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Trabecular-like Ti-6Al-4V scaffolds for orthopedic: fabrication by selective laser melting and in vitro biocompatibility

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Journal of Materials Science & Technology 2019年第7期35卷 1284-1297页

作者： Huixin Liang Youwen Yang Deqiao Xie Lan Li Ning Mao Changjiang Wang Zongjun Tian Qing jiang Lida Shen College of Mechanical and Electrical Engineering Nanjing University of Aeronautics and AstronauticsNanjing 210016China State Key Laboratory of High Performance Complex Manufacturing Central South UniversityChangsha 410083China Jiangxi University of Science and Technology Ganzhou 341000China Department of Sports Medicine and Adult Reconstructive Surgery Nanjing University Medical School Affiliated Drum Tower HospitalNanjing 210008China Department of Engineering and Design University of SussexSussex HouseBrighton BN19RHUnited Kingdom Suzhou fCangli Orthopedics Instrument Co. Ltd. Suzhou 215624China

Porous metal scaffolds play an important role in the orthopedic field, due to their wide applications in prostheses implantation. Some previous studies showed that the scaffolds with trabecular bone structure reconstructed via computed tomography had satisfactory biocompatibility. However, the reverse modeling scaffolds were inflexible for customized design. Therefore, a top-down designing biomimetic bone scaffold with favorable mechanical performances and cytocompatibility is urgently demanded for orthopedic implants. An emerging additive manufacturing technique, selective laser melting, was employed to fabricate the trabecular-like porous Ti-6Al-4 V scaffolds with varying irregularities(0.05-0.5) and porosities(48.83%–74.28%) designed through a novel Voronoi-Tessellation based method. Micro-computed tomography and scanning electron microscopy were used to characterize the scaffolds’ ***-static compression tests were performed to evaluate the scaffolds’ mechanical properties. The MG63 cells culture in vitro experiments, including adhesion, proliferation, and differentiation, were conducted to study the cytocompatibility of scaffolds. Compressive tests of scaffolds revealed an apparent elastic modulus range of 1.93–5.24 GPa and an ultimate strength ranging within 44.9–237.5 MPa, which were influenced by irregularity and porosity, and improved by heat treatment. Furthermore, the in vitro assay suggested that the original surface of the SLM-fabricated scaffolds was favorable for osteoblasts adhesion and migration because of micro scale pores and ravines. The trabecular-like porous scaffolds with full irregularity and higher porosity exhibited enhanced cells proliferation and osteoblast differentiation at earlier time, due to their preferable combination of small and large pores with various shapes. This study suggested that selective laser melting-derived Ti-6Al-4 V scaffold with the trabecular-like porous structure designed through Voronoi-Tessellation m

关键词： Irregular porous structure Selective laser melting Voronoi-Tessellation Mechanical performance In vitro study

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Infectious in vitro transcripts from cloned cDNA of beet necrotic yellow vein virus RNA3 and RNA4and their functional study

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Science China Chemistry 1995年第4期38卷 438-447页

作者：李毅魏春红田波潘乃穟陈章良 State Key Laboratory for Protein Engineering and Plant Genetic Engineering Department of Biology Peking University Beijing 100871 China Institute of Microbiology Chinese Acaderny of SciencesBeijing 100080China

The full-length double-stranded cDNAs of beet necrotie yellow vein virus RNA3 and RNA 4were synthesized by using oligo(dT) 15 as well as RNA3 and RNA4 specific primers, and cloned downstream of the bacteriophage Sp6 RNA polymerase promoter of the transcription vector pGEM3Zf(+). The in vitro "run-off" transcription products obtained in the presence of Sp6 RNA polymerase and template DNA have high biological activities. In the 2 transcription systems, the transcription and capping in 2 separate reactions are more efficient. The simultaneous transcription and capping are not so efficient, but the transcripts are more infectious. Although there are a number of nonviral nucleotide sequences at the 5- and 3’-ends of RNA3 and RNA4 transcripts, the biological activities of both transcripts were not affected. The mechanical coinoculation of sugarbeet roots with the infectious transcripts of the prepared RNA3 and RNA4 and BNYW Rgl isolate has confirmed that RNA3 is the main cause of sugarbeet rhizomania.

