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Donkey pericardium as a select sourcing to manufacture percutaneous heart valves:Decellularization has not yet demonstrated any clear cut advantage to glutaraldehyde treatment

引用

Medicine in Novel Technology and Devices 2019年第4期4卷 38-46页

作者： Aisa Rassoli Yiming Li Xianhao Bao Fabien Kawecki Xinzhe Zhao Daniel Chappard Gaetan Le-Bel Jiaxuan Feng Benedikt Weber Nasser Fatouraee Ze Zhang Zaiping Jing Lucie Germain Lu Wang Robert Guidoin Department of Surgery Faculty of MedicineUniversite Laval and Regenerative Medicine AxisCHU de Quebec Research CenterQuebecCanada Department of Biomedical Engineering Amirkabir University of TechnologyTehranIran Departnent of Vascular Surgery Changhai Hospital of Naval Military Medical UniversityShanghaiChina Key Laboratory of Textile Sciences and Technology Ministry of EducationColle of TextilesDonghua UniversityShanghaiChina GEROM Institut de Biologie en SanteCHU D'AngersAngersFrance Department of Dermatology Medical University of ViennaViennaAustria

Background:The donkey pericardium is considered a good candidate to manufacture percutaneous heart valves based upon its thinness,low cellularity and undulating collagen bundles and *** represents an avenue worth exploring,should its superiority to glutaraldehyde-treated pericardium be *** and methods:Donkey pericardium was divided into two groups:regular glutaraldehyde fixation and mild *** treated pericardia were observed using scanning electron microscopy,histology and transmission electron *** tests were performed along the axial and perpendicular directions,with the data fitted into both the Gasser–Ogden–Holzapfel(GOH)material model and the Fung’s anisotropic ***:The microstructures of the pericardia processed by the two protocols were similar,showing collagen bundles and laminates free of *** decellularization eliminated most of the cells,however leaving the structure somehow *** collagen filaments in bundles were slightly *** anisotropy rates of the non-decellularized specimens were almost identical to the decellularized *** decellularized pericardium appeared ***:The decellularization proved to be ***,it makes the tissue stiffer,which may lead to higher shear concentration during cardiac cycles and reduce its wavy ***,it appears premature to select decellularized donkey pericardium to manufacture heart valves.

关键词： Donkey pericardium Decellularization valve leaflet Transcatheter aortic valve implantation Wavy microstructure

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