检索结果-南通市图书馆

Hyperpolarization-activated cyclic nucleotide-gated cation channel subtypes differentially modulate the excitability of murine small intestinal afferents

引用

World Journal of Gastroenterology 2012年第6期18卷 522-531页

作者： Ying-Ping Wang Bi-Ying Sun Qian Li Li Dong Guo-Hua Zhang David Grundy Wei-Fang Rong Department of Physiology Shanghai Jiaotong University School of MedicineShanghai 200025China Department of Biomedical Science University of SheffieldSheffield S10 2TNUnited Kingdom

AIM： To assess the role of hyperpolarization-activated cyclic nucleotide-gated cation （HCN） channels in regu- lating the excitability of vagal and spinal gut afferents. METHODS： The mechanosensory response of mesen- teric afferent activity was measured in an ex vivo murine jejunum preparation. HCN channel activity was recorded through voltage and current clamp in acutely dissoci- ated dorsal root ganglia （DRG） and nodose ganglia （NG） neurons retrogradely labeled from the small intestine through injection of a fluorescent marker （DiI）. The isoforms of HCN channels expressed in DRG and NG neurons were examined by immunohistochemistry. RESULTS： Ramp distension of the small intestine evok- ed biphasic increases in the afferent nerve activity, re- flecting the activation of low- and high-threshold *** blocker CsCl （5 mmol/L） preferentially inhibited the responses of low-threshold fibers to distension and showed no significant effects on the high-threshold re- sponses. The effect of CsCI was mimicked by the more selective HCN blocker ZD7288 （10 ~mol/L）. In 71.4% of DiI labeled DRG neurons （/7 = 20） and 90.9% of DiI labeled NG neurons （n = 10）, an inward current （Ih current） was evoked by hyperpolarization pulses which was fully eliminated by extracellular CsCI. In neurons expressing Ih current, a typical ＂sag＂ was observed upon injection of hyperpolarizing current pulses in cur- rent-clamp recordings. CsCI abolished the sag entirely. In some DiI labeled DRG neurons, the Ih current was potentiated by 8-Br-cAMP, which had no effect on the Ih current of DiI labeled NG neurons. Immunohistochem- istry revealed differential expression of HCN isoforms in vagal and spinal afferents, and HCN2 and HCN3 seemed to be the dominant isoform in DRG and NG, respec- ***： HCNs differentially regulate the excit- ability of vagal and spinal afferent of murine small in- testine.

关键词： Hyperpolarization-activated cyclic nucleo-tide-gated cation vagal afferent Spinal afferent Gas-trointestinal tract CsCI

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Upregulated HCN2-mediated signaling contributes to TNBS-induced post-inflammatory hypersensitivity of the murine small intestine

Upregulated HCN2-mediated signaling contributes to TNBS-indu...

引用

2012国际生理学学术大会

作者： Yingping Wang Biying Sun Qian Li Li Dong Guohua Zhang Weifang Rong Department of Physiology Shanghai Jiaotong University School of Medicine

We previously demonstrated a differential expression of hyperpolarization-activated cyclic nucleotidegated cation channels,HCN2 and HCN3,in spinal and vagal afferents of the murine *** we explore the role of HCNs in TNBS-induced visceral *** distension(to 60 mmHg) of ex vivo jejunum segments from naive or TNBS-treated(50 mg/kg,1 to 8 weeks prior to experiment) mice both evoked biphasic increases in mesenteric afferent nerve activity,reflecting the activation of low and high threshold *** threshold mechanosensory responses(HTRs) were greater in TNBS-treated than in native *** blocker ZD7288(10μM) inhibited the low threshold mechanosensory responses(LTRs) in naive preparations but in contrast attenuated HTRs in TNBS-treated *** modulator cAMP(1 mM) augmented HTRs in TNBS-treated preparations and was without effect on the mechanosensory activity of naive *** activator Sp-cAMPS(30μM) didn’t alter the mechanosensory activity of naive or TNBS-treated *** the presence of PKA inhibitor,Rp-cAMPS(12μM),cAMP still potentiated HTRs of TNBS-treated preparations. These results together with histological data suggest that upregulation of HCN-mediated signaling might contribute to TNBS-induced post-inflammatory small intestinal hypersensitivity.

关键词： HCN spinal afferent vagal afferent mechanosensitivity

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：