关键词： beet necrotic yellow vein virus in vitro transcription function analysis.

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Preservation of Juniperus thurifera L.:a rare endangered species in Algeria through in vitro regeneration

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Journal of Forestry Research 2019年第1期30卷 77-86页

作者： Nadia Khater Halima Benbouza Faculté SNV Université Batna 2 Institut des Sciences Vétérinaires et Sciences Agronomiques Université Batna 1 Centre de Recherche en Biotechnologies

Juniperus thurifera *** an endemic Cupres saceae from the Aure`s Mountains of north eastern Algeria and endangered,in part,due to the scarcity of viable seeds It is threatened by other abiotic factors and the lack of an effective management strategy will increase its risk o *** dearth of information on its in vitro regeneration impedes its application in forest managemen *** therefore developed a micropropagation protocol using microcuttings with auxiliary *** were grown on different combinations of media supplemented with plant growth regulators at different *** highest number of shoots and branches regenerated from original shoots was obtained on Woody Plant Medium(WPM)supplemented with 6-benzylaminopurine(BAP)(0.5 mg L^(-1))and 2,4-dichlorophe noxyacetic acid(2,4-D)(0.25 mg L^(-1)).The best elongation of shoots was achieved with WPM supplemented with0.5 mg L^(-1)of BAP and 0.25 or 1 mg L^(-1) of 2,*** the second subculture,shoots had a higher number of branches than those of the *** highest rooting rate,38.8%,was obtained with shoots cultured in 1/2 Murashige and Skoog(MS)medium supplemented with 5.0 mg L^(-1)each of indol-3-butyric(IBA)and naphthalene acetic acid(NAA).Similarly,the highest root numbers and lengths were produced on 1/2 MS medium supplemented with IBA and NAA(5.0 mg L^(-1)each).During transfer to acclimatization,rates of plant losses of 50% *** second part of the experiment showed that the best shoot callusing was on WPM supplemented with BAP and 2,4-D,with either the combination 0.5+0.25 or 0.25+0.25 mg L^(-1).The results of this research provide a starting point for further studies on in vitro regeneration of *** for the sustainable management of its unique ecosystem in the Mediterranean basin.

关键词： Juniperus thurifera Callus Conservation Regeneration In vitro culture

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Hyodeoxycholic acid protects the neurovascular unit against oxygen-glucose deprivation and reoxygenation-induced injury in vitro

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Neural Regeneration Research 2019年第11期14卷 1941-1949页

作者： Chang-Xiang Li Xue-Qian Wang Fa-Feng Cheng Xin Yan Juan Luo Qing-Guo Wang School of Traditional Chinese Medicine Beijing University of Chinese Medicine

Calculus bovis is commonly used for the treatment of stroke in traditional Chinese medicine. Hyodeoxycholic acid(HDCA) is a bioactive compound extracted from calculus bovis. When combined with cholic acid, baicalin and jas-minoidin, HDCA prevents hypoxia-reoxygenation-induced brain injury by suppressing endoplasmic reticulum stress-mediated apoptotic signaling. However, the effects of HDCA in ischemic stroke injury have not yet been studied. Neurovascular unit(NVU) dysfunction occurs in ischemic stroke. Therefore, in this study, we investigated the effects of HDCA on the NVU under ischemic conditions in vitro. We co-cultured primary brain microvascular endothelial cells, neurons and astrocytes using a transwell chamber co-culture system. The NVU was pre-treated with 10.16 or 2.54 μg/mL HDCA for 24 hours before exposure to oxygen-glucose deprivation for 1 hour. The cell counting kit-8 assay was used to detect cell activity. Flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling were used to assess apoptosis. Enzyme-linked immunosorbent assay was used to measure the expression levels of inflammatory cytokines, including interleukin-1β, interleukin-6 and tumor necrosis factor-α, and neurotrophic factors, including brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor. Oxidative stress-related factors, such as superoxide dismutase, nitric oxide, malondialdehyde and γ-glutamyltransferase, were measured using kits. Pretreatment with HDCA significantly decreased blood-brain barrier permeability and neuronal apoptosis, significantly increased transendothelial electrical resistance and γ-glutamyltransferase activity, attenuated oxidative stress damage and the release of inflammatory cytokines, and increased brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor expression. Our findings suggest that HDCA maintains NVU morphological integrity and function by modulating inflammation, oxidat

