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Clinical significance of extended high-risk human papillomavirus genotyping and Viral Load in cervical cancer and precancerous lesions

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Gynecology and Obstetrics Clinical Medicine 2023年第1期3卷 22-29页

作者： Pingping Su Jincheng Ma Lirui Yu Shuting Tang Pengming Sun Laboratory of Gynecologic Oncology Department of GynecologyFujian Maternity and Child Health HospitalCollege of Clinical Medicine for Obstetrics&Gynecology and PediatricsFujian Medical UniversityFuzhou350001FujianChina Fujian Key Laboratory of Women and Children's Critical Diseases Research Fujian Maternity and Child Health HospitalAffiliated Hospital of Fujian Medical UniversityFuzhouFujian350001China Fujian Clinical Research Center for Gynecological Oncology Fujian Maternity and Child Health Hospital(Fujian Obstetrics and Gynecology Hospital)Fuzhou350001FujianChina

Persistent infections with specific high-risk human papillomavirus(HR-HPV)strains are the leading cause of cervical cancer and precancerous lesions.HPV-16 and HPV-18 are associated with more than 70%of cervical cancer.However,with recent widespread vaccination efforts against cervical cancer,the infection rates of HPV-16 and HPV-18 have decreased across all age groups,while the infection rates of other HR-HPV strains have increased.The non-16/18 HR-HPV strains play an important role in cervical lesions.These strains can be identified with extended genotyping,and the 2019 American Society for Colposcopy and Cervical Pathology(ASCCP)guidelines recommended an HPV-based testing to assess the risk of cervical disease in patients.We reviewed and analyzed the clinical benefits of applying extended HR-HPV genotyping,which was published by the International Agency for Research on Cancer(HPV-16,18,31,33,35,39,45,51,52,56,58,59,66,and 68),to cervical cancer screening.This review concluded that cervical cancer screening needs to include extended HR-HPV genotyping.The examination of extended HR-HPV genotyping in cervical intraepithelial lesions and cervical cancers can help guide clinical practices.

关键词： High-risk human papillomavirus Cervical cancer Genotype Viral Load Cancer screening

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Human bocavirus infection in children hospitalized with lower respiratory tract infections:Does Viral Load affect disease course?

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Asian Pacific Journal of Tropical Medicine 2022年第8期15卷 354-360页

作者： Ayşe Karaaslan CerenÇetin Serap Demir Tekol Ufuk Yükselmiş Mehmet Tolga Köle Yasemin Akın Department of Pediatric Infectious Diseases University of Health SciencesKartal Dr.Lütfi Kırdar City HospitalIstanbulTurkey Department of Microbiology University of Health SciencesKartal Dr.Lütfi Kırdar City HospitalIstanbulTurkey Department of Pediatric Intensive Care Unit University of Health SciencesKartal Dr.Lütfi Kırdar City HospitalIstanbulTurkey Department of Pediatrics University of Health SciencesKartal Dr.Lütfi Kırdar City HospitalIstanbulTurkey

Objective:To examine the effects of human bocavirus type 1(HBoV1)on the course of lower respiratory tract infections in cases of monoinfection and coinfection,and the effects of HBoV1 Viral Load on the disease in children under six years old hospitalized with a diagnosis of HBoV1-associated lower respiratory tract infections.Methods:Children under six years of age,who were hospitalized with the diagnosis of lower respiratory tract infection due to HBoV1 between 1 January 2021 and 1 January 2022 were included in the study.Laboratory confirmation of the respiratory pathogens was performed using polymerase chain reaction(PCR).Results:Fifty-four(16.4%)children with HBoV1 among 329 children whose PCR was positive with bacterial/viral agent in nasopharyngeal swab samples were included in the study.There were 28(51.9%)males and 26(48.1%)females with a median age 23.4 months[interquartile range(IQR):13.2,30.0 months](min-max:1 month-68 months).HBoV1 was detected as a monoinfecton in 26(48.1%)children,and as a coinfection with other respiratory agents in 28 children(51.9%).In multiple regression analysis,coinfection(P=0.032)was associated with the length of hospitalization(PViral Load in children with more cough symptoms in our study,it had no effect on the severity of the disease,such as length of hospital stay and need for intensive care.Coinfection was found to affect the length of hospitalization.

