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Cancer stem-like cells directly participate in vasculogenic mimicry channels in triple-negative breast cancer

Cancer Biology & Medicine 2019年第2期16卷 299-311页

作者： Huizhi Sun Nan Yao Siqi Cheng Linqi Li Shiqi Liu Zhao Yang Guanjie Shang Danfang Zhang Zhi Yao Department of Pathology Tianjin Medical University Tianjin 300070 China Department of Pathology General Hospital of Tianjin Medical University Tianjin 300070 China Department of Immunology Tianjin Medical University Tianjin 300070 China

Objective: vasculogenic mimicry(VM) channels that are lined by tumor cells are a functional blood supply in malignant ***, the role of VM-initiating cells remains poorly understood. Cancer stem-like cells(CSCs) are positively correlated with VM. In this study, triple-negative breast cancer(TNBC) enriched with CSCs was used to investigate the relationship between VM and ***: The expression of several CSC markers was detected by immunohistochemistry in 100 human breast cancer *** clinical significance of CSC markers and the relationship between VM, CSCs, breast cancer subtypes, and VM-associated proteins were analyzed. CD133+ and ALDH+ human and mouse TNBC cells were isolated by FACS to examine the ability of VM formation and the spatial relationship between VM and ***: CSCs were associated with TNBC subtype and VM in human invasive breast cancer. CSCs in TNBC MDA-MB-231 cells formed more VM channels and expressed more molecules promoting VM than the non-TNBC MCF-7 cells in vitro. MDA-MB-231 cells that encircled VM channels on Matrigel expressed CD133. Moreover, CSCs were located near VM channels in the 3D reconstructed blood supply system in human TNBC grafts. The CD133+ and ALDH+ cells isolated from TA2 mouse breast cancer formed more VM channels in ***: CSCs line VM channels directly. Additionally, CSCs provide more VM-related molecules to synergize VM formation. The signaling pathways that control CSC differentiation may also be potential treatment targets for TNBC.

关键词： vasculogenic mimicry triple-negative breast cancer cancer stem-like cells ALDH1 CD 133

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Endovascular treatment of vasculogenic erectile dysfunction

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Asian Journal of Andrology 2015年第1期17卷 40-43,I0007页

作者： Edward D Kim Ryan C Owen Gregory S White Osama O Elkelany Cyrus D Rahnema University of Tennessee Graduate School of Medicine Knoxville TN USA.

The treatment of erectile dysfunction （ED） has been a fascination involving multiple medical specialities over the past century with urologic, cardiac and surgical experts all contributing knowledge toward this multifactorial disease. With the well-described association between ED and cardiovascular disease, angiography has been utilized to identify vasculogenic impotence. Given the success of endovascular drug-eluting stent （DES） placement for the treatment of coronary artery disease, there has been interest in using this same technology for the treatment of vasculogenic ED. For men with inflow stenosis, DES placement to bypass arterial lesions has recently been reported with a high technical success rate. Comparatively, endovascular embolization as an approach to correct veno-occlusive dysfunction has produced astonishing procedural success rates as well. However, after a thorough literature review, arterial intervention is only recommended for younger patients with isolated vascular injuries, typically from previous traumatic experiences. Short-term functional outcomes are less than optimal with long-term results yet to be determined. In conclusion, the hope for a minimally invasive approach to ED persists but additional investigation is required prior to universal endorsement.

关键词： erectile dysfunction impotence penile erection vasculogenic

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The Effects of All-Trans Retinoic Acid on vasculogenic Mimicry Formation Ability in CD133+ Glioma Stem Cells and Its Mechanisms

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Journal of Biosciences and Medicines 2017年第4期5卷 42-54页

作者： Ronghua Tang Chen Liang Jian Shangguan Shiwen Guo Department of Neurosurgery First Affiliated Hospital of Xi’an Jiaotong University Xi’an China

Objective: to investigate the effects of all-trans retinoic acid (ATRA) on vasculogenic mimicry formation in glioma stem cells. Methods: U87 stem cells were harvested through a suspension culture assay from the U87 cells, identified by CD133 and nestin, and counted by a flow cytometry. To investigate the VM formation ability of U87 stem cells with the treatment of various concentrations of ATRA, a Matrigel-based tube formation assay was used in the present study in vitro and tube-like structure (typical tube, TT;atypical tube AT) was observed and counted. Then the expressions of VEGF, VEGFR-2 and CD133 were measured throughout real time q-PCR, western blotting and immunofluorescence techniques. The data, presented as the mean ± standard deviation, were analyzed using SPSS software. One-way analysis of variance was used to compare groups and Fisher’s least significant difference tests were performed for subsequent comparisons between groups. P Results: Most of the harvested spheroid cells were positive for nestin and 88.4% were positive forCD133. The CD133+ U87 cells were cultured into tube like structure loaded on the top of Matrigel and the quantity of tubes was decreased under the treatment of ATRA. In addition, the expressions of VEGF, VEGFR-2 and CD133 were significantly reduced under the treatment of ATRA, particularly in the higher concentration groups (20 and 40 μmol, P Conclusions: ATRA may inhibit the establishment of VM differing from stem cells in glioma, and these effects may attribute to the effects of ATRA’s promotion of the differentiation of stem cells and/or down regulation of the expressions of proangiogenic factors VEGF and its receptor VEGFR-2. Thus, the results of the present study indicated a novel idea for the treatment of GBM and enriched the anti-glioma mechanisms of ARTA.

关键词： All-Trans Retinoic Acid vasculogenic Mimicry Glioma Stem Cells

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A new blood supply for human primary gallbladder carcinomas:vasculogenic mimicry,and its correlation with VEGF and significance

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外科研究与新技术 2010年第1期 49-58页

作者：范跃祖孙伟张文忠葛春艳 Department of Surgery Tongji Hospital of Tongji University

Objective To explore if vasculogenic mimicry (VM) exists in human primary gallbladder carcinomas and evaluate the correlation between the VM and expression of vascular epithelial growth factor (VEGF) in gallbladder carcinomas and its significance. MethodsSeventy-four carcinomas, 10 adenomas and 10 chronic inflammatory lesions of the gallbladder underwent operation and confirmed histopathologically were studied. Clinical-pathological data and survival of each patient with gallbladder carcinoma were recorded and followed-up. VM in human gallbladder carcinomas was observed under light microscope by HE staining, CD31 and PAS staining; the expression of VEGF proteins in each paraffin section of each patient in vivo was determined by Envision method of immunohistochemistry; the correlation among the VM, VEGF expression and their clinical significance in the patients with gallbladder carcinomas were analyzed and compared by Kaplan-Meier actuarial survival curves and Cox multiple factors. Results①13.5% (10/74) of human gallbladder carcinomas were found to contain VM, namely intratumoral, tumor cell-lined extracellular matrix (ECM)-rich, PAS-positive and vasculogenic-like network patterns. VM was associated with histological type (χ2=10.241,P=0.017), hepatic metastasis (χ2=4.238,P=0.042) and poor overall survival (χ2=5.722 1,P=0.016). ②Expression of VEGF was increased significantly in carcinomas with or without VM than adenomas and inflammatory lesions of the gallbladder (P<0.000 1) in vivo; VEGF expression in the gallbladder carcinomas without VM was increased significantly than that with VM (P=0.018 2). ③There is positive correlation between expression of VEGF and the gallbladder carcinomas without VM in the cases of Nevin stage (P=0.003 5), invasion depth (P=0.005 9), liver metastases (P=0.037 3) and lymph node metastases (P=0.000 1), the same correlation was only observed between expression of VEGF and the gallbladder carcinomas with VM in the cases of liver metastases (

关键词： gallbladder neoplasm vasculogenic mimicry blood vessels vascular epithelial growth factor prognosis

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Molecular regulation of vasculogenic mimicry in tumors and potential tumor-target therapy

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World Journal of Gastrointestinal Surgery 2010年第4期2卷 117-127页

作者： Yue-Zu Fan Wei Sun Yue-Zu Fan Wei Sun Department of Surgery Tongji Hospital Tongji University School of Medicine Shanghai 200065 China.

"vasculogenic mimicry(VM)",is a term that describes the unique ability of highly aggressive tumor cells to express a multipotent,stem cell-like phenotype,and form a pattern of vasculogenic-like networks in threedimensional *** an angiogenesis-independent pathway,VM and/or periodic acid-schiff-positive patterns are associated with poor prognosis in tumor ***,VM is resistant to angiogenesis ***,we will review the advances in research on biochemical and molecular signaling pathways of VM in tumors and on potential anti-VM therapy strategy.

关键词： Tumor-target therapy Signaling pathways High aggressive tumor Molecular regulation Prognosis vasculogenic mimicry

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Erectile dysfunction and central obesity： an Italian perspective

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Asian Journal of Andrology 2014年第4期16卷 581-591页

作者： Giovanni Corona Giulia Rastrelli Sandra Filippi Linda Vignozzi Edoardo Mannucci Mario Maggi Endocrinology Unit Maggiore-Bellaria Hospital Azienda-Usl Bologna Bologna Sexual Medicine and Andrology Unit Department of Experimental Clinical and Biomedical Sciences University of Florence Florence Diabetes Agency Careggi Hospital Florence Italy.

Erectile dysfunction （ED） is a frequent complication of obesity. The aim of this review is to critically analyze the framework of obesity and ED, dissecting the connections between the two pathological entities. Current clinical evidence shows that obesity, and in particular central obesity, is associated with both arteriogenic ED and reduced testosterone （T） levels. It is conceivable that obesity-associated hypogonadism and increased cardiovascular risk might partially justify the higher prevalence of ED in overweight and obese individuals. Conversely, the psychological disturbances related to obesity do not seem to play a major role in the pathogenesis of obesity-related ED. However, both clinical and preclinical data show that the association between ED and visceral fat accumulation is independent from known obesity-associated comorbidities. Therefore, how visceral fat could impair penile microcirculation still remains unknown. This point is particularly relevant since central obesity in ED subjects categorizes individuals at high cardiovascular risk, especially in the youngest ones. The presence of ED in obese subjects might help healthcare professionals in convincing them to initiate a virtuous cycle, where the correction of sexual dysfunction will be the reward for improved lifestyle behavior. Unsatisfying sexual activity represents a meaningful, straightforward motivation for consulting healthcare professionals, who, in turn, should take advantage of the opportunity to encourage obese patients to treat, besides ED, the underlying unfavorable conditions, thus not only restoring erectile function, but also overall health.

关键词： androgens erectile dysfunction impotence vasculogenic

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Twenty years after:the beautiful hypothesis and the ugly facts

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Chinese Journal of Cancer 2016年第2期35卷 97-99页

作者： Francesco Pezzella Kevin Gatter Chao-Nan Qian Nuffield Division of Clinical Laboratory Science Radcliffe Department of MedicineJohn Radcliffe HospitalUniversity of Oxford State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer Center

The limited clinical benefits from current antiangiogenic therapy for cancer patients have triggered some critical thoughts and insightful investigations aiming to further elucidate the relationship between vessels and *** need blood perfusion but there are mounting evidences that angiogenesis alone does not explain it in all the *** this editorial,for a special issue on tumor and vessels published in the Chinese Journal of Cancer,we briefly introduce the history of the evidences that solid tumors can sometimes obtain blood perfusion by alternative approaches other than sprouting angiogenesis,i.e.,vessel co-option and vasculogenic *** editorial provides also the links to several most recently published discoveries and hypotheses on tumor interaction with blood vessels.

关键词： Angiogenesis, vasculogenic mimicry, Vessel co-option, Treatment resistance, Targeted therapy

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