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Knockdown of decoy Receptor 3 Impairs growth and Invasiveness of Hepatocellular Carcinoma Cell Line of HepG2

Chinese Medical Journal 2016年第21期129卷 2623-2629页

作者： Xiao-Na Zhou Guang-Ming Li Ying-Chen Xu Tuan-Jie Zhao Ji-Xiang Wu department of General Surgery Beijing Tongren Hospital Capital Medical University Beijing 100730 China department of General Surgery Beijing Er Long Lu Hospital Beijing 100032 China

Background：decoy receptor 3 （dcR3） binds to Fas ligand （FasL） and inhibits FasL-induced *** receptor is overexpressed in hepatocellular carcinoma （HCC）,and it is associated with the growth and metastatic spread of ***3 holds promises as a new target for the treatment of HCC,but little is known regarding the molecular mechanisms underlying the oncogenic properties of *** present work,therefore,examined the role of dcR3 in regulating the growth and invasive property of liver cancer cell ***：HepG2 cells were stably transfected with lentivirus-based short hairpin RNA vector targeting *** the knockdown of dcR3 was confirmed,cell proliferation,clone formation,ability of migrating across transwell membrane,and wound healing were assessed in *** metalloproteinase-9 （MMP 9） and vascular epithelial growth factor （VEGF）-C and d expressions of the dcR3 knockdown were also *** between multiple groups were done using one-way analysis of variance （ANOVA）,while pairwise comparisons were performed using Student＇s t test.P 〈 0.05 was regarded statistically ***：dcR3 was overexpressed in HepG2 compared to other HCC cell lines and normal hepatocyte *** knockdown of dcR3 slowed down the growth of HepG2 （P 〈 0.05） and reduced the number of clones formed by 50% compared to those without dcR3 knockdown （P 〈 0.05）.The knockdown also reduced the migration of HepG2 across transwell matrix membrane by five folds compared to the control （P 〈 0.05） and suppressed the closure of scratch wound （P 〈 0.05）.In addition,the messenger RNA levels of MMP 9,VEGF-C,and VEGF-d were significantly suppressed by dcR3 knockdown by 90% when compared with the mock control （P 〈 0.05）.Conclusions：Loss of dcR3 impaired the growth and invasive property of HCC cell line of *** dcR3 may be a potential therapeutic approach for the treatment of HCC.

关键词： decoy Receptor 3 Hepatocellular Carcinonia Matrix Metalloproteinase 9 Neoplasm Metastasis vascular endothelial growth factor C vascular endothelial growth factor d

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