关键词： hyodeoxycholic acid oxygen glucose deprivation and reoxygenation blood-brain barrier permeability anti-oxidative anti-inflammatory anti-apoptotic brain-derived neurotrophic factor glial cell line-derived neurotrophic factor ischemic stroke in vitro neurovascular unit

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Growth-inhibiting effects of taxol on human liver cancer in vitro and in nude mice

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World Journal of Gastroenterology 2000年第2期6卷 210-215页

作者： Jin Hui Yuan Ru Ping Zhang Ru Gang Zhang Li Xia Guo Xing Wang Wang Dan Luo Yong Xie Hong Xie Shanghai Institute of Cell Biology Chinese Academy of SciencesShanghai 200031China Hong Kong University of Science and Technology Hong KongChina

AIM To investigate the effects of taxol onSMMC-7721 human hepatoma and *** In vitro cell growth was assessedby trypan blue exclusion *** model was established by seedingSMMC-7721 cells subcutaneously into Balb/c(nu/nu)nude *** vivo tumor growth wasdetermined by measurement of tumor diameterwith Vernier *** syntheses of DNA,RNA and protein were analyzed by incorporationof ~3H-thymidine,~3H-uridine and ~*** light and electronmicroscopes to observe the morphologicalchanges of cells including mitosis *** Taxol was effective against SMMC-7721 human hepatoma cell growth in the rangesof 2.5 nmol/L-10 nmol/L with mitotic arrestand apoptosis in ***,RNA and proteinsyntheses in cells were also obviouslysuppressed by in vitro treatment of taxol for72 *** at 2.5 nmol/L reduced ~3H-thymidineuptake to about 34% of the control value(Pvitro and in nude *** mechanisms might beassociated with mitotic arrest,subsequently,apoptosis of the hepatoma *** obvioustoxicity was observed with in vivoadministration of taxoi.

关键词： paclitaxel liver neoplasms apoptosis mitoics in vitro DNA RNA microscopy wection mice nude

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High in vitro survival rate of sheep in vitro produced blastocysts vitrified with a new method and device

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Journal of Animal Science and Biotechnology 2020年第1期11卷 71-80页

作者： Sergio Ledda Jen M.Kelly Stefano Nieddu Daniela Bebbere Federica Ariu Luisa Bogliolo Dity Natan Amir Arav Department of Veterinary Medicine University of SassariSassariItaly South Australian Research and Development Institute Turretfield Research Centre129 Holland RoadRosedaleSA 5350Australia FertilSafe Ltd 11 Haharash st7403118 Ness ZionaIsrael.

Background:To advance the use of embryo vitrification in veterinary practice,we developed a system in which embryo vitrification,warming and dilution can be performed within a *** in vitro produced embryos(IVEP)were vitrified at either early(EBs:n=74)or fully expanded blastocyst stage(FEBs:n=195),using a new device named"***",composed by a 0.25-m L straw with a 50-μm pore polycarbonate grid at one *** at each stage(EBs and FEBs)were vitrified by either Two-step(TS)or Multi-step(MS;6 different concentrations of vitrification solutions)***-vitrified embryos(n=102)were maintained in in vitro culture as a *** consisted of placing the straws directly into 1.5 m L tubes containing a TCM-199 solution with three decreasing concentrations of *** re-expansion,embryo survival and hatching rate were evaluated at2,24 and 48 h post *** number of apoptotic cells was determined by TUNEL ***:Blastocyst re-expansion(2 h)after warming was higher(Pvitro comparable with control fresh *** method has the potential for use in direct embryo transfer in field conditions.

