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vegetative propagation of Dalbergia sissoo:effect of growth regulators,length,position of shoot and type of cuttings on rooting potential in stem cuttings

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Forestry Studies in China 2012年第3期14卷 187-192页

作者： Bhupendra SINGH Rajendra YADAV B.P. BHATT Department of Forestry H.N.B.Garhwal UniversitySrinagar (Garhwal)Uttarakhand 246174India Chhattisgarh Minor Forest Produce Federation A-25VIP EstateKhamhardihShankar NagarRaipur 492007India ICAR Research Complex for Eastern Region PatnaBihar 800014India

The effect of auxins （IAA or IBA at 100, 200, 500 mg.L J） on rooting and sprouting parameters differed significantly （p 〈 0.05）. Rooting and sprouting percentages were higher in some treatments while other parameters were significantly higher in other treatments. The results of the present study revealed that rooting was significantly （p 〈 0.05） affected by the length of cuttings, as well as the position of shoots and type of cuttings. On average, 86.0 per cent rooting was observed in the 30-cm long cuttings com- pared to the other two lengths of cuttings, i.e., 20 and 25 cm, irrespective of any auxin treatment. In a control set, without any auxin treatment, a maximum of 60.0% rooting was recorded in the 30-cm long cuttings and a minimum of 40.0% of the cuttings rooted in the 20-cm long cuttings. Similarly, cuttings collected from the lower and upper portions of shoots resulted in 72.5 and 52.5 per cent rooting respectively, irrespective of auxin treatments. Maximum rooting was observed in the lower portion （40.0%） of cutting com- pared to the upper portion （30.0%） in the control set without auxin treatment. Leafless branches produced maximum （72.0%） rooting compared to the leafy cuttings （35.0%）, irrespective of the auxin treatment. The leafy cuttings produces a minimum of 15.0% sprout- ing and rooting in the control set.

关键词： auxin Dalbergia sissoo length position of shoots type of cuttings vegetative propagation

Effect of Indoor and Underground Storage on Efficient vegetative propagation of Dwarf Napier Grass (Pennisetum purpureum Schumach)

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American Journal of Plant Sciences 2016年第8期7卷 1173-1179页

作者： Yusuke Iki Yasuyuki Ishii Satoru Fukagawa Sachiko Idota Graduate School of Agriculture University of Miyazaki Miyazaki Japan Faculty of Agriculture University of Miyazaki Miyazaki Japan Nagasaki Agricultural and Forestry Technical Development Center Shimabara Japan

Dwarf, late-heading (DL) Napier grass is suitable for both cut-and-carry and grazing management due to sufficient yield and quality potential. This species can hardly produce viable seed, and thus vegetative propagation should be essential before winter in temperate regions of southern Kyushu. The objective of this study was to determine the efficient storage methods of DL vegetative propagation. Two experiments were carried out, the first focused on indoor storage of 10-node stem sections under room or chilling (10°C) conditions and the second, on underground storage of ten-node and one-node stem sections at 10 cm, 25 cm or 40 cm depth. After both storages, both of ten-node stem sections, cut into single nodes, and one-node stem sections were transplanted into the soil and counted for the emergence every month in 3 months of planting. In indoor-storage, while storage temperature hardly affected the emergence (P > 0.05), storage period significantly reduced the emergence percentage. In underground storage, while one-node storage achieved constantly 60% emergence, ten-node storage achieved a higher emergence at 80% under 25-cm and 40-cm depths. The results of the study suggest that underground storage of longer stem sections at 25 cm depth could be applied to practical vegetative propagation in the region.

关键词： vegetative propagation Temperature Storage Stem Section Dwarf Napier Grass

Successful vegetative propagation by stem cuttings of Tetraena mongolica Maxim.

