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Pan-Specific Antibodies as Novel Tools to Detect valyllysine

Reproductive and Developmental Medicine 2020年第2期4卷 78-83页

作者： Ya-Ling Wang Min-Yan Liu Yu-Hua Li Yi-TingYang Wei-Wei Wang He-GuoYu Zhi-Yu Shao Hua Diao Department of Reproductive Biology NHC Key Laboratory of Reproduction Regulation(Shanghai Institute of Planned Parenthood Research)School of Basic Medical SciencesFudan UniversityShanghai 200032China College of Chemistry Chemical Engineering and BiotechnologyDonghua UniversityShanghai 201620China

Objective:Amino acyl modification of lysine residues is an essential mechanism of nutrient sensing that regulates various biological functions including *** present,the lack of pan-specific antibodies for a recently identified lysine valylation hinders the characterization and detection of this *** objective of this study is to raise pan-specific antibodies that may facilitate the identification of novel expression patterns of lysine ***:Chicken ovalbumin was valylated as an immunogen to raise polyclonal antibodies(PcAbs)in *** population of the pan-specific antibodies recognizing valylated lysine was purified using the chemically synthesized valylated peptides consisting of random amino *** specificity of the antibodies was evaluated using ELISA,dot blots,Western blots,and immunohistochemistry(IHC)staining in human epididymis as ***:A preliminary and simple strategy to make an anti-valylated lysine PcAb was *** recognition of the antibodies to valyllysine was evaluated as pan *** was useful for the detection of the newly identified valyl modification using ELISA,dot blots,and Western *** antibodies were also successfully utilized in IHC assays,which revealed novel valyllysine modification patterns in epididymis tissues of ***:A new antibody tool was provided for the study of lysine *** novel expression patterns of valyllysine in the epididymis suggest that this modification may be involved in sperm maturation.

关键词： Anti-valyllysine Antibody Epididymis Posttranslational Modifications valyllysine

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