关键词： Blastocysts High survival In-straw In vitro embryo produced Ovine

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Transcription reactions of yeast RNA polymerase II in vitro

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Science China Chemistry 1995年第6期38卷 697-704页

作者：赵宇敖世洲 State Key Laboratory of Molecular Biology Shanghai Institute of Biochemistry Chinese Academy of Sciences Shanghai 200031 China

The transcription reactions in vitro of yeast ADHl and PHO5 gene promoters are investigated by means of a yeast crude nuclear extract. Using specific RNA probes, the transcription products of these 2 promoters have been first obtained. A low concentration of α-amanitin is highly inhibitory. The transcription of the PHO5 gene was initiated in vitro at or near the sites used in vim. The transcription products increase with the amount of the template and reach the maximum at certain concentrations of the template. The deletion of the yeast promoter sequences abolishes the reaction.

关键词： transcription in vitro ADHl and PHO5 promoters αα-amanitin RNA probes.

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Application of custom anatomy-based nerve conduits on rabbit sciatic nerve defects: in vitro and in vivo evaluations

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Neural Regeneration Research 2019年第12期14卷 2173-2182页

作者： Yamuhanmode·Alike Maimaiaili·Yushan Ajimu·Keremu Alimujiang·Abulaiti Zhen-Hui Liu Wei Fu Li-Wei Yan Aihemaitijiang·Yusufu Qing-Tang Zhu Department of Microrepair and Reconstruction the First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang Uygur Autonomous Region China Orthopedic Center the First People’s Hospital of Kashgar Kashi Xinjiang Uygur Autonomous Region China Department of Microsurgery and Orthopedic Trauma the First Affiliated Hospital of Sun Yat-sen University Guangzhou Guangdong Province China Center for Peripheral Nerve Tissue Engineering and Technology Research Guangzhou Guangdong Province China Guangdong Province Engineering Laboratory for Soft Tissue Biofabrication Guangzhou Guangdong Province China

The intermingling of regenerated nerve fibers inside nerve grafts is the main reason for mismatched nerve fibers. This is one of the key factors affecting limb function recovery after nerve injury. Previous research has shown that the accuracy of axon regeneration can be improved by a bionic structural implant. To this aim, iodine and freeze-drying high-resolution micro-computed tomography was performed to visualize the 3D topography of the New Zealand rabbit sciatic nerve (25 mm). A series of 1-, 2-, 3-, and 4-custom anatomy-based nerve conduits (CANCs) were fabricated based on the anatomical structure of the nerve fascicle. The match index, luminal surface, and mechanical properties of CANCs were evaluated before implanting in a 10-mm gap of the sciatic nerve. Recovery was evaluated by histomorphometric analyses, electrophysiological study, gastrocnemius muscle weight recovery ratio, and behavioral assessments at 12 and 24 weeks postoperatively. The accuracy of nerve regeneration was determined by changes in fluorescence-labeled profile number during simultaneous retrograde tracing. Our results showed that the optimal preprocessing condition for high-resolution micro-computed tomography visualization was treatment of the sciatic nerve with 40% Lugol’s solution for 3 days followed by lyophilization for 2 days. In vitro experiments demonstrated that the match index was highest in the 3-CANC group, followed by the 2-, 1-, and 4-CANC groups. The luminal surface was lowest in the 1-CANC group. Mechanical properties (transverse compressive and bending properties) were higher in the 3- and 4-CANC groups than in the 1-CANC group. In vivo experiments demonstrated that the recovery (morphology of regenerated fibers, compound muscle action potential, gastrocnemius muscle weight recovery ratio, pain-related autotomy behaviors, and range of motion) in the 3-CANC group was superior to the other CANC groups, and achieved the same therapeutic effect as the autograft. The simultan