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Research in Cold and Arid Regions 2009年第5期1卷 423-431页

作者： ZhiGang Zhou GuoHou Liu Fang Luo College of Ecology and Environment Science Inner Mongolia Agricultural University Hohhot Inner Mongolia 010018 China College of Biological Science and Biotechnology Beijing Forestry University Beijing 100083 China

Techniques have been developed for the vegetative propagation of Tetraena mongolica,a rare and endangered species endemic to the western Ordos Plateau in the northcentral portion of *** purpose of the study was to investigate the rooting characteris-tics of the plant species from the aspects of length and ages of cuttings,external hormone concentration,leaf retention,cutting orientations,and cutting *** results obtained from the experiments indicated that the rooting ratio of 2-year or 3-year old cuttings was larger than more than of 4-year old *** influence of external hormone concentration on rooting was ob-vious;the rooting ratio of cuttings was increased by external hormone treatment,especially when treated by ABT1(ABT No.1 Rooting Powder) 50 mg/L for 3 hours,in which case,the rooting ratio reached 91 *** retention influenced rooting sig-nificantly;the rooting rate of cuttings became greater as the leaf retention *** was no significant difference in rooting capability between south and north orientations of the *** rooting ratio of the cuttings obtained from the distal portions of shoots was significantly larger than those obtained from the middle and the basal portions of the *** day three after treat-ment,calluses started appearing at the incisions of some cuttings dipped in 500 mg/L IAA(indole acetic acid).Most cuttings were healed or swelled,and 6 percent of cuttings rooted on day 6 after *** rooting period occurred 7-10 days after the experiment was set up.

关键词： Tetraena mongolica vegetative propagation stem cutting rooting characteristics

Effects of light intensity and photoperiod on runner plant propagation of hydroponic strawberry transplants under LED lighting

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International Journal of Agricultural and Biological Engineering 2019年第6期12卷 26-31页

作者： Jianfeng Zheng Dongxian He Fang Ji Key Laboratory of Agricultural Engineering in Structure and Environment of Ministry of Agriculture and Rural Affairs College of Water Resources and Civil EngineeringChina Agricultural UniversityBeijing 100083China

vegetative propagation of strawberry(Fragaria×ananassa Duch.)in the plant factory with artificial lighting is considered as an effective approach to produce high-quality *** this study,mother plants of‘Benihoppe’strawberry were grown hydroponically for 50 d under eight LED lighting treatments by combining four levels of light intensity(200,250,300 and 350mmol/(m^(2)·s))and two photoperiods(12 h/d and 16 h/d).Runner development,growth of runner plants,photon yield and energy yield in runners and runner plants were investigated to evaluate the strawberry propagation *** indicated that length of runners decreased linearly with increasing daily light integral(DLI)under each photoperiod and was significantly shorter under photoperiod of 16 h/*** elongation was inhibited by high *** of runners and runner plants formed by mother plants increased by 38.9%and 33.7%,when DLI increased from 8.6 to 11.5 mol/(m^(2)·d),respectively;however,no further increase was observed when DLI was higher than 11.5 mol/(m^(2)·d).Similar trends were found in crown diameter and biomass of primary and secondary runner *** impact of high DLI(20.2 mol/(m^(2)·d))on photosynthetic capacity of runner plants was observed as a decrease in leaf net photosynthetic rate,potential maximum photochemical efficiency of PSII,and chlorophyll ***,photon yield and energy yield in runners and runner plants decreased significantly with increasing ***,DLI in a range of 11.5-17.3 mol/(m^(2)·d)is beneficial to improve strawberry propagation efficiency and quality of runner plants,and 11.5 mol/(m^(2)·d)is optimal for the strawberry propagation of runner plants in the LED plant factory because of the higher photon and energy yields.

关键词： hydroponic strawberry vegetative propagation daily light integral photon yield

Effects of indole-3-butyric acid and age of stem cuttings on root morphology,growth,and survival of Cornus sericea

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Journal of Forestry Research 2023年第2期34卷 433-440页

作者： Sahari Inoue Chibuzo Ilogu Jean-Marie Sobze Northern Alberta Institute of Technology Centre for Boreal ResearchPeace RiverAB T8S 1R2Canada Fine Phenotyping Lab Crop Health and ProtectionHarpenden AL52JQUK

Red osier dogwood(Cornus sericea L.),widely distributed throughout North America,is essential for wildlife,thus *** is recommended for reclamation or revegetation of sites disturbed by oil and gas extraction because it tolerates a wide range of soil types and high pH *** germination of this species is extremely difficult with long stratification requirements and poor germination,cuttings facilitate *** this study,to develop techniques to propagate the species from stem cuttings,four concentrations of indole-3-butyric acid(IBA)and two cutting ages previous year’s growth(PYG)and current year’s growth(CYG)were investigated for survival and *** 4 months,survival rate,height growth,and shoot biomass from PYG cuttings were enhanced with IB *** contrast,IB A did not affect these parameters in CYG *** morphology was significantly affected by IB A concentration and cutting ***,at higher concentrations,increased root surface and *** root surface area and length of PYG cuttings were increased,which may facilitate the absorption of essential resources and consequently increase *** results indicate that increasing the concentration of IB A may be an effective way to have better plant survival and growth of previous year’s cuttings in red osier dogwood.

