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Roles of Salicylic Acid-responsive Cis-acting Elements and W-boxes in Salicylic Acid Induction of vch3 promoter in Transgenic Tobaccos

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Acta Biochimica et Biophysica Sinica 2006年第1期38卷 46-52页

作者： Hai-Yan LI~(1,2),Wei WEI~(2&),and Yu LI~(1*) ~1 Institute of Mycology,Jilin Agricultural University,Changchun 130118,China ~2 College of Biotechnology,Jilin Agricultural University,Changchun 130118,China Institute of Mycology Jilin Agricultural University Changchun 130118 China College of Biotechnology Jilin Agricultural University Changchun 130118 China

A salicylic acid (SA)-inducible vch3 promoter was recently identified from grapevine (Vitisarnurensis) that contains two inverse SA-responsive cis-acting elements and four *** furtherdemonstrate the roles of these elements,four fragments with lengths from-1187,-892,-589,-276 to+7 bp were fused with the β-glucuronidase (GUS) reporter géne and transferred to Nicotiana tobacum,together with another four vch3 promoter fragments with mutation in the two inverse *** functions,of each promoter fragment were-examined by analysis of GUS activity in thetransgenic tobacco root treated with *** GUS activity was shown in the roots of transgenictobaccos with the vch3 (-1187)-GUS construct containing two SA-responsive cis-acting elements andfour ***,GUS activity directed by the vch3 (-892)-GUS construct,containing one SA cis-acting element and four W-boxes,was reduced by up to 35% compared with that in tobaccos transformedwith the vch3 (-1187)-GUS construct,indicating that the SA cis-acting element plays an important role inSA induction of the vch3 *** the m2vch3 (-1187)-GUS nor the mvch3 (-892)-GUSconstruct,with mutation on the SA-responsive elements,abolished the expression of GUS activity,demon-strating that the W-boxes in the vch3 promoter are also involved in SA *** arialysis ofGUS activity directed by each of the eight vch3 promoter fragments showed that GUS was expressedspecifically in vascular *** was concluded that both the SA-responsive cis-acting elements and the W-boxes are important for the SA induction of the vch3 *** promoter might have a potential usein plant genetic engineering.

关键词： vch3 promoter salicylic acid(SA)-responsive cis-acting element W-box site-directed mutagenesis SA induction transgenic tobacco

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