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Detection of verotoxigenic Ecoli in animal products

Detection of verotoxigenic Ecoli in animal products

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中国畜牧兽医学会2010年学术年会——第二届中国兽医临床大会

作者： Rashad Alkasir China Agricultural University College of Veterinary Medicine

verocytotoxin-producing escherichia coli(VTEC) is recognized as an important food-borne pathogen,responsible for sporadic cases to serious outbreaks *** morbidity and mortality associated with several recent outbreaks due to VTEC have highlighted the threat of this organism poses to public *** its discovery in 1982,the organism has been the focus of extensive research on its epidemiology,pathogenesis,diagnosis,*** most important virulent attribute of the VTEC is Verotoxin, The organism may cause serious human complications like Haemolytic Uraemic Syndrome and Haemorrhagic *** animals,especially cattle and sheep,are the main reservoirs and sources of *** meat and meat products,dairy products,have been recognized as main vehicles of spread of infection to *** have been many advances in rapid diagnosis of this infection using the current molecular biology,immunological and cytotoxic *** is the first study carried out in Syria to detect VTEC in the most popular animal’s products in the Syrian market(carcasses,hamburger,shawrma, milk,cheese ).Beside we detected the pathogen in the faeces of healthy sheep and cattle to know the role which they play as reservoir to *** collect 750 samples from slaughters,stores,fast food restaurants, faeces from healthy sheep and cattle,in Salamieh and governorate of Hama and *** samples subjected to classical bacteriology examination and isolation applied to isolate *** in general,and *** O157 in particular,all *** isolates then passed through Vero Cell Assay to detect the lethal effect of Vero Toxin on this cell line,and multiplex PCR to detect the Vero Toxin genes in the *** *** isolate 78 strains of VTEC from 750 *** prevalence of VTEC among the samples was 10.4%(VTEC Non-O157:7.9%,VTEC O157:2.5%).the Multiplex-PCR revealed that 35 strains posses vtl,17 strains posses vt2,26 posses the both genes.

关键词： verocytotoxin-producing escherichia coli Verotoxin multiplex PCR

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