and efflux of cell *** with fluorescein diacetate and propidium iodide indicated that CP decreased fungal cell *** efficiency of CP against *** was enhaneed by reducing *** contrast,the veil mutant,which exhibited deficiency in acid production,was more resista nt to CP,suggesti ng that inhibition of *** by CP is enhanced by the acidification ability of the fungus ***,CP inhibited infection cushion development by germlings of *** assays with tomato leaves and table grapes showed that CP inhibited decay development in both host ***,application of CP on grapes 3 days prior to harvest could contribute to management of deterioration caused by spontaneous fungal diseases during ***:CP can suppress hyphal growth,inhibit infection cushion development,and reduce the virulence of *** is thus promising for practical management of gray mold in fruit crops and merits further evaluation.

关键词： Necrotrophic pathogen virulence factor infection cushion green fluorescent protein generally recognized as safe.

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A Global Transcriptional Regulatormodulates Production of Multiple virulence factors in Dickeyazeae EC1

A Global Transcriptional Regulatormodulates Production of Mu...

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中国植物病理学会2016年学术年会

作者： ZHOU Jia-nuan LV Ming-fa TANG Ying-xin ZHAO Gang CHEN Shao-hua HE Fei CUI Zi-ning JIANG Zi-de CHANG Chang-qing ZHANG Lian-Hui Guangdong Province Key Laboratory of Microbial Signals and Disease Control Department of Plant PathologySouth China Agricultural University Institute of Molecular and Cell Biology

Transcriptional regulators play crucial roles in modulation of virulence factor production in *** this study,we have characterized a SlyA/MarR family transcription factor SlyA in Dickeya zeae strain EC1,the causal agent of rice root rot ***-frame deletion of slyA significantly decreased the production of phytotoxinzeamines,changed the expression patterns of cell wall degrading enzymes,enhanced bacterial motility,reduced biofilm formation,and attenuated the pathogenicityof strain EC1 on host *** analysis showed that SlyA modulates the expression of about 25%of the genome,including the genes encoding various pathogenesis-related proteins,five types of secretion systems and quorum sensing *** addition,based on the transcriptome data,we explored the regulatory pathway of *** results showed that in trans expressionof slyA in the AHL QS mutant expIrecovered the phenotypes of motility and biofilm formation,and in trans expressionof a newly identified regulator genein the slyA mutant rescued the phenotypes of zeamines and the production of cell wall degrading *** together,the findings from this study unveiled a key transcriptional regulatory network involved in modulation of virulence factor production and overall pathogenicity of *** EC1.

关键词： Dickeya zeae EC1 virulence factor Zeamines

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