关键词： vegetative propagation IBA Chronological age Cornus sericea L. Survival rate

White oak (Quercus fabri Hance) regenerated stump sprouts show few senescence symptoms during 40 years of growth in a natural forest

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Forest Ecosystems 2021年第1期8卷 139-150页

作者： Honggang Sun Sisheng Wu Liwen Wu Research Institute of Subtropical Forestry Chinese Academy of ForestryHangzhou 311400China

Background:The relationship between physiological age of parental trees and lifespan of clonal offspring is *** oak(Quercus fabri Hance)has a high sprouting capability after harvest,with the regenerated sprouts being typical clonal *** determine whether regenerated sprouts undergo rapid senescence compared with the parent,the senescence levels of 5-,10-,20-and 40-year-old regenerated stump sprouts in a natural forest were *** antioxidative abilities and transcriptomes in these leaves and shoots were ***:Older regenerated sprouts still had robust antioxidative systems,with 40-year-old sprouts having lower peroxidation product levels but similar antioxidative enzyme activity levels compared with 5-year-old *** leaves had greater transcriptional activities in pathways related to cell growth and division than younger ***,older sprouts had some unhealthy characteristics,such as increased base excision repair levels and upregulated phagosome,proteasome and glycerophospholipid metabolism pathways in 40-year-old leaves,which indicates that DNA damage and tissue remodeling occurred more frequently than in younger ***,plant-pathogen interactions and MAPK signals pathways were upregulated in older shoots,which indicates that older shoots suffered from more pathogen-related biotic ***:The 40-year-old sprouts still had the same vitality level as the 5-year-old sprouts,although the former had some unhealthy *** conclude that during their first 40 years of growth,regenerated stump sprouts do not begin to senesce,and that physiological age of parental trees does not significantly affect the lifespan of its clonal offspring.

关键词： Sprout Regeneration Senescence White oak vegetative propagation

Effect of mini-cutting size on adventitious rooting and morphophysiological quality of Ilex paraguariensis plantlets

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Journal of Forestry Research 2021年第2期32卷 815-822页

作者： Nathalia Pimentel Denise Gazzana Janaina de Fatima Spanevello Kelen Haygert Lencina Dilson Antônio Bisognin Programa de Pós-Graduação em Engenharia Florestal Centro de Ciências Rurais da Universidade Federal de Santa MariaAvenida Roraima1000Santa MariaRS 97105-900Brazil Curso de Graduação em Agronomia Centro de Ciências Rurais da Universidade Federal de Santa MariaAvenida Roraima1000Santa MariaRS 97105-900Brazil Departamento de Fitotecnia Centro de Ciências RuraisUniversidade Federal de Santa MariaAv.Roraima1000Santa MariaRS 97105-900Brazil

As the energizing and therapeutic effects of infusions of mate(Ilex paraguariensis ***.-Hil)leaves are increasingly recognized,rising global consumption is also increasing the demand for plantlets with high genetic and morphological ***-cuttings are an alternative for clonal plantlets;however,more details are needed on factors that affect the success of this ***,toward maximizing production,we evaluated adventitious rooting competence of mini-cuttings of different sizes and the morphophysiological quality of the resultant ***(one bud;length up to 2.5 cm),medium(multiple buds;length 2.6–5.0 cm),and large(multiple buds;length 5.1–10.0 cm)mini-cuttings were planted in a mixture of commercial substrate,vermiculite,and coarse sand(1:1:1 v:v:v),grown in a humidity chamber,then evaluated after 45,60,75,and 90 days for survival,percentage of callus and shoot formation,number and length of shoots,percentage of rooted cuttings,and number and length of *** rooted mini-cuttings were then cultivated in commercial substrate and subsurface soil(2:1 v:v)and evaluated after 30,60,90 and 120 days of cultivation for morphophysiology of the plantlets,percentage of survival,number of leaves,shoot height,stem diameter,and ratio of shoot height to stem *** 120 days,the plantlets were evaluated for the ratio of shoot and root dry mass,total length,surface area,total volume,and mean diameter of roots and Dickson quality *** rooting of the mini-cuttings and the morphophysiological quality of the plantlets were not influenced by the size of the vegetative ***,single-bud mini-cuttings have similar adventitious rooting competence,and result in plantlets with adequate morphophysiological quality,based upon leaf development,shoot height,stem diameter and length,and surface area,volume and diameter of ***-bud mini-cuttings up to 2.5 cm long can thus be used to maximize production of mate plantlets by mini-c

