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Selection of the appropriate reference genes by quantitative real-time PCR in leopard coral groupers Plectropomus leopardus

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Journal of Oceanology and Limnology 2023年第3期41卷 1084-1099页

作者： Han ZHANG Haoran YANG Pengsuo LI Na ZHAO Xiaojuan CHEN Yongcan ZHOU Zhenjie CAO Yun SUN State Key Laboratory of Marine Resource Utilization in South China Sea Hainan UniversityHaikou 570228China Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province Haikou 570228China Department of Aquaculture College of Marine SciencesHainan UniversityHaikou 570228China

Leopard coral groupers(Plectropomus leopardus),commercially bred in South China,are a significant economical fish *** this study,by means of quantitative real-time PCR(qRT-PCR)technology,we assessed the stability of six common reference genes expression and selected the appropriate reference genes in leopard coral groupers with or without vibrio harveyi stimulation at different time *** data produced by qRT-PCR was handled via the geNorm,NormFinder,and BestKeeper *** results revealed all the examined reference genes had manifest tissue-specific expression in different *** to *** stimulation,RPL13 gene was the appropriate reference gene among eleven tissue types(blood,spleen,hepatopancreas,kidney,stomach,gill,heart,skin,muscle,intestine,brain)in leopard coral *** *** stimulation,the most reliable reference genes varied from tissue to tissue and were closely hinged upon different time *** 6-h post-bacterial injection,the appropriate reference genes in hepatopancreas,spleen,kidney,and gill were Actin,B2M,UBCE,and Actin,*** 9-and 12-h post-bacterial injection,the appropriate reference genes in hepatopancreas,spleen,kidney,and gill were RPL13,Actin,Actin,and Actin,*** one reference gene was preferable,RPL13,Actin,Actin,and Actin could be selected as the reference gene in hepatopancreas,spleen,kidney,and gill of leopard coral groupers after *** infection,*** profiles of two target genes(IL-6 and NK-lysin)were used to further validate reliability of these selected appropriate *** study will lay a solid foundation for the future research on qRTPCR analysis of gene expression in leopard coral groupers.

关键词： Plectropomus leopardus reference genes vibrio harveyi stimulation

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