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Screening of marine seaweeds for bioactive compound against fish pathogenic bacteria and active fraction analysed by gas chromatography-mass spectrometry

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Journal of Coastal Life Medicine 2014年第5期 367-375页

作者： Rajasekar Thirunavukkarasu Priyadharshini Pandiyan Kumaran Subaramaniyan Deivasigamani Balaraman Sakthivel Manikkam Balamurugan Sadaiyappan George Edward Gnana Jothi Centre of Advance Study in Marine Biology Faculty of Marine SciencesAnnamalai UniversityParangipettai-608508Tamil NaduIndia Sri Sankara Arts and Science College EnathurKanchipuram-631561Tamil NaduIndia

Objective:To isolate bioactive molecules from marine seaweeds and check the antimicrobial activity against the fish pathogenic ***:Fresh marine seaweeds Gracilaria edulis,Kappaphycus spicifera,Sargassum wightii(***)were *** seaweed was extracted with different *** the study,test pathogens were collected from microbial type culture *** activity was carried out by using disc diffusion method and minimum inhibition concentration(MIC)was *** seaweed was analysed by fourier transform infrared *** cured extract was separated by thin layer chromatography(TLC).Fraction was collected from TLC to check the antimicrobial *** fraction was analysed by gas chromatography mass spectrometer(GCMS).Results:Based on the disc diffusion method,*** showed a better antimicrobial activity than other seaweed *** on the MIC,methanol extract of *** showed lower MIC than other *** were separated by *** this TLC,plate showed a two *** two fractions were separated in preparative TLC and checked for their antimicrobial *** 2 showed best MIC value against the tested *** 2 was analysed by *** on the GCMS,fraction 2 contains n-hexadecanoic acid(59.44%).Conclusions:From this present study,it can be concluded that *** was potential sources of bioactive compounds.

关键词： Marine seaweeds vibrio sp.fish pathogenic Thin layer chromatography Gas chromatography-mass spectrometry

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