关键词： nerve regeneration nerve conduits mismatch iodine and freeze-drying high-resolution micro-computed tomography bio-mimic custom rabbit sciatic nerve in vitro in vivo neural regeneration

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Comparative Toxicity of Nanomaterials to Air-blood Barrier Permeability Using an In vitro Model

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Biomedical and Environmental Sciences 2019年第8期32卷 602-613页

作者： ZHAO Kang Feng SONG Yu Qing ZHANG Rui Hua YANG Xiao Yan SUN Bo HOU Zhi Quan PU Xiao Ping DAI Hong Xing BAI Xue Tao Department of Environmental Toxicology National Institute of Environmental HealthChinese Center for Disease Control and PreventionBeijing 100021China Peking Union Medical College Beijing 100730China Research Institute of Chemical Defence Beijing 100083China Department of Chemical Engineering School of Environment and Energy EngineeringBeijing University of Technology Beijing 100124China Department of Molecular and Cellular Pharmacology School of Pharmaceutical SciencesPeking UniversityBeijing 100083China

Objective To comparatively study the toxicity of four metal-containing nanoparticles(MNPs) and their chemical counterparts to the air-blood barrier(ABB) permeability using an in vitro *** ABB model, which was developed via the co-culturing of A549 and pulmonary capillary endothelium, was exposed to spherical CuO-NPs(divided into CuO-40, CuO-80, and CuO-100 based on particle size), nano-Al2O3(sheet and short-rod-shaped), nano-ZnO, nano-Pb S, CuSO4, Al2(SO4)3, Zn(CH3COO)2, and Pb(NO3)2 for 60 *** 10 min following exposure, the cumulative cleared volume(ΔTCL) of Lucifer yellow by the model was calculated.A clearance curve was established using linear regression analysis of ΔTCL versus *** coefficient(P) was calculated based on the slope of the curve to represent the degree of change in the ABB *** The results found the increased P values of CuO-40, CuO-80, sheet, and short-rod-shaped nano-Al2O3, Al2(SO4)3, and Pb(NO3)*** them, small CuO-40 and CuO-80 were stronger than CuO-100 and CuSO4;no difference was observed between Al2(SO4)3 and sheet and short-rod-shaped nano-Al2O3;and nano-Pb S was slightly weaker than Pb(NO3)*** clearly the MNPs possess diverse *** ABB permeability abnormality means pulmonary toxicity *** studies are warranted to understand MNPs toxicity and ultimately control the health hazards.

关键词： Air-blood barrier In vitro model Permeability Metal-containing nanoparticles (MNPs) Toxicity screening

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Synthesis and in vitro evaluation of new diphenyl ether derivatives as serotonin transporter ligands

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Science China Chemistry 2008年第5期51卷 457-463页

作者： DEUTHER-CONRAD Winnie BRUST Peter STEINBACH Jrg VERCOUILLIE Johnny Institute of Interdisciplinary Isotope Research

For the development of new ligands as potential imaging agents for the serotonin transporter (SERT),a series of diphenyl ether derivatives have been synthesized,characterized,and evaluated for their in vitro binding affinities to the SERT. Among the above compounds,2-(2-((dimethylamino)methyl)-4-fluoro-phenoxy)-5-bromobenzenamine (15) and 2-(2-((dimethylamino)methyl)-4-fluorophenoxy)-5-iodobenzene amine (16) show high binding affinities for the SERT with Ki values of 0.28 and 0.20 nmol·L-1,respectively. They can be further labeled with carbon-11,fluorine-18,iodine-123 or bromine-76,and evaluated as useful imaging agents for the SERT. Moreover,the study of the structure-activity relationship (SAR) provides some useful information for the future design of new ligands.

关键词： serotonin transporter diphenyl ether imaging agents in vitro evaluation

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