关键词： Aquifoliaceae Clonal forestry Mini-cutting technique Plantlet production vegetative propagation

Effects of mother tree ages, different rooting mediums, light conditions and auxin treatments on rooting behaviour of Dalbergia sissoo branch cuttings

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Journal of Forestry Research 2011年第1期22卷 53-57页

作者： Bhupendra Singh Rajendra Yadav B. P. Bhatt Department of Forestry Post Box No-59 H.N.B. Garhwal University Srinagar Garhwal - 246 174 Uttarakhand India Chhattisgarh Minor Forest Produce Federation A-25 VIP Estate Khamhardih Shankar Nagar Raipur- 492 007. ICAR Research Complex for NEH Region Nagaland Centre Medziphema- 797 106 Nagaland India

Dalbergia sissoo Roxb. is one of the promising multipurpose tree species of South Asia. Most of the plantations of D. sissoo from seeds are facing severe threats due to the die-back disease, which ultimately causes death of this potential tree-species within a few months. vegetative propagation could avoid the die-back disease. Thirty mother trees of different age-groups of D. sissoo were selected for evaluating the rooting behaviour of branch cuttings from D. sissoo as influenced by auxins （IAA or IBA at 100, 200, 500 mg·L^-1）, ages of mother trees （10, 4 and 2 years old） and different environment conditions, i.e., different mediums （soil and sand） or light conditions （in shade and open condition）. The results show that application of IAA and IBA induced more numbers of cuttings （collected from 10-year-old mother trees） to root compared to control. Branch cuttings of D. sissoo collected from 10-year-old mother trees and planted in soil bed in open conditions had 100.0% of cuttings to root in IAA （100 mg·L^-1） and IBA （200 mg·L^-1） treatments. Both rooting medium （Soil and sand） influenced significantly （p〈0.05） on rooting response of branch cuttings. Soil medium was found to achieve maximum no. of branch cuttings to root, compared to sand medium.

关键词： Dalbergia sissoo hormonal treatments rooting medium shading vegetative propagation branch cutting

Study on Tissue Culture and Rapid propagation of Tilia amurensis

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Agricultural Biotechnology 2022年第3期11卷 18-21,28页

作者： Yinhua WANG Yuguang KONG Yingjun HE Liping YAN Dejun WU Shandong Academy of Forestry Sciences/Shandong Key Laboratory of Forest Tree Genetic Improvement Jinan 250014China Shandong Territorial and Spatial Planning Institute Jinan 250014China Laoshan Nature Reserve Yantai 264100China

To explore the establishment of a tissue culture and rapid propagation system of Tilia amurensis,the effects of basic medium and concentrations and ratios of plant growth regulators on tissue culture and rapid propagation of *** were *** results showed that 1/2 MS medium was the most suitable proliferation medium,and the proliferation coefficient could reach 13.5 after adding 0.05 mg/L 6-BA and 0.03 mg/L IBA;MS medium was the most suitable medium for strong plantlets and rooting,and the best medium for strong plantlets was MS+0.1 mg/L 6-BA+0.1 mg/L IBA+0.03 mg/L GA_(3),with which the average plantlet height reached 5.15 cm;and the best rooting medium was MS+1.0 mg/L6-BA+0.05 mg/L NAA,with which the rooting rate was 93.3%and the number of roots was 5.7 roots.

关键词： Tilia amurensis Tissue culture Medium for strong plantlets Medium for rooting vegetative propagation Rapid propagation