关键词： Human bocavirus Lower respiratory tract infection Children Viral Load

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Stroke and HIV: Correlation between Viral Load and Type of Stroke

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Neuroscience & Medicine 2021年第4期12卷 163-167页

作者： Prince Eliot Galieni Sounga Bandzouzi Ghislain Armel Mpandzou Josué Euberma Diatewa Patience Moudeko M’Foutou Dina Happia Motoula-Latou Charles Godefroy Koubemba Paul Macaire Ossou-Nguiet Donatien Moukassa Faculty of Health Sciences Marien N’GOUABI University Brazzaville Republic of Congo Department of Neurology Loandjili General Hospital Pointe-Noire Republic of the Congo Department of Neurology University Hospital of Brazzaville Brazzaville Republic of Congo

Introduction: The role of immunosuppression of TCD4+ lymphocytes in the onset of stroke in people living with HIV has been reported in numerous studies examining the co-morbidity of stroke and HIV. Objective: To determine the correlation between the Viral Load and the type of stroke. Methodology: This was a 7-month cross-sectional descriptive study carried out in the Neurology Department of Loandjili General Hospital in Pointe-Noire. The study population consisted of patients living with HIV who had a stroke confirmed by brain scan. The sero-immunological investigation consisted of looking for T lymphocyte typing from two kits: a CD4+ T lymphocyte typing reagent kit (BD FACS Presto TM) and a GeneXpert kit for Viral Load (Xpert®HIV-1 Viral Load). The database was made from the 2010 version of Microsoft Excel. Results: We included 16 patients living with HIV, 56% of whom were women with a sex ration of 0.78. The mean age was 56.92 ± 11.21. The mean number of TCD4+ lymphocytes was 413.44 ± 677.95/mm3;minimum: 93/mm3;maximum: 2854/mm3. The mean Viral Load was 17,996.31 ± 20,982.22/mm3;minimum: 1002/mm3;maximum: 67,229/mm3. No significant difference between the Viral Load and the occurrence of the stroke (p = 0.13). Conclusion: Our study did not show a causal link between Viral Load, immunosuppression of TCD4+ lymphocytes and the onset of stroke.

关键词： Viral Load HIV Stroke

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Correlation between Viral Load and liver cirrhosis in chronichepatitis B patients

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Frontiers of Medicine 2009年第3期3卷 271-276页

作者： Lili LIU Jiyao WANG Weimin SHE Department of Gastroenterology and Hepatology Zhong Shan HospitalFudan UniversityShanghai 200032China

The aim of this paper is to investigate the relationship between hepatitis B virus(HBV)DNA levels during the course and the progression to cirrhosis with chronic hepatitis B.A total of 239 chronic hepatitis B patients conﬁrmed by liver biopsy between 2001 and 2007 were followed up for a median of 28 months.Compared with the patients without cirrhosis,the patients progressed to cirrhosis were older and with higher HBV-DNA levels at end point.However,there was no signiﬁcant difference in cirrhosis progression between different HBV-DNA groups at baseline(P=0.531).Kaplan-Meier analysis showed higher HBV-DNA level at endpoint had increasing risk of cirrhosis(P=0.019).The results of Cox model indicated that HBV-DNA levels at endpoint,stage ofﬁbrosis,negative hepatitis B e antigen,andγ-glutamyl transpepti-dase at baseline were independent risk factors of cirrhosis.The relative risk ratios were 1.898,1.918,8.976,and 1.006,respectively.Progression to cirrhosis in chronic hepatitis B patients is correlated with HBV-DNA levels during follow-up.

关键词： hepatitis B,chronic Viral Load liver cirrhosis

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Co-relation of SARS-CoV-2 related 30-d mortality with HRCT score and RT-PCR Ct value-based Viral Load in patients with solid malignancy

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World Journal of Clinical Oncology 2022年第5期13卷 339-351页

作者： Satya Narayan Vineet Talwar Varun Goel Krushna Chaudhary Anurag Sharma Pallavi Redhu Satyajeet Soni Arpit Jain Department of Medical Oncology Rajiv Gandhi Cancer Institute and Research CentreNew Delhi 110085India Department of Research Rajiv Gandhi Cancer Institute and Research CentreNew Delhi 110085India

BACKGROUND Coronavirus disease 2019(COVID-19)patients with malignancy are published worldwide but are lacking in data from India.AIM To characterize COVID-19 related mortality outcomes within 30 d of diagnosis with HRCT score and RT-PCR Ct value-based Viral Load in various solid malignancies.METHODS Patients included in this study were with an active or previous malignancy and with confirmed severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection from the institute database.We collected data on demographic details,baseline clinical conditions,medications,cancer diagnosis,treatment and the COVID-19 disease course.The primary endpoint was the association between the mortality outcome and the potential prognostic variables,specially,HRCT score,RT-PCR Ct value-based Viral Load,etc.using logistic regression analyses treatment received in 30 d.RESULTS Out of 131 patients,123 met inclusion criteria for our analysis.The median age was 57 years(interquartile range=19-82)while 7(5.7%)were aged 75 years or older.The most prevalent malignancies were of GUT origin 49(39.8%),hepatopancreatobiliary(HPB)40(32.5%).109(88.6%)patients were on active anticancer treatment,115(93.5%)had active(measurable)cancer.At analysis on May 20,2021,26(21.1%)patients had died.In logistic regression analysis,independent factors associated with an increased 30-d mortality were in patients with the symptomatic presentation.Chemotherapy in the last 4 wk,number of comorbidities(≥2 vs none:3.43,1.08-8.56).The univariate analysis showed that the risk of death was significantly associated with the HRCT score:for moderate(8-15)[odds ratio(OR):3.44;95%confidence interval(CI):1.3-9.12;P=0.0132],severe(>15)(OR:7.44;95%CI:1.58-35.1;P=0.0112).CONCLUSION To the best of our knowledge,this is the first study from India reporting the association of HRCT score and RT-PCR Ct value-based 30-d mortality outcomes in SARS-CoV-2 infected cancer patients.

关键词： SARS-CoV-2 COVID-19 Cancer HRCT Viral Load

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Performance evaluation of NeuMoDx 96 system for hepatitis B and C Viral Load

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World Journal of Virology 2023年第4期12卷 233-241页

作者： Gagan Chooramani Jasmine Samal Nitiksha Rani Gaurav Singh Reshu Agarwal Meenu Bajpai Manoj Kumar Manya Prasad Ekta Gupta Department of Clinical Virology Institute of Liver&Biliary SciencesNew Delhi 110070India Department Transfusion Medicine Institute of Liver&Biliary SciencesNew Delhi 110070India Department of Hepatology Institute of Liver and Biliary SciencesNew Delhi 110070India Department of Epidemiology and Clinical Research Institute of Liver and Biliary SciencesNew Delhi 110070India

BACKGROUND Hepatitis B virus(HBV)and hepatitis C virus(HCV)Viral Load(VL)estimation is essential for the management of both HBV and HCV infections.Due to a longer turnaround time for VL estimation,many patients drop out from the cascade of care.To achieve the global goals of reducing morbidity and mortality due to HBV/HCV and moving towards their elimination by 2030,molecular diagnostic platforms with faster and random(i.***.single sample)access are needed.AIM To evaluate the performance of the recently launched NeuMoDx 96 random access system with the conventional COBAS^(■)AmpliPrep/COBAS TaqMan system for HBV and HCV VL estimation.METHODS Archived once-thawed plasma samples were retrieved and tested on both platforms.Correlation between the assays was determined by linear regression and Bland-Altman analysis.The study included samples from 186 patients,99 for HBV of which 49 were true infected HBV cases(hepatitis B surface antigen,antihepatitis B core antibody,and HBV DNA-positive)and 87 for HCV assay in which 39 were true positives for HCV infection(anti-HCV and HCV RNA-positive).RESULTS The median VL detected by NeuMoDx for HBV was 2.9(interquartile range[IQR]:2.0-4.3)log_(10)IU/mL and by COBAS it was 3.70(IQR:2.28-4.56)log_(10)IU/mL,with excellent correlation(R2=0.98).In HCV,the median VL detected by NeuMoDx was 4.9(IQR:4.2-5.4)log_(10)IU/mL and by COBAS it was 5.10(IQR:4.07-5.80)log_(10)IU/mL with good correlation(R2=0.96).CONCLUSION The overall concordance between both the systems was 100%for both HBV and HCV VL estimation.Moreover,no genotype-specific bias for HBV/HCV VL quantification was seen in both the systems.Our findings reveal that NeuMoDx HBV and HCV quantitative assays have shown overall good clinical performance and provide faster results with 100%sensitivity and specificity compared to the COBAS AmpliPrep/COBAS TaqMan system.

关键词： Hepatitis B Hepatitis C NeuMoDx Random access Viral Load COBAS AmpliPrep

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Evolution of Viral Load in Patients Infected with HIV-1 at Point G University Hospital

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Open Journal of Medical Microbiology 2024年第1期14卷 66-76页

作者： A. Maiga D. Kone D. M. Coulibaly Ag M. Baraika A. Traore S. S. Diakite I. I. Maiga I. Konate A. I. Maiga Faculty of Medicine and Odontostomatology of Bamako (FMOS) University of Science of Technical and Technology De Bamako Bamako Mali Laboratory of Medical Biology and Hospital Hygiene of Point-G University Hospital Bamako Mali Faculty of Pharmacy of Bamako (FaPh) University of Science of Technical and Technology De Bamako Bamako Mali Laboratory of the Luxembourg Mother-Child Hospital Bamako Mali National Institute of Public Health Bamako Mali National Institute for Training in Health Sciences Bamako Mali Laboratory of the Kati University Hospital Center Bamako Mali Infectious and Tropical Diseases Department of Point-G University Hospital Bamako Mali Laboratory of CHU Gabriel Touré Bamako Mali

Introduction: HIV, the human immunodeficiency virus, is the etiological agent of acquired immunodeficiency syndrome (AIDS). The aim of this study was to assess the evolution of the Viral Load in patients under treatment. Methodology: This was a study carried out from July 2017 to June 2022 at the Point G University Hospital laboratory. The determination of the Viral Load of patients was carried out by PCR on the ABOTT M2000sp/rt platform. Results: A total of 129 patients infected with HIV-1, aged 19 to 72 years with a mean age of 40.05 years ± 10.71;all on antiretroviral chemotherapy. The female gender predominated among our patients. The most common treatment regimen was 2INTI + 1INNTI with 72.9% followed by 2INTI + 1INI with 13.2%. As for the combinations of molecules, the combination TDF + 3TC + EFV and TDF + 3TC + DTG predominated, respectively 65.1% and 13.2%. 89.9% of our patients had undetectable viremia after 12 months of treatment (p Viral Load which had evolved from 681315.65 copies/ml ± 1616908.484 to M0 at 5742.36 copies /ml ± 35756.883 at M12 (p Conclusion: Generally speaking, antiretroviral treatment had contributed to controlling Viral Loads, however the therapeutic combination TDF + 3TC + DTG had made it possible to obtain more patients with undetectable viremia instead.

关键词： HIV-1 Treatment Viral Load Point G University Hospital

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SARS-CoV-2(COVID-19),Viral Load and clinical outcomes;lessons learned one year into the pandemic:A systematic review

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World Journal of Critical Care Medicine 2021年第4期10卷 132-150页

作者： Santosh Shenoy Department of General and Colorectal Surgery KCVA and University of Missouri at Kansas CityMissouriMO 64128United States

BACKGROUND Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infections is diagnosed via real time reverse transcriptase polymerase chain reaction(RT-PCR)and reported as a binary assessment of the test being positive or negative.High SARS-CoV-2 Viral Load is an independent predictor of disease severity and mortality.Quantitative RT-PCR may be useful in predicting the clinical course and prognosis of patients diagnosed with coronavirus disease 2019(COVID-19).AIM To identify whether quantitative SARS-CoV-2 Viral Load assay correlates with clinical outcome in COVID-19 infections.METHODS A systematic literature search was undertaken for a period between December 30,2019 to December 31,2020 in PubMed/MEDLINE using combination of terms“COVID-19,SARS-CoV-2,Ct values,Log_(10) copies,quantitative Viral Load,viral dynamics,kinetics,association with severity,sepsis,mortality and infectiousness”.After screening 990 manuscripts,a total of 60 manuscripts which met the inclusion criteria were identified.Data on age,number of patients,sample sites,RT-PCR targets,disease severity,intensive care unit admission,mortality and conclusions of the studies was extracted,organized and is analyzed.RESULTS At present there is no Food and Drug Administration Emergency Use Authorization for quantitative Viral Load assay in the current pandemic.The intent of this research is to identify whether quantitative SARS-CoV-2 Viral Load assay correlates with severity of infection and mortality?High SARS-CoV-2 Viral Load was found to be an independent predictor of disease severity and mortality in majority of studies,and may be useful in COVID-19 infection in susceptible individuals such as elderly,patients with co-existing medical illness such as diabetes,heart diseases and immunosuppressed.High Viral Load is also associated with elevated levels of TNF-α,IFN-γ,IL-2,IL-4,IL-6,IL-10 and C reactive protein contributing to a hyper-inflammatory state and severe infection.However there is a wide heterogeneity in fluid samples and different phases

关键词： COVID-19 SARS-CoV-2 Viral Load Severe sepsis Dynamics Mortality

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SARS-CoV-2 Viral Load in the upper respiratory tract and disease severity in COVID-19 patients

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World Journal of Meta-Analysis 2022年第4期10卷 195-205页

作者： Wattana Leowattana Tawithep Leowattana Pathomthep Leowattana Department of Clinical Tropical Medicine Faculty of Tropical MedicineMahidol UniversityBangkok 10400Thailand Department of Medicine Faculty of MedicineSrinakharinwirot UniversityBangkok 10110Thailand

Due to the disease's broad clinical spectrum,it is currently unclear how to predict the future prognosis of patients at the time of diagnosis of coronavirus disease 2019(COVID-19).Real-time reverse transcription-polymerase chain reaction(RTPCR)is the gold standard molecular technique for diagnosing COVID-19.The number of amplification cycles necessary for the target genes to surpass a threshold level is represented by the RT-PCR cycle threshold(Ct)values.Ct values were thought to be an adequate proxy for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Viral Load.A body of evidence suggests that SARS-CoV-2 Viral Load is a possible predictor of COVID-19 severity.The link between SARS-CoV-2 Viral Load and the likelihood of severe disease development in COVID-19 patients is not clearly elucidated.In this review,we describe the scientific data as well as the important findings from many clinical studies globally,emphasizing how Viral Load may be related to disease severity in COVID-19 patients.Most of the evidence points to the association of SARS-CoV-2 Viral Load and disease severity in these patients,and early anti-viral treatment will reduce the severe clinical outcomes.

关键词： Severe acute respiratory syndrome coronavirus-2 Viral Load Upper respiratory tract Coronavirus disease 2019 patients Disease severity Clinical outcome

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Profiling Hepatitis B Viral Load: Treatment and Epidemiological Implications in a West African Hospital

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Advances in Infectious Diseases 2022年第4期12卷 703-714页

作者： Papa Silman Diawara Mor Ngom Maguette Ndoye Mamadou Wague Gueye Sokhna M. Daffe Nata Dieng Ibrahima Diallo Bécaye Fall Macoura Gadji Moustapha Diop Pape Samba Ba Yankhoba Diop Federation of Laboratories Principal Hospital of Dakar Dakar Senegal Hematology Department Dakar Principal Hospital Dakar Senegal Hepato-Gastro-Enterology Department Dakar Principal Hospital Dakar Senegal Department of Biological Hematology & Oncology-Hematology (HBOH) National Blood Transfusion Center (CNTS)/FMPOS-UCAD Dakar Senegal Infectious Diseases Department Dakar Principal Hospital Dakar Senegal

Background: Chronic hepatitis B virus (HBV) infection is one of the largest public health problems with nearly 350 million chronic carriers and 500,000 deaths each year. These deaths are most often associated with disease progression to cirrhosis or hepatocellular carcinoma, which some studies have shown is associated with long-term viral replication in chronic carriers. Viral Load quantification, a key element of disease management, is expensive and difficult to access. Viral Load plays a crucial role in patient classification and treatment initiation. Four years after the implementation of Viral Load platform, the objective of this study was to assess Viral Load profile in HBs chronic carriers in a sub-Saharan Hospital and to determine the potential impact of this distribution on preventive and therapeutic strategies against hepatitis B infection. Materials and Method: The study was carried out between April 2016 and October 2020 in the laboratory of the PRINCIPAL Hospital in Dakar. All patients referred for HBV DNA Viral Load testing following a positive AgHBs test were included. Incomplete medical records were excluded from the study. Only the first quantification test performed on each patient is recorded. DNA extraction was performed with COBAS AmpliPrep (Roche Molecular Systems, Inc., Branchburg, NJ, USA). Amplification was performed using COBAS TaqMan48 (Roche Molecular Systems, Inc., Branchburg, NJ, USA). Data were collected from the laboratory’s computer system and entered into Microsoft Excel (2007). Statistical analyzes were performed using Epi-Info 7 software. Results: A total of 3002 patients, 76.1% (2285/3002) men and 33.9% (717/3002) women, were included in the study. Young adults were most represented among the subjects (23.2%) and (20.1%) in the age groups 25 - 30 and 30 - 35. The majority (52.7%) of patients had Viral Loads between 20 and 2000 IU. Patients with undetectable Viral Loads and patients with Viral Loads below 20 IU comprised 14.6% and 7.53% of the study population, respectively. Pat

关键词： Hepatitis B Viral Load Senegal Treatment

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