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Deproteinized bone with VEGF gene transfer to facilitate the repair of early avascular necrosis of femoral head of rabbit

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Chinese Journal of Traumatology 2009年第5期12卷 -页

作者： CAO Kai HUANG Wei AN Hong JIANG Dian-ming SHU Yong HAN Zhi-min Department of Orthopedics First Affiliated Hospital Nanchang University Nanchang 330006 China Department of Orthopedics First Affiliated Hospital Chongqing University of Medical Sciences Chonqing 400016 Chongqing China

Objective: To explore a new method for early avascular necrosis of femoral head (AVNFH) ***: Sixty-nine AVNFH New Zealand adult rabbits were randomly divided into three groups with equal number. In Group A, deproteinized bone (DPB) that absorbed with recombinant plasmid pcDNA3.1-hVEGF165 was implanted into the drilled tunnel of necrotic femoral head. In Group B, only DPB was implanted. In Group C, only tunnel was drilled without DPB or plasmid implanted. Femoral head specimens were obtained at postoperative 1, 2, 4, 8, 16 weeks. The expression of VEGF165 and collagen I was detected by immunohistochemistry. Bone formation was detected generally by X-ray. Angiogenesis and the repair of the femoral head were observed ***: The expression of VEGF 165 could be detected 2 weeks after implantation in Group A, but it was not observed in other groups. The result of collagen I expression had a significantly difference 2, 4 and 8 weeks after operation in Group A from those in other groups (P＜0.01).X-ray results indicated that there was more bone formation in Group A than in other groups. The regenerated capillary vessels staining result of necrotic femoral head in Group A was significantly different from those in other groups at postoperative 2 and 4 weeks (P＜0.01).Conclusions: Transfection ofhVEGF165 gene enhances local angiogenesis and DPB-VEGF compound improves the repair of necrotic femoral head. Deproteinized bone grafting with VEGF gene transfer provides a potential method for the treatment of osteonecrosis.

关键词： vascularendothelialgrowthfactor Gene therapy Femoral head necrosis

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Cleavage of hairpin ribozyme to target a transcribed VEGF RNA in vitro

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Journal of Medical Colleges of PLA(China) 2001年第2期16卷 102-106页

作者：严瑞兰金明钱新华惠宏襄辛晓燕王健王检波 Department of Gynecology and Obstetrics Xijing Hospital Fourth Military Medical University Xi'an 710032 China Department of Biochemistry and Molecular Biology Fourth Military Medical University Xi'an 710032 China Department of Pediatrics First Military Medical Mniversty Guangzhou 510060 China Department of Pathology Fourth Military Medical University Xi'an 710032 China

Objective:To constructan expressionvectorbearinghairpinribozyme(Rz)geneagainstvascularendo-thelialgrowthfactor(VEGF)andto assaythecleavageactivityof theribozymein *** :Anti-VEGFhair-pinRz genewassynthesizedwhileVEGFgenewasclonedfromhumanplacenta,andtheywerebothinsertedinto ***3-RzandthepcDNA-VEGFweretranscriptedin vitro and cleavageactivityof theRz ***:Thefragmentof theRz genewasconfirmedby thedigestionof pcDNA3-RzwithEcoR I andBam *** ***:WehavesuccessfullyconstructedpcDNA3-Rzexpressionvectorandlaida basisforfurtherstudyof gene therapy.

关键词： vascularendothelialgrowthfactor hairpinribozyme